Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q4UZF6

- CLP_XANC8

UniProt

Q4UZF6 - CLP_XANC8

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

CRP-like protein Clp

Gene

clp

Organism
Xanthomonas campestris pv. campestris (strain 8004)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Global transcriptional regulator that regulates virulence factors production by activating or repressing the expression of a large set of genes in diffusible signal factor (DSF) pathway.1 Publication

Enzyme regulationi

Allosterically inhibited by cyclic di-GMP (c-di-GMP), which binds to Clp and abolishes its ability to bind its target gene promoter.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 139122cNMPAdd
BLAST
DNA bindingi190 – 20920H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. catalytic activity Source: UniProtKB-KW
  2. cyclic-di-GMP binding Source: UniProtKB
  3. DNA binding Source: UniProtKB
  4. protein dimerization activity Source: UniProtKB
  5. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
  2. regulation of transcription, DNA-templated Source: UniProtKB
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Virulence

Keywords - Ligandi

c-di-GMP, DNA-binding

Enzyme and pathway databases

BioCyciXCAM314565:GCQG-483-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CRP-like protein Clp
Alternative name(s):
Catabolite activation-like protein
Short name:
CAP-like
Gene namesi
Name:clp
Ordered Locus Names:XC_0486
OrganismiXanthomonas campestris pv. campestris (strain 8004)
Taxonomic identifieri314565 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000000420: Chromosome

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi99 – 991E → S: No change in DNA-binding, but decrease in response to c-di-GMP. 1 Publication
Mutagenesisi149 – 1491T → S: No change in DNA-binding and in response to c-di-GMP. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230CRP-like protein ClpPRO_0000405702Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi314565.XC_0486.

Structurei

3D structure databases

ProteinModelPortaliQ4UZF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini158 – 23073HTH crp-typePROSITE-ProRule annotationAdd
BLAST

Domaini

Binding of c-di-GMP appears to trigger the active Clp conformation into an open form or inactive state, hence abolishing its DNA-binding ability.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000250565.
KOiK10914.
OMAiVTINPEN.
OrthoDBiEOG69GZGV.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UZF6 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLGNTTVVT TTVRNATPSL TLDAGTIERF LAHSHRRRYP TRTDVFRPGD
60 70 80 90 100
PAGTLYYVIS GSVSIIAEED DDRELVLGYF GSGEFVGEMG LFIESDTREV
110 120 130 140 150
ILRTRTQCEL AEISYERLQQ LFQTSLSPDA PRILYAIGVQ LSKRLLDTTR
160 170 180 190 200
KASRLAFLDV TDRIVRTLHD LSKEPEAMSH PQGTQLRVSR QELARLVGCS
210 220 230
REMAGRVLKK LQADGLLHAR GKTVVLYGTR
Length:230
Mass (Da):25,711
Last modified:July 5, 2005 - v1
Checksum:iF9252D2A1D2C1F0B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000050 Genomic DNA. Translation: AAY47567.1.
RefSeqiYP_241587.1. NC_007086.1.

Genome annotation databases

EnsemblBacteriaiAAY47567; AAY47567; XC_0486.
GeneIDi3382910.
KEGGixcb:XC_0486.
PATRICi24062214. VBIXanCam24967_0519.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000050 Genomic DNA. Translation: AAY47567.1 .
RefSeqi YP_241587.1. NC_007086.1.

3D structure databases

ProteinModelPortali Q4UZF6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 314565.XC_0486.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY47567 ; AAY47567 ; XC_0486 .
GeneIDi 3382910.
KEGGi xcb:XC_0486.
PATRICi 24062214. VBIXanCam24967_0519.

Phylogenomic databases

eggNOGi COG0664.
HOGENOMi HOG000250565.
KOi K10914.
OMAi VTINPEN.
OrthoDBi EOG69GZGV.

Enzyme and pathway databases

BioCyci XCAM314565:GCQG-483-MONOMER.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
IPR001808. Tscrpt_reg_HTH_Crp.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF00325. Crp. 1 hit.
[Graphical view ]
PRINTSi PR00034. HTHCRP.
SMARTi SM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris."
    Qian W., Jia Y., Ren S.-X., He Y.-Q., Feng J.-X., Lu L.-F., Sun Q., Ying G., Tang D.-J., Tang H., Wu W., Hao P., Wang L., Jiang B.-L., Zeng S., Gu W.-Y., Lu G., Rong L.
    , Tian Y., Yao Z., Fu G., Chen B., Fang R., Qiang B., Chen Z., Zhao G.-P., Tang J.-L., He C.
    Genome Res. 15:757-767(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 8004.
  2. "The cyclic nucleotide monophosphate domain of Xanthomonas campestris global regulator Clp defines a new class of cyclic di-GMP effectors."
    Tao F., He Y.W., Wu D.H., Swarup S., Zhang L.H.
    J. Bacteriol. 192:1020-1029(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, ENZYME REGULATION, MUTAGENESIS OF GLU-99 AND THR-149.
    Strain: 8004.

Entry informationi

Entry nameiCLP_XANC8
AccessioniPrimary (citable) accession number: Q4UZF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: July 5, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3