Q4V5A3 (UNPG_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein unplugged

Gene names

Name:	unpg
Synonyms:	unp
ORF Names:	CG1650

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a regulatory role in neural branching of the tracheae: segment-specific aspects of these neural branching patterns appear to be generated by homeotic regulation of expression. May have a role with oc/otd in the postembryonic development of the brain. Ref.1 Ref.5
Subcellular location	Nucleus. Note: In tracheal precursor cells as they migrate and extend. Ref.1
Tissue specificity	Expressed in the neuroectodermal and mesectodermal cells at the ventral midline of stage 8 embryos, Subsequently, expression domains in the CNS widen and have their most anterior border in the posterior deutocerebrum. Oc/otd and unpg are mutual repressors at the interface of their brain-specific expression domains. Expression fades during germ band retraction and is then restricted to subset of cells by stage 14. Expressed in the founder cells of the cerebral branch within the first tracheal metamere. Outside the CNS, expression is seen in two clusters of ectodermal cells located laterally within the labial and first thoracic segments of stage 9 embryos. By stage 13, the expression is detected in a few cells close to the dorsal midline of the embryos. Ref.1 Ref.5
Developmental stage	First detected in 4 hours embryos, expression persists throughout embryogenesis, levels drop during larval stages and then increase again during pupal and adult stages. Ref.1
Disruption phenotype	Embryos display segmentally repeated ganglionic branches that stall and fail to penetrate the CNS and the segment-specific cerebral branch and associated cerebral anastomosis fail to form. Ref.1
Sequence similarities	Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	brain development Inferred from mutant phenotype Ref.5. Source: UniProtKB regulation of nervous system development Inferred from mutant phenotype Ref.1. Source: UniProtKB tracheal outgrowth, open tracheal system Traceable author statement. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Homeobox protein unplugged		PRO_0000299499
Regions
	DNA binding	319 – 378	60	Homeobox
	Compositional bias	111 – 162	52	Pro-rich
Experimental info
	Sequence conflict	107	1	G → A in AAA97404. Ref.1
	Sequence conflict	481	1	A → P in AAA97404. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q4V5A3 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 16B4EF888ADE1D56	FASTA	485	52,230
	10 20 30 40 50 60 MERPALLQNG EIGTMESPTT RLASKPFPKP FSIESLIANQ TPATATPPSP PEERDQEQEA 70 80 90 100 110 120 EQEQELSARA MVASSALGLT QFPLYNPWLH GYFAQNHERL THLIAGGCYL PSSPAGHPAA 130 140 150 160 170 180 QQPQAQAQPQ PPPPHPPTHA LEKQLPPTLP HPLDTRFLPF NPAAAGVAPT DLSYRRLAEL 190 200 210 220 230 240 MNQDYVHSLS VHARLQHMAA AGRMHEDQAN PGMAQLQEPT PPQAHSSPAK SGSHSPMEPA 250 260 270 280 290 300 LDVGMDEDFE CSGDSCSDIS LTMSPRNYNG EMDKSRNGAY TNSDSEDCSD DEGAQSRHEG 310 320 330 340 350 360 GGMGGKDSQG NGSSSNSKSR RRRTAFTSEQ LLELEREFHA KKYLSLTERS QIATSLKLSE 370 380 390 400 410 420 VQVKIWFQNR RAKWKRVKAG LTSHGLGRNG TTSGTKIVVP IPVHVNRFAV RSQHQQLEKM 430 440 450 460 470 480 CLSGPKPDLR KKLSAEAIGG FEKFSGSTNA SSPSGGPVGL GVGVGVGVGV GLGVSTPLSL ARSIY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Control of Drosophila tracheal branching by the novel homeodomain gene unplugged, a regulatory target for genes of the bithorax complex." Chiang C., Young K.E., Beachy P.A. Development 121:3901-3912(1995) [PubMed: 8582298] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
[5]	"An urbilaterian origin of the tripartite brain: developmental genetic insights from Drosophila." Hirth F., Kammermeier L., Frei E., Walldorf U., Noll M., Reichert H. Development 130:2365-2373(2003) [PubMed: 12702651] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U35427 mRNA. Translation: AAA97404.1. AE013599 Genomic DNA. Translation: AAF58968.1. BT022753 mRNA. Translation: AAY55169.1.
RefSeq	NP_477146.1. NM_057798.2.
UniGene	Dm.6807.
3D structure databases
ProteinModelPortal	Q4V5A3.
SMR	Q4V5A3. Positions 274-378.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4V5A3.
Proteomic databases
PRIDE	Q4V5A3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0088586; FBpp0087667; FBgn0015561.
GeneID	35942.
KEGG	dme:Dmel_CG1650.
NMPDR	fig|7227.3.peg.4041.
UCSC	CG1650-RA. d. melanogaster.
Organism-specific databases
CTD	35942.
FlyBase	FBgn0015561. unpg.
Phylogenomic databases
eggNOG	inNOG10489.
GeneTree	EMGT00070000025675.
InParanoid	Q4V5A3.
OMA	FAQNHER.
OrthoDB	EOG4H70T3.
PhylomeDB	Q4V5A3.
Gene expression databases
Bgee	Q4V5A3.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR020479. Homeobox_eu. IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K09321.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	795976.

Entry information

Entry name

UNPG_DROME

Accession

Primary (citable) accession number: Q4V5A3
Secondary accession number(s): Q24136

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 3, 2006
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project