ID   GFA_XANC8               Reviewed;         191 AA.
AC   Q4UYL9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=Glutathione-dependent formaldehyde-activating enzyme;
DE            EC=4.4.1.22;
DE   AltName: Full=S-(hydroxymethyl)glutathione synthase;
GN   Name=gfa; OrderedLocusNames=XC_0776;
OS   Xanthomonas campestris pv. campestris (strain 8004).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=314565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15899963; DOI=10.1101/gr.3378705;
RA   Qian W., Jia Y., Ren S.-X., He Y.-Q., Feng J.-X., Lu L.-F., Sun Q.,
RA   Ying G., Tang D.-J., Tang H., Wu W., Hao P., Wang L., Jiang B.-L.,
RA   Zeng S., Gu W.-Y., Lu G., Rong L., Tian Y., Yao Z., Fu G., Chen B.,
RA   Fang R., Qiang B., Chen Z., Zhao G.-P., Tang J.-L., He C.;
RT   "Comparative and functional genomic