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Q4V3B3

- BGL28_ARATH

UniProt

Q4V3B3 - BGL28_ARATH

Protein

Beta-glucosidase 28

Gene

BGLU28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei48 – 481SubstrateBy similarity
    Binding sitei151 – 1511SubstrateBy similarity
    Binding sitei196 – 1961SubstrateBy similarity
    Active sitei197 – 1971Proton donorBy similarity
    Binding sitei340 – 3401SubstrateBy similarity
    Active sitei412 – 4121NucleophileBy similarity
    Binding sitei462 – 4621SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. response to karrikin Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT2G44460-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 28 (EC:3.2.1.21)
    Short name:
    AtBGLU28
    Gene namesi
    Name:BGLU28
    Ordered Locus Names:At2g44460
    ORF Names:F4I1.27
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G44460.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 582561Beta-glucosidase 28PRO_0000389590Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi216 ↔ 224By similarity
    Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi521 – 5211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ4V3B3.

    Expressioni

    Gene expression databases

    GenevestigatoriQ4V3B3.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G44460.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4V3B3.
    SMRiQ4V3B3. Positions 29-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni469 – 4702Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiQ4V3B3.
    KOiK01188.
    OMAiIGRREAP.
    PhylomeDBiQ4V3B3.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q4V3B3-1 [UniParc]FASTAAdd to Basket

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    MKMHFFILLV ITSWLSEKIT SLPPDSRVFD RHGFPDNFVF GTAASAFQYE    50
    GATSEGGKSP SIWDYFSHTF PERTRMQNAD VAVDFYHRYK DDIKLMKELN 100
    MDAFRFSISW ARLIPSGKVK DGVNKEGVEF YKALIDELVA NGIEPSMTLY 150
    HWDHPQSLED EYGGFLSPQI VEDFRDFSRV CFEEFGDKVK MWTTINEPYV 200
    ITVAGYDTGN KAVGRCSKWV NSKCQGGDSG TEPYIASHHL LLAHAAAVQE 250
    FRKCNKTQDG QIGIVLSPLW FEPYDSASPA DNEAVKRALA TELDWHLDPV 300
    IHGDYPEMMK KLAGNRLPSF TPEQSKMLKN SSDFIGINYY TARYVAHIPQ 350
    ADPARPRFVT DHQLQWRVTN HSNHQFGPGE DRGILQSHPE GLRKVLNYIK 400
    DKYNNPIVYI KENGINDYDD GTKSREEILN DTFRISYHED HLQQLQKAII 450
    EDGCDVRGYY VWSLLDNFEW EHGYSTRFGV YYVDYDNDLT RIPKDSVNWF 500
    KQFLDVKNKE IWDVSHKERY NKTFDDVESF EASVGSILYL MTNNISRREE 550
    EERDQCAFGN LNDQSGLLLE SYNSFGFLEN VW 582
    Length:582
    Mass (Da):67,299
    Last modified:July 5, 2005 - v1
    Checksum:i6F551146E571F180
    GO

    Sequence cautioni

    The sequence AAC16092.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004521 Genomic DNA. Translation: AAC16092.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC10422.1.
    BT023443 mRNA. Translation: AAY56434.1.
    PIRiT02401.
    RefSeqiNP_850416.1. NM_180085.3.
    UniGeneiAt.36757.

    Genome annotation databases

    EnsemblPlantsiAT2G44460.1; AT2G44460.1; AT2G44460.
    GeneIDi819053.
    KEGGiath:AT2G44460.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004521 Genomic DNA. Translation: AAC16092.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC10422.1 .
    BT023443 mRNA. Translation: AAY56434.1 .
    PIRi T02401.
    RefSeqi NP_850416.1. NM_180085.3.
    UniGenei At.36757.

    3D structure databases

    ProteinModelPortali Q4V3B3.
    SMRi Q4V3B3. Positions 29-504.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G44460.1-P.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PRIDEi Q4V3B3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G44460.1 ; AT2G44460.1 ; AT2G44460 .
    GeneIDi 819053.
    KEGGi ath:AT2G44460.

    Organism-specific databases

    TAIRi AT2G44460.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    InParanoidi Q4V3B3.
    KOi K01188.
    OMAi IGRREAP.
    PhylomeDBi Q4V3B3.

    Enzyme and pathway databases

    BioCyci ARA:AT2G44460-MONOMER.

    Gene expression databases

    Genevestigatori Q4V3B3.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Arabidopsis ORF clones."
      Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGL28_ARATH
    AccessioniPrimary (citable) accession number: Q4V3B3
    Secondary accession number(s): O64880
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3