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Q4V3B3

- BGL28_ARATH

UniProt

Q4V3B3 - BGL28_ARATH

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Protein
Beta-glucosidase 28
Gene
BGLU28, At2g44460, F4I1.27
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481Substrate By similarity
Binding sitei151 – 1511Substrate By similarity
Binding sitei196 – 1961Substrate By similarity
Active sitei197 – 1971Proton donor By similarity
Binding sitei340 – 3401Substrate By similarity
Active sitei412 – 4121Nucleophile By similarity
Binding sitei462 – 4621Substrate By similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. response to karrikin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G44460-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 28 (EC:3.2.1.21)
Short name:
AtBGLU28
Gene namesi
Name:BGLU28
Ordered Locus Names:At2g44460
ORF Names:F4I1.27
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G44460.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed prediction
Add
BLAST
Chaini22 – 582561Beta-glucosidase 28
PRO_0000389590Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi216 ↔ 224 By similarity
Glycosylationi255 – 2551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi330 – 3301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi370 – 3701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi430 – 4301N-linked (GlcNAc...) Reviewed prediction
Glycosylationi521 – 5211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi544 – 5441N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ4V3B3.

Expressioni

Gene expression databases

GenevestigatoriQ4V3B3.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G44460.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ4V3B3.
SMRiQ4V3B3. Positions 29-504.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni469 – 4702Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ4V3B3.
KOiK01188.
OMAiIGRREAP.
PhylomeDBiQ4V3B3.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4V3B3-1 [UniParc]FASTAAdd to Basket

« Hide

MKMHFFILLV ITSWLSEKIT SLPPDSRVFD RHGFPDNFVF GTAASAFQYE    50
GATSEGGKSP SIWDYFSHTF PERTRMQNAD VAVDFYHRYK DDIKLMKELN 100
MDAFRFSISW ARLIPSGKVK DGVNKEGVEF YKALIDELVA NGIEPSMTLY 150
HWDHPQSLED EYGGFLSPQI VEDFRDFSRV CFEEFGDKVK MWTTINEPYV 200
ITVAGYDTGN KAVGRCSKWV NSKCQGGDSG TEPYIASHHL LLAHAAAVQE 250
FRKCNKTQDG QIGIVLSPLW FEPYDSASPA DNEAVKRALA TELDWHLDPV 300
IHGDYPEMMK KLAGNRLPSF TPEQSKMLKN SSDFIGINYY TARYVAHIPQ 350
ADPARPRFVT DHQLQWRVTN HSNHQFGPGE DRGILQSHPE GLRKVLNYIK 400
DKYNNPIVYI KENGINDYDD GTKSREEILN DTFRISYHED HLQQLQKAII 450
EDGCDVRGYY VWSLLDNFEW EHGYSTRFGV YYVDYDNDLT RIPKDSVNWF 500
KQFLDVKNKE IWDVSHKERY NKTFDDVESF EASVGSILYL MTNNISRREE 550
EERDQCAFGN LNDQSGLLLE SYNSFGFLEN VW 582
Length:582
Mass (Da):67,299
Last modified:July 5, 2005 - v1
Checksum:i6F551146E571F180
GO

Sequence cautioni

The sequence AAC16092.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004521 Genomic DNA. Translation: AAC16092.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC10422.1.
BT023443 mRNA. Translation: AAY56434.1.
PIRiT02401.
RefSeqiNP_850416.1. NM_180085.3.
UniGeneiAt.36757.

Genome annotation databases

EnsemblPlantsiAT2G44460.1; AT2G44460.1; AT2G44460.
GeneIDi819053.
KEGGiath:AT2G44460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004521 Genomic DNA. Translation: AAC16092.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC10422.1 .
BT023443 mRNA. Translation: AAY56434.1 .
PIRi T02401.
RefSeqi NP_850416.1. NM_180085.3.
UniGenei At.36757.

3D structure databases

ProteinModelPortali Q4V3B3.
SMRi Q4V3B3. Positions 29-504.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G44460.1-P.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEi Q4V3B3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G44460.1 ; AT2G44460.1 ; AT2G44460 .
GeneIDi 819053.
KEGGi ath:AT2G44460.

Organism-specific databases

TAIRi AT2G44460.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi Q4V3B3.
KOi K01188.
OMAi IGRREAP.
PhylomeDBi Q4V3B3.

Enzyme and pathway databases

BioCyci ARA:AT2G44460-MONOMER.

Gene expression databases

Genevestigatori Q4V3B3.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL28_ARATH
AccessioniPrimary (citable) accession number: Q4V3B3
Secondary accession number(s): O64880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi