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Protein

WD repeat-containing protein 61

Gene

Wdr61

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-RNO-112382 Formation of RNA Pol II elongation complex
R-RNO-429958 mRNA decay by 3' to 5' exoribonuclease
R-RNO-674695 RNA Polymerase II Pre-transcription Events
R-RNO-75955 RNA Polymerase II Transcription Elongation
R-RNO-8866654 E3 ubiquitin ligases ubiquitinate target proteins

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein 61
Cleaved into the following chain:
Gene namesi
Name:Wdr61
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1308228 Wdr61

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004257501 – 305WD repeat-containing protein 61Add BLAST305
Initiator methionineiRemoved; alternate1 Publication
ChainiPRO_00002458532 – 305WD repeat-containing protein 61, N-terminally processedAdd BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in WD repeat-containing protein 61, N-terminally processed1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ4V7A0
PRIDEiQ4V7A0

Expressioni

Gene expression databases

BgeeiENSRNOG00000012803
ExpressionAtlasiQ4V7A0 baseline and differential
GenevisibleiQ4V7A0 RN

Interactioni

Subunit structurei

Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61 (By similarity). The PAF1 complex interacts with PHF5A. Within the PAF1 complex interacts directly with PHF5A (By similarity). Component of the SKI complex which consists of WDR61, SKIV2L and TTC37 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017239

Structurei

3D structure databases

ProteinModelPortaliQ4V7A0
SMRiQ4V7A0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 57WD 1Add BLAST44
Repeati62 – 101WD 2Add BLAST40
Repeati104 – 143WD 3Add BLAST40
Repeati146 – 187WD 4Add BLAST42
Repeati188 – 227WD 5Add BLAST40
Repeati230 – 269WD 6Add BLAST40
Repeati272 – 305WD 7Add BLAST34

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4155 Eukaryota
ENOG410XP1Z LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000036765
HOVERGENiHBG059813
InParanoidiQ4V7A0
KOiK12602
OMAiERKCLHT
OrthoDBiEOG091G0F6P
PhylomeDBiQ4V7A0

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 6 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4V7A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNQYSILFK QEQAHDDAIW SVAWETNKKE NIETVVTGSL DDLVKVWKWR
60 70 80 90 100
DERLELQWSL EGHQLGVVSV DISHTLPIAA SSSLDAHIRL WDLENGKQMK
110 120 130 140 150
SIDAGPVDAW TLAFSPDSQH LATGTHMGKV NIFGVESGKK EYSLDTRGKF
160 170 180 190 200
ILSIAYSPDG KYLASGAIDG IINIFDIATG KLLHTLEGHA MPIRSLTFSP
210 220 230 240 250
DSQLLVTASD DGYIKIYDVQ HANLAGTLSG HASWVLNVAF CPDDTHFVSS
260 270 280 290 300
SSDKSVKVWD VGTRTCIHTF FDHQDQVWGV KYNGNGSKIV SVGDDQEIHV

YDCPI
Length:305
Mass (Da):33,747
Last modified:July 5, 2005 - v1
Checksum:i02EB895CA3C7E181
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC098059 mRNA Translation: AAH98059.1
RefSeqiNP_001020914.1, NM_001025743.1
XP_006243147.1, XM_006243085.3
XP_006243148.1, XM_006243086.2
UniGeneiRn.3698

Genome annotation databases

EnsembliENSRNOT00000017239; ENSRNOP00000017239; ENSRNOG00000012803
GeneIDi363064
KEGGirno:363064
UCSCiRGD:1308228 rat

Similar proteinsi

Entry informationi

Entry nameiWDR61_RAT
AccessioniPrimary (citable) accession number: Q4V7A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 5, 2005
Last modified: March 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health