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Reviewed, UniProtKB/Swiss-Prot Q4V328 (GRAP1_HUMAN)

Last modified January 19, 2010. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    GRIP1-associated protein 1
      Short name=GRASP-1
Gene names
Name: GRIPAP1
Synonyms: KIAA1167
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length841 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Subunit structure

Interacts with GRIP1, GRIP2 and AMPA receptors By similarity.

Subcellular location

Early endosome Ref.7.

Post-translational modification

Proteolytically cleaved by caspase-3 By similarity.

Involvement in disease

Antibodies against GRIPAP1 have been found in sera of a patient who developed Raynaud's syndrome and telangiectasias. Ref.7

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Ontologies

Keywords
   Cellular componentEndosome
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Cellular componentearly endosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q4V328-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q4V328-2)

The sequence of this isoform differs from the canonical sequence as follows:
     103-155: Missing.
     534-559: Missing.
     688-704: SLSSSPQAQPPRPAELS → VRGKEEPTAPASLNPKI
     705-841: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q4V328-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-110: Missing.
     111-152: EQENQQLKEG...ENTALQKNVA → MWSFGTGTLP...IHHIFLFYSP
     688-704: SLSSSPQAQPPRPAELS → VRGKEEPTAPASLNPKI
     705-841: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q4V328-4)

The sequence of this isoform differs from the canonical sequence as follows:
     317-347: Missing.
Note: No experimental confirmation available.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 841841GRIP1-associated protein 1
PRO_0000087581

Regions

Coiled coil4 – 161158 Potential
Coiled coil208 – 641434 Potential
Coiled coil701 – 73535 Potential
Coiled coil785 – 81430 Potential

Sites

Site598 – 5992Cleavage; by caspase-3 By similarity

Amino acid modifications

Modified residue6551Phosphoserine Ref.8 Ref.9
Modified residue6661Phosphoserine Ref.11
Modified residue6691Phosphoserine By similarity
Modified residue6901Phosphoserine Ref.11
Modified residue6921Phosphoserine Ref.11

Natural variations

Alternative sequence1 – 110110Missing in isoform 3.
VSP_015699
Alternative sequence103 – 15553Missing in isoform 2.
VSP_015700
Alternative sequence111 – 15242EQENQ…QKNVA → MWSFGTGTLPPEGLKASSPS IRGMARDFRDHLIHHIFLFY SP in isoform 3.
VSP_015701
Alternative sequence317 – 34731Missing in isoform 4.
VSP_015702
Alternative sequence534 – 55926Missing in isoform 2.
VSP_015703
Alternative sequence688 – 70417SLSSS…PAELS → VRGKEEPTAPASLNPKI in isoform 2 and isoform 3.
VSP_015704
Alternative sequence705 – 841137Missing in isoform 2 and isoform 3.
VSP_015705

Experimental info

Sequence conflict6771R → G in CAB95949. Ref.6

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 48877D04F6630213

FASTA84195,990
        10         20         30         40         50         60 
MAQALSEEEF QRMQAQLLEL RTNNYQLSDE LRKNGVELTS LRQKVAYLDK EFSKAQKALS 

        70         80         90        100        110        120 
KSKKAQEVEV LLSENEMLQA KLHSQEEDFR LQNSTLMAEF SKLCSQMEQL EQENQQLKEG 

       130        140        150        160        170        180 
AAGAGVAQAG PLVDGELLRL QAENTALQKN VAALQERYGK EAGKFSAVSE GQGDPPGGPA 

       190        200        210        220        230        240 
PTVLAPMPLA EVELKWEMEK EEKRLLWEQL QGLESSKQAE TSRLQEELAK LSEKLKKKQE 

       250        260        270        280        290        300 
SFCRLQTEKE TLFNDSRNKI EELQQRKEAD HKAQLARTQK LQQELEAANQ SLAELRDQRQ 

       310        320        330        340        350        360 
GERLEHAAAL RALQDQVSIQ SADAQEQVEG LLAENNALRT SLAALEQIQT AKTQELNMLR 

       370        380        390        400        410        420 
EQTTGLAAEL QQQQAEYEDL MGQKDDLNSQ LQESLRANSR LLEQLQEIGQ EKEQLTQELQ 

       430        440        450        460        470        480 
EARKSAEKRK AMLDELAMET LQEKSQHKEE LGAVRLRHEK EVLGVRARYE RELRELHEDK 

       490        500        510        520        530        540 
KRQEEELRGQ IREEKARTRE LETLQQTVEE LQAQVHSMDG AKGWFERRLK EAEESLQQQQ 

       550        560        570        580        590        600 
QEQEEALKQC REQHAAELKG KEEELQDVRD QLEQAQEERD CHLKTISSLK QEVKDTVDGQ 

