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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

Gmps

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (Gmps)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104For GATase activityPROSITE-ProRule annotation1
Active sitei190For GATase activityPROSITE-ProRule annotation1
Active sitei192For GATase activityPROSITE-ProRule annotation1
Binding sitei337SubstrateBy similarity1
Binding sitei522SubstrateBy similarity1
Binding sitei610SubstrateBy similarity1
Binding sitei685SubstrateBy similarity1
Binding sitei691SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi244 – 250ATPPROSITE-ProRule annotation7

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • enzyme binding Source: RGD
  • GMP synthase (glutamine-hydrolyzing) activity Source: RGD
  • GMP synthase activity Source: RGD
  • pyrophosphatase activity Source: InterPro

GO - Biological processi

  • glutamine metabolic process Source: UniProtKB-KW
  • GMP biosynthetic process Source: RGD
  • response to drug Source: RGD
  • response to L-cysteine Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ4V7C6.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.950.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:Gmps
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1310063. Gmps.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002843672 – 693GMP synthase [glutamine-hydrolyzing]Add BLAST692

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei8PhosphoserineBy similarity1
Modified residuei9N6-acetyllysineBy similarity1
Modified residuei318PhosphothreonineBy similarity1
Modified residuei332PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ4V7C6.

PTM databases

iPTMnetiQ4V7C6.
PhosphoSitePlusiQ4V7C6.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • enzyme binding Source: RGD

Structurei

3D structure databases

ProteinModelPortaliQ4V7C6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 216Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd BLAST190
Domaini217 – 435GMPS ATP-PPasePROSITE-ProRule annotationAdd BLAST219

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation
Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000223965.
HOVERGENiHBG005929.
InParanoidiQ4V7C6.
KOiK01951.
PhylomeDBiQ4V7C6.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
cd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_GATase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4V7C6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALCNGDSKP ENAGGDLKDG CHHYEGAVVI LDAGAQYGKV IDRRVRELFV
60 70 80 90 100
QSEIFPLETP AFAIKEQGFR AIIISGGPNS VYAEDAPWFD PAIFTIGKPV
110 120 130 140 150
LGICYGMQMM NKVFGGTVHK KSVREDGVFN ISMDNTCSLF RGLQKEEIVL
160 170 180 190 200
LTHGDSVDKV ADGFKVVARS GNIVAGIANE SKKLYGVQFH PEVGLTENGK
210 220 230 240 250
VILKNFLYDI AGCSGNFTVQ NRELECIREI KEKVGTSKVL VLLSGGVDST
260 270 280 290 300
VCTALLNRAL NQDQVIAVHI DNGFMRKRES QSVEEALKKL GIQVKVINAA
310 320 330 340 350
HSFYNGTTTL PISDEDRTPR KRISKTLNMT TSPEEKRKII GDTFVKIANE
360 370 380 390 400
VIGEMSLKPE EVFLAQGTLR PDLIESASLV ASGKAELIKT HHNDTELIRK
410 420 430 440 450
LREEGKVIEP LKDFHKDEVR ILGRELDLPE ELVSRHPFPG PGLAIRVICA
460 470 480 490 500
EEPYICKDFP ETNNILKIVA DFSASVKKPH TLLQRVKACT TEEDQEKLME
510 520 530 540 550
ITSQHSLNAF LLPIKTVGVQ GDCRSYSYVC GISSKDEPDW ESLIFLARLI
560 570 580 590 600
PRMCHNINRV VYIFGPPVKE PPTDVTPTFL TTGVLSTLRQ ADFEAHNILR
610 620 630 640 650
ESGYAGKISQ MPVILTPLHF DRDPLQKQPS CQRSVVIRTF ITSDFMTGVP
660 670 680 690
ATPGNEIPVE VVLKMVTEIK KIPGISRIMY DLTSKPPGTT EWE
Length:693
Mass (Da):76,757
Last modified:July 5, 2005 - v1
Checksum:i03E0AA87B6CDA15D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098016 mRNA. Translation: AAH98016.1.
RefSeqiNP_001019925.1. NM_001024754.1.
UniGeneiRn.12593.

Genome annotation databases

GeneIDi295088.
KEGGirno:295088.
UCSCiRGD:1310063. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098016 mRNA. Translation: AAH98016.1.
RefSeqiNP_001019925.1. NM_001024754.1.
UniGeneiRn.12593.

3D structure databases

ProteinModelPortaliQ4V7C6.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC26.950.

PTM databases

iPTMnetiQ4V7C6.
PhosphoSitePlusiQ4V7C6.

Proteomic databases

PRIDEiQ4V7C6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi295088.
KEGGirno:295088.
UCSCiRGD:1310063. rat.

Organism-specific databases

CTDi8833.
RGDi1310063. Gmps.

Phylogenomic databases

HOGENOMiHOG000223965.
HOVERGENiHBG005929.
InParanoidiQ4V7C6.
KOiK01951.
PhylomeDBiQ4V7C6.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
SABIO-RKQ4V7C6.

Miscellaneous databases

PROiQ4V7C6.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
cd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_GATase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAA_RAT
AccessioniPrimary (citable) accession number: Q4V7C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.