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Q4UYT2

- LIPA_XANC8

UniProt

Q4UYT2 - LIPA_XANC8

Protein

Lipoyl synthase

Gene

lipA

Organism
Xanthomonas campestris pv. campestris (strain 8004)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi111 – 1111Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi114 – 1141Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciXCAM314565:GCQG-711-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:XC_0713
    OrganismiXanthomonas campestris pv. campestris (strain 8004)
    Taxonomic identifieri314565 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
    ProteomesiUP000000420: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 337337Lipoyl synthasePRO_1000012298Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi314565.XC_0713.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UYT2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4UYT2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQPIARSIP LQVVSGDTAA PASLQTGVKQ IGGDKINRSP VQFVDAPVLR    50
    KPSWIRVRIP SGNAVQNLKA KLRENRLVTV CEEASCPNIH ECFSHGTATF 100
    MILGEVCTRR CSFCDVAHGR PKPPDASEPA SLATTVADMG LKYVVVTSVD 150
    RDDLRDGGAQ HFVDCISAIR ASAPKTRIEI LTPDFRGKGR MDRALEILAT 200
    SPPDVFNHNI ETVPDLYPNV RPGADYQWSL TLLQRFKAQH PTIATKSGIM 250
    LGLGETMEQV QVTLRDLRAH DVDMITIGQY LQPTPHHHPV MRYWTPEEYK 300
    ALEEYGNALG FSHVASGPMV RSSYHADRQA AGAGVAA 337
    Length:337
    Mass (Da):36,896
    Last modified:July 5, 2005 - v1
    Checksum:iA8B39C9640323148
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000050 Genomic DNA. Translation: AAY47791.1.
    RefSeqiWP_011038549.1. NC_007086.1.
    YP_241811.1. NC_007086.1.

    Genome annotation databases

    EnsemblBacteriaiAAY47791; AAY47791; XC_0713.
    GeneIDi3381494.
    KEGGixcb:XC_0713.
    PATRICi24062718. VBIXanCam24967_0769.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000050 Genomic DNA. Translation: AAY47791.1 .
    RefSeqi WP_011038549.1. NC_007086.1.
    YP_241811.1. NC_007086.1.

    3D structure databases

    ProteinModelPortali Q4UYT2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 314565.XC_0713.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY47791 ; AAY47791 ; XC_0713 .
    GeneIDi 3381494.
    KEGGi xcb:XC_0713.
    PATRICi 24062718. VBIXanCam24967_0769.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci XCAM314565:GCQG-711-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris."
      Qian W., Jia Y., Ren S.-X., He Y.-Q., Feng J.-X., Lu L.-F., Sun Q., Ying G., Tang D.-J., Tang H., Wu W., Hao P., Wang L., Jiang B.-L., Zeng S., Gu W.-Y., Lu G., Rong L.
      , Tian Y., Yao Z., Fu G., Chen B., Fang R., Qiang B., Chen Z., Zhao G.-P., Tang J.-L., He C.
      Genome Res. 15:757-767(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 8004.

    Entry informationi

    Entry nameiLIPA_XANC8
    AccessioniPrimary (citable) accession number: Q4UYT2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3