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Q4V328 (GRAP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GRIP1-associated protein 1

Short name=GRASP-1
Gene names
Name:GRIPAP1
Synonyms:KIAA1167
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length841 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Interacts with GRIP1, GRIP2 and AMPA receptors By similarity.

Subcellular location

Early endosome Ref.7.

Post-translational modification

Proteolytically cleaved by caspase-3 By similarity.

Miscellaneous

Antibodies against GRIPAP1 have been found in sera of a patient who developed Raynaud's syndrome and telangiectasias.

Ontologies

Keywords
   Cellular componentEndosome
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentblood microparticle

Inferred from direct assay PubMed 22516433. Source: UniProt

early endosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q4V328-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q4V328-2)

The sequence of this isoform differs from the canonical sequence as follows:
     103-155: Missing.
     534-559: Missing.
     688-704: SLSSSPQAQPPRPAELS → VRGKEEPTAPASLNPKI
     705-841: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q4V328-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-110: Missing.
     111-152: EQENQQLKEG...ENTALQKNVA → MWSFGTGTLP...IHHIFLFYSP
     688-704: SLSSSPQAQPPRPAELS → VRGKEEPTAPASLNPKI
     705-841: Missing.
Note: No experimental confirmation available.
Isoform 4 (identifier: Q4V328-4)

The sequence of this isoform differs from the canonical sequence as follows:
     317-347: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.15
Chain2 – 841840GRIP1-associated protein 1
PRO_0000087581

Regions

Coiled coil4 – 161158 Potential
Coiled coil208 – 641434 Potential
Coiled coil701 – 73535 Potential
Coiled coil785 – 81430 Potential

Sites

Site598 – 5992Cleavage; by caspase-3 By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.15 Ref.16
Modified residue6551Phosphoserine Ref.10 Ref.12
Modified residue6661Phosphoserine Ref.11
Modified residue6691Phosphoserine By similarity
Modified residue6901Phosphoserine Ref.11
Modified residue6921Phosphoserine Ref.11

Natural variations

Alternative sequence1 – 110110Missing in isoform 3.
VSP_015699
Alternative sequence103 – 15553Missing in isoform 2.
VSP_015700
Alternative sequence111 – 15242EQENQ…QKNVA → MWSFGTGTLPPEGLKASSPS IRGMARDFRDHLIHHIFLFY SP in isoform 3.
VSP_015701
Alternative sequence317 – 34731Missing in isoform 4.
VSP_015702
Alternative sequence534 – 55926Missing in isoform 2.
VSP_015703
Alternative sequence688 – 70417SLSSS…PAELS → VRGKEEPTAPASLNPKI in isoform 2 and isoform 3.
VSP_015704
Alternative sequence705 – 841137Missing in isoform 2 and isoform 3.
VSP_015705

Experimental info

Sequence conflict6771R → G in CAB95949. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 48877D04F6630213

FASTA84195,990
        10         20         30         40         50         60 
MAQALSEEEF QRMQAQLLEL RTNNYQLSDE LRKNGVELTS LRQKVAYLDK EFSKAQKALS 

        70         80         90        100        110        120 
KSKKAQEVEV LLSENEMLQA KLHSQEEDFR LQNSTLMAEF SKLCSQMEQL EQENQQLKEG 

       130        140        150        160        170        180 
AAGAGVAQAG PLVDGELLRL QAENTALQKN VAALQERYGK EAGKFSAVSE GQGDPPGGPA 

       190        200        210        220        230        240 
PTVLAPMPLA EVELKWEMEK EEKRLLWEQL QGLESSKQAE TSRLQEELAK LSEKLKKKQE 

       250        260        270        280        290        300 
SFCRLQTEKE TLFNDSRNKI EELQQRKEAD HKAQLARTQK LQQELEAANQ SLAELRDQRQ 

       310        320        330        340        350        360 
GERLEHAAAL RALQDQVSIQ SADAQEQVEG LLAENNALRT SLAALEQIQT AKTQELNMLR 

       370        380        390        400        410        420 
EQTTGLAAEL QQQQAEYEDL MGQKDDLNSQ LQESLRANSR LLEQLQEIGQ EKEQLTQELQ 

       430        440        450        460        470        480 
EARKSAEKRK AMLDELAMET LQEKSQHKEE LGAVRLRHEK EVLGVRARYE RELRELHEDK 

       490        500        510        520        530        540 
KRQEEELRGQ IREEKARTRE LETLQQTVEE LQAQVHSMDG AKGWFERRLK EAEESLQQQQ 

       550        560        570        580        590        600 
QEQEEALKQC REQHAAELKG KEEELQDVRD QLEQAQEERD CHLKTISSLK QEVKDTVDGQ 

       610        620        630        640        650        660 
RILEKKGSAA LKDLKRQLHL ERKRADKLQE RLQDILTNSK SRSGLEELVL SEMNSPSRTQ 

       670        680        690        700        710        720 
TGDSSSISSF SYREILREKE SSAVPARSLS SSPQAQPPRP AELSDEEVAE LFQRLAETQQ 

       730        740        750        760        770        780 
EKWMLEEKVK HLEVSSASMA EDLCRKSAII ETYVMDSRID VSVAAGHTDR SGLGSVLRDL 

       790        800        810        820        830        840 
VKPGDENLRE MNKKLQNMLE EQLTKNMHLH KDMEVLSQEI VRLSKECVGP PDPDLEPGET 


S 

« Hide

Isoform 2 [UniParc].