       610        620        630        640        650        660 
RILEKKGSAA LKDLKRQLHL ERKRADKLQE RLQDILTNSK SRSGLEELVL SEMNSPSRTQ 

       670        680        690        700        710        720 
TGDSSSISSF SYREILREKE SSAVPARSLS SSPQAQPPRP AELSDEEVAE LFQRLAETQQ 

       730        740        750        760        770        780 
EKWMLEEKVK HLEVSSASMA EDLCRKSAII ETYVMDSRID VSVAAGHTDR SGLGSVLRDL 

       790        800        810        820        830        840 
VKPGDENLRE MNKKLQNMLE EQLTKNMHLH KDMEVLSQEI VRLSKECVGP PDPDLEPGET 


S

« Hide

Isoform 2.

Checksum: C79355243210C480
Show »

FASTA62571,878
Isoform 3.

Checksum: 51E9C3CE7EA5B184
Show »

FASTA59468,188
Isoform 4.

Checksum: BC469E1FADB93F74
Show »

FASTA81092,738

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References

« Hide 'large scale' references
[1]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Prostate and Retina.
[2]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed: 15772651] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 4).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Muscle.
[4]"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain."
Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.
DNA Res. 6:329-336(1999) [PubMed: 10574461] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-841 (ISOFORM 1).
Tissue: Brain.
[5]Lubec G., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 401-412, MASS SPECTROMETRY.
Tissue: Fetal brain cortex.
[6]"By-passing selection: direct screening for antibody-antigen interactions using protein arrays."
Holt L.J., Bussow K., Walter G., Tomlinson I.M.
Nucleic Acids Res. 28:72-72(2000) [PubMed: 10908365] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 677-841.
Tissue: Fetal brain.
[7]"Identification of GRASP-1 as a novel 97 kDa autoantigen localized to endosomes."
Stinton L.M., Selak S., Fritzler M.J.
Clin. Immunol. 116:108-117(2005) [PubMed: 15897011] [Abstract]
Cited for: SUBCELLULAR LOCATION, DISEASE.
[8]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, MASS SPECTROMETRY.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, MASS SPECTROMETRY.
[10]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-666; SER-690 AND SER-692, MASS SPECTROMETRY.
Tissue: T-cell.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX640704 mRNA. Translation: CAE45824.1.
BX647804 mRNA. Translation: CAI45985.1. Different termination.
BX530088, AF207550 Genomic DNA. Translation: CAI95784.1.
BX530088, AF207550 Genomic DNA. Translation: CAI95786.1.
BX530088, AF207550 Genomic DNA. Translation: CAI95787.1.
BC001522 mRNA. Translation: AAH01522.2.
BC101544 mRNA. Translation: AAI01545.1.
BC101546 mRNA. Translation: AAI01547.1.
AB032993 mRNA. Translation: BAA86481.1.
AJ297364 mRNA. Translation: CAB95949.1.
IPIIPI00414215.
IPI00423384.
IPI00607628.
IPI00873904.
RefSeqNP_064522.3.
NP_997555.1.
UniGeneHs.109929

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ4V328. 1 interaction.
STRINGQ4V328.

PTM databases

PhosphoSiteQ4V328.

Proteomic databases

PRIDEQ4V328.

Genome annotation databases

EnsemblENST00000376441; ENSP00000365624; ENSG00000068400; Homo sapiens. [Genome view]
GeneID56850.
KEGGhsa:56850.
UCSCuc004dly.1. human.
uc004dlz.1. human.
uc004dma.1. human.

Organism-specific databases

CTD56850.
GeneCardsGC0XM048715.
H-InvDBHIX0022795.
HGNCHGNC:18706. GRIPAP1.
HPAHPA000282.
HPA000615.
MIM300408. gene.
PharmGKBPA38650.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG08639.
HOVERGENQ4V328.
OMANMHLHKD.
OrthoDBEOG9CG2VS.

Gene expression databases

ArrayExpressQ4V328.
BgeeQ4V328.
GenevestigatorQ4V328.
GermOnlineENSG00000068400. Homo sapiens.

Family and domain databases

ProtoNetSearch...

Other Resources

NextBio62252.
PMAP-CutDBQ4V328.
SOURCESearch...

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Entry information

Entry nameGRAP1_HUMAN
AccessionPrimary (citable) accession number: Q4V328
Secondary accession number(s): A6NL78 expand/collapse secondary AC list , Q3MJ75, Q4V327, Q4V330, Q5HYG1, Q6N046, Q96DH8, Q9NQ43, Q9ULQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 5, 2005
Last modified: January 19, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Relevant documents

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents