Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q4V7A0 (WDR61_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
WD repeat-containing protein 61
Gene names
Name:Wdr61
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length305 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C By similarity.

Subunit structure

Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. Component of the SKI complex which consists of WDR61, SKIV2L and TTC37 By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm By similarity.

Sequence similarities

Contains 7 WD repeats.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
Wnt signaling pathway
   Cellular componentCytoplasm
Nucleus
   DomainRepeat
WD repeat
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

histone H3-K4 trimethylation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of myeloid cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of histone H3-K4 methylation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of histone H3-K79 methylation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription elongation from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentCdc73/Paf1 complex

Inferred from sequence or structural similarity. Source: UniProtKB

Ski complex

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

transcriptionally active chromatin

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 305305WD repeat-containing protein 61
PRO_0000425750
Initiator methionine11Removed; alternate
Chain2 – 305304WD repeat-containing protein 61, N-terminally processed
PRO_0000245853

Regions

Repeat14 – 5744WD 1
Repeat62 – 10140WD 2
Repeat104 – 14340WD 3
Repeat146 – 18742WD 4
Repeat188 – 22740WD 5
Repeat230 – 26940WD 6
Repeat272 – 30534WD 7

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue21N-acetylthreonine; in WD repeat-containing protein 61, N-terminally processed Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q4V7A0 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 02EB895CA3C7E181

FASTA30533,747
        10         20         30         40         50         60 
MTNQYSILFK QEQAHDDAIW SVAWETNKKE NIETVVTGSL DDLVKVWKWR DERLELQWSL 

        70         80         90        100        110        120 
EGHQLGVVSV DISHTLPIAA SSSLDAHIRL WDLENGKQMK SIDAGPVDAW TLAFSPDSQH 

       130        140        150        160        170        180 
LATGTHMGKV NIFGVESGKK EYSLDTRGKF ILSIAYSPDG KYLASGAIDG IINIFDIATG 

       190        200        210        220        230        240 
KLLHTLEGHA MPIRSLTFSP DSQLLVTASD DGYIKIYDVQ HANLAGTLSG HASWVLNVAF 

       250        260        270        280        290        300 
CPDDTHFVSS SSDKSVKVWD VGTRTCIHTF FDHQDQVWGV KYNGNGSKIV SVGDDQEIHV 


YDCPI 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[2]Lubec G., Chen W.-Q.
Submitted (FEB-2007) to UniProtKB
Cited for: ACETYLATION AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC098059 mRNA. Translation: AAH98059.1.
RefSeqNP_001020914.1. NM_001025743.1.
XP_006243147.1. XM_006243085.1.
XP_006243148.1. XM_006243086.1.
UniGeneRn.3698.

3D structure databases

ProteinModelPortalQ4V7A0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000059282.

Proteomic databases

PaxDbQ4V7A0.
PRIDEQ4V7A0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000017239; ENSRNOP00000017239; ENSRNOG00000012803.
GeneID363064.
KEGGrno:363064.
UCSCRGD:1308228. rat.

Organism-specific databases

CTD80349.
RGD1308228. Wdr61.

Phylogenomic databases

eggNOGCOG2319.
GeneTreeENSGT00740000115515.
HOGENOMHOG000036765.
HOVERGENHBG059813.
InParanoidQ4V7A0.
KOK12602.
OMAIATGSHH.
OrthoDBEOG7KM5T5.
PhylomeDBQ4V7A0.

Gene expression databases

GenevestigatorQ4V7A0.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 6 hits.
[Graphical view]
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio682300.

Entry information

Entry nameWDR61_RAT
AccessionPrimary (citable) accession number: Q4V7A0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families