Q4UZN8 (BIOB_XANC8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	XC_0400

Organism

Xanthomonas campestris pv. campestris (strain 8004) [Complete proteome] [HAMAP]

Taxonomic identifier

314565 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas › Xanthomonas campestris › Xanthomonas campestris pv. campestris

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381708
Sites
	Metal binding	55	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	62	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	99	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	130	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	190	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	262	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UZN8 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 4C576AF23C7F7D76	FASTA	344	37,516
	10 20 30 40 50 60 MSVVVRHDWD RKELHALFAL PFPELLHRAA SVHRAHFDPA EVQVSTLLSV KTGGCPEDCA 70 80 90 100 110 120 YCPQAQRYDT GVSAQKLMDT EDVVAKARQA KAAGASRFCM GAAWRSPKDR DIPKVAAMIR 130 140 150 160 170 180 EVKAMGLETC ATLGMLDAGQ ARALKDAGLD YYNHNLDTAP DYYDSIIHTR QYQDRLNTLE 190 200 210 220 230 240 HVRDVGLKTC CGGIVGMGET REHRVGLLHA LATLPAHPDS VPINQLVQVP GTPLHGTEPL 250 260 270 280 290 300 DAFEFVRMIA VARIAMPKSM VRLSAGREAM SDELQALCFL AGANSIFYGE KLLTTGNPDT 310 320 330 340 ERDQGLFQRL GLRPMQITVD AAEHDHPGTV HADITCGAAC EHAA

References

[1]

"Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris." Qian W., Jia Y., Ren S.-X., He Y.-Q., Feng J.-X., Lu L.-F., Sun Q., Ying G., Tang D.-J., Tang H., Wu W., Hao P., Wang L., Jiang B.-L., Zeng S., Gu W.-Y., Lu G., Rong L. , Tian Y., Yao Z., Fu G., Chen B., Fang R., Qiang B., Chen Z., Zhao G.-P., Tang J.-L., He C. Genome Res. 15:757-767(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 8004.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000050 Genomic DNA. Translation: AAY47485.1.
RefSeq	YP_241505.1. NC_007086.1.
3D structure databases
ProteinModelPortal	Q4UZN8.
SMR	Q4UZN8. Positions 6-316.
ModBase	Search...
Protein-protein interaction databases
STRING	314565.XC_0400.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY47485; AAY47485; XC_0400.
GeneID	3381597.
KEGG	xcb:XC_0400.
PATRIC	24062028. VBIXanCam24967_0426.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239957.
KO	K01012.
OMA	IACCGRA.
ProtClustDB	CLSK2300892.
Enzyme and pathway databases
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_XANC8

Accession

Primary (citable) accession number: Q4UZN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program