Checksum: C79355243210C480
Show »

FASTA62571,878
Isoform 3 [UniParc].

Checksum: 51E9C3CE7EA5B184
Show »

FASTA59468,188
Isoform 4 [UniParc].

Checksum: BC469E1FADB93F74
Show »

FASTA81092,738

References

« Hide 'large scale' references
[1]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Prostate and Retina.
[2]"The DNA sequence of the human X chromosome."
Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. expand/collapse author list , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R.
Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 4).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Muscle.
[4]"Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain."
Hirosawa M., Nagase T., Ishikawa K., Kikuno R., Nomura N., Ohara O.
DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 8-841 (ISOFORM 1).
Tissue: Brain.
[5]Lubec G., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 401-412, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Fetal brain cortex.
[6]"By-passing selection: direct screening for antibody-antigen interactions using protein arrays."
Holt L.J., Bussow K., Walter G., Tomlinson I.M.
Nucleic Acids Res. 28:72-72(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 677-841.
Tissue: Fetal brain.
[7]"Identification of GRASP-1 as a novel 97 kDa autoantigen localized to endosomes."
Stinton L.M., Selak S., Fritzler M.J.
Clin. Immunol. 116:108-117(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, ROLE IN RAYNAUD SYNDROME.
[8]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-666; SER-690 AND SER-692, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[16]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX640704 mRNA. Translation: CAE45824.1.
BX647804 mRNA. Translation: CAI45985.1. Different termination.
BX530088, AF207550 Genomic DNA. Translation: CAI95784.1.
BX530088, AF207550 Genomic DNA. Translation: CAI95786.1.
BX530088, AF207550 Genomic DNA. Translation: CAI95787.1.
BC001522 mRNA. Translation: AAH01522.2.
BC101544 mRNA. Translation: AAI01545.1.
BC101546 mRNA. Translation: AAI01547.1.
AB032993 mRNA. Translation: BAA86481.1.
AJ297364 mRNA. Translation: CAB95949.1.
CCDSCCDS35248.1. [Q4V328-1]
RefSeqNP_064522.3. NM_020137.4. [Q4V328-1]
UniGeneHs.109929.

3D structure databases

ProteinModelPortalQ4V328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121210. 10 interactions.
DIPDIP-47299N.
IntActQ4V328. 3 interactions.
MINTMINT-1395880.

PTM databases

PhosphoSiteQ4V328.

Polymorphism databases

DMDM74753569.

Proteomic databases

MaxQBQ4V328.
PaxDbQ4V328.
PRIDEQ4V328.

Protocols and materials databases

DNASU56850.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000376423; ENSP00000365606; ENSG00000068400. [Q4V328-2]
ENST00000376425; ENSP00000365608; ENSG00000068400. [Q4V328-4]
ENST00000376441; ENSP00000365624; ENSG00000068400. [Q4V328-1]
GeneID56850.
KEGGhsa:56850.
UCSCuc004dly.1. human. [Q4V328-1]
uc004dlz.3. human. [Q4V328-3]
uc004dma.3. human. [Q4V328-2]

Organism-specific databases

CTD56850.
GeneCardsGC0XM048830.
HGNCHGNC:18706. GRIPAP1.
HPAHPA000282.
HPA000615.
MIM300408. gene.
neXtProtNX_Q4V328.
PharmGKBPA38650.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG135471.
HOVERGENHBG080243.
OMATIETENS.
OrthoDBEOG7BCNB6.
PhylomeDBQ4V328.
TreeFamTF329006.

Gene expression databases

ArrayExpressQ4V328.
BgeeQ4V328.
GenevestigatorQ4V328.

Family and domain databases

InterProIPR026204. GRIPAP1.
[Graphical view]
PANTHERPTHR18978. PTHR18978. 1 hit.
ProtoNetSearch...

Other

GeneWikiGRIPAP1.
GenomeRNAi56850.
NextBio62252.
PMAP-CutDBQ4V328.
PROQ4V328.
SOURCESearch...

Entry information

Entry nameGRAP1_HUMAN
AccessionPrimary (citable) accession number: Q4V328
Secondary accession number(s): A6NL78 expand/collapse secondary AC list , Q3MJ75, Q4V327, Q4V330, Q5HYG1, Q6N046, Q96DH8, Q9NQ43, Q9ULQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: July 5, 2005
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome X

Human chromosome X: entries, gene names and cross-references to MIM