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Q4UZF6

- CLP_XANC8

UniProt

Q4UZF6 - CLP_XANC8

Protein

CRP-like protein Clp

Gene

clp

Organism
Xanthomonas campestris pv. campestris (strain 8004)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Global transcriptional regulator that regulates virulence factors production by activating or repressing the expression of a large set of genes in diffusible signal factor (DSF) pathway.1 Publication

    Enzyme regulationi

    Allosterically inhibited by cyclic di-GMP (c-di-GMP), which binds to Clp and abolishes its ability to bind its target gene promoter.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi18 – 139122cNMPAdd
    BLAST
    DNA bindingi190 – 20920H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. catalytic activity Source: UniProtKB-KW
    2. cyclic-di-GMP binding Source: UniProtKB
    3. DNA binding Source: UniProtKB
    4. protein dimerization activity Source: UniProtKB
    5. sequence-specific DNA binding transcription factor activity Source: UniProtKB

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: UniProtKB
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation, Virulence

    Keywords - Ligandi

    c-di-GMP, DNA-binding

    Enzyme and pathway databases

    BioCyciXCAM314565:GCQG-483-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CRP-like protein Clp
    Alternative name(s):
    Catabolite activation-like protein
    Short name:
    CAP-like
    Gene namesi
    Name:clp
    Ordered Locus Names:XC_0486
    OrganismiXanthomonas campestris pv. campestris (strain 8004)
    Taxonomic identifieri314565 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
    ProteomesiUP000000420: Chromosome

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi99 – 991E → S: No change in DNA-binding, but decrease in response to c-di-GMP. 1 Publication
    Mutagenesisi149 – 1491T → S: No change in DNA-binding and in response to c-di-GMP. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 230230CRP-like protein ClpPRO_0000405702Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi314565.XC_0486.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UZF6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini158 – 23073HTH crp-typePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Binding of c-di-GMP appears to trigger the active Clp conformation into an open form or inactive state, hence abolishing its DNA-binding ability.By similarity

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0664.
    HOGENOMiHOG000250565.
    KOiK10914.
    OMAiVTINPEN.
    OrthoDBiEOG69GZGV.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view]
    PRINTSiPR00034. HTHCRP.
    SMARTiSM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4UZF6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLGNTTVVT TTVRNATPSL TLDAGTIERF LAHSHRRRYP TRTDVFRPGD    50
    PAGTLYYVIS GSVSIIAEED DDRELVLGYF GSGEFVGEMG LFIESDTREV 100
    ILRTRTQCEL AEISYERLQQ LFQTSLSPDA PRILYAIGVQ LSKRLLDTTR 150
    KASRLAFLDV TDRIVRTLHD LSKEPEAMSH PQGTQLRVSR QELARLVGCS 200
    REMAGRVLKK LQADGLLHAR GKTVVLYGTR 230
    Length:230
    Mass (Da):25,711
    Last modified:July 5, 2005 - v1
    Checksum:iF9252D2A1D2C1F0B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000050 Genomic DNA. Translation: AAY47567.1.
    RefSeqiYP_241587.1. NC_007086.1.

    Genome annotation databases

    EnsemblBacteriaiAAY47567; AAY47567; XC_0486.
    GeneIDi3382910.
    KEGGixcb:XC_0486.
    PATRICi24062214. VBIXanCam24967_0519.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000050 Genomic DNA. Translation: AAY47567.1 .
    RefSeqi YP_241587.1. NC_007086.1.

    3D structure databases

    ProteinModelPortali Q4UZF6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 314565.XC_0486.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY47567 ; AAY47567 ; XC_0486 .
    GeneIDi 3382910.
    KEGGi xcb:XC_0486.
    PATRICi 24062214. VBIXanCam24967_0519.

    Phylogenomic databases

    eggNOGi COG0664.
    HOGENOMi HOG000250565.
    KOi K10914.
    OMAi VTINPEN.
    OrthoDBi EOG69GZGV.

    Enzyme and pathway databases

    BioCyci XCAM314565:GCQG-483-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR012318. HTH_CRP_2.
    IPR014710. RmlC-like_jellyroll.
    IPR001808. Tscrpt_reg_HTH_Crp.
    IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00325. Crp. 1 hit.
    [Graphical view ]
    PRINTSi PR00034. HTHCRP.
    SMARTi SM00100. cNMP. 1 hit.
    SM00419. HTH_CRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    PS00042. HTH_CRP_1. 1 hit.
    PS51063. HTH_CRP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris."
      Qian W., Jia Y., Ren S.-X., He Y.-Q., Feng J.-X., Lu L.-F., Sun Q., Ying G., Tang D.-J., Tang H., Wu W., Hao P., Wang L., Jiang B.-L., Zeng S., Gu W.-Y., Lu G., Rong L.
      , Tian Y., Yao Z., Fu G., Chen B., Fang R., Qiang B., Chen Z., Zhao G.-P., Tang J.-L., He C.
      Genome Res. 15:757-767(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 8004.
    2. "The cyclic nucleotide monophosphate domain of Xanthomonas campestris global regulator Clp defines a new class of cyclic di-GMP effectors."
      Tao F., He Y.W., Wu D.H., Swarup S., Zhang L.H.
      J. Bacteriol. 192:1020-1029(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, ENZYME REGULATION, MUTAGENESIS OF GLU-99 AND THR-149.
      Strain: 8004.

    Entry informationi

    Entry nameiCLP_XANC8
    AccessioniPrimary (citable) accession number: Q4UZF6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 8, 2011
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3