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Q4V3B3

- BGL28_ARATH

UniProt

Q4V3B3 - BGL28_ARATH

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Protein

Beta-glucosidase 28

Gene

BGLU28

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481SubstrateBy similarity
Binding sitei151 – 1511SubstrateBy similarity
Binding sitei196 – 1961SubstrateBy similarity
Active sitei197 – 1971Proton donorBy similarity
Binding sitei340 – 3401SubstrateBy similarity
Active sitei412 – 4121NucleophileBy similarity
Binding sitei462 – 4621SubstrateBy similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. response to karrikin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G44460-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 28 (EC:3.2.1.21)
Short name:
AtBGLU28
Gene namesi
Name:BGLU28
Ordered Locus Names:At2g44460
ORF Names:F4I1.27
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G44460.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 582561Beta-glucosidase 28PRO_0000389590Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi216 ↔ 224By similarity
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi330 – 3301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi370 – 3701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi521 – 5211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi544 – 5441N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ4V3B3.

Expressioni

Gene expression databases

GenevestigatoriQ4V3B3.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G44460.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ4V3B3.
SMRiQ4V3B3. Positions 29-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni469 – 4702Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ4V3B3.
KOiK01188.
OMAiIGRREAP.
PhylomeDBiQ4V3B3.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4V3B3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMHFFILLV ITSWLSEKIT SLPPDSRVFD RHGFPDNFVF GTAASAFQYE
60 70 80 90 100
GATSEGGKSP SIWDYFSHTF PERTRMQNAD VAVDFYHRYK DDIKLMKELN
110 120 130 140 150
MDAFRFSISW ARLIPSGKVK DGVNKEGVEF YKALIDELVA NGIEPSMTLY
160 170 180 190 200
HWDHPQSLED EYGGFLSPQI VEDFRDFSRV CFEEFGDKVK MWTTINEPYV
210 220 230 240 250
ITVAGYDTGN KAVGRCSKWV NSKCQGGDSG TEPYIASHHL LLAHAAAVQE
260 270 280 290 300
FRKCNKTQDG QIGIVLSPLW FEPYDSASPA DNEAVKRALA TELDWHLDPV
310 320 330 340 350
IHGDYPEMMK KLAGNRLPSF TPEQSKMLKN SSDFIGINYY TARYVAHIPQ
360 370 380 390 400
ADPARPRFVT DHQLQWRVTN HSNHQFGPGE DRGILQSHPE GLRKVLNYIK
410 420 430 440 450
DKYNNPIVYI KENGINDYDD GTKSREEILN DTFRISYHED HLQQLQKAII
460 470 480 490 500
EDGCDVRGYY VWSLLDNFEW EHGYSTRFGV YYVDYDNDLT RIPKDSVNWF
510 520 530 540 550
KQFLDVKNKE IWDVSHKERY NKTFDDVESF EASVGSILYL MTNNISRREE
560 570 580
EERDQCAFGN LNDQSGLLLE SYNSFGFLEN VW
Length:582
Mass (Da):67,299
Last modified:July 5, 2005 - v1
Checksum:i6F551146E571F180
GO

Sequence cautioni

The sequence AAC16092.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004521 Genomic DNA. Translation: AAC16092.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10422.1.
BT023443 mRNA. Translation: AAY56434.1.
PIRiT02401.
RefSeqiNP_850416.1. NM_180085.3.
UniGeneiAt.36757.

Genome annotation databases

EnsemblPlantsiAT2G44460.1; AT2G44460.1; AT2G44460.
GeneIDi819053.
KEGGiath:AT2G44460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004521 Genomic DNA. Translation: AAC16092.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC10422.1 .
BT023443 mRNA. Translation: AAY56434.1 .
PIRi T02401.
RefSeqi NP_850416.1. NM_180085.3.
UniGenei At.36757.

3D structure databases

ProteinModelPortali Q4V3B3.
SMRi Q4V3B3. Positions 29-504.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G44460.1-P.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEi Q4V3B3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G44460.1 ; AT2G44460.1 ; AT2G44460 .
GeneIDi 819053.
KEGGi ath:AT2G44460.

Organism-specific databases

TAIRi AT2G44460.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi Q4V3B3.
KOi K01188.
OMAi IGRREAP.
PhylomeDBi Q4V3B3.

Enzyme and pathway databases

BioCyci ARA:AT2G44460-MONOMER.

Gene expression databases

Genevestigatori Q4V3B3.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL28_ARATH
AccessioniPrimary (citable) accession number: Q4V3B3
Secondary accession number(s): O64880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: July 5, 2005
Last modified: October 1, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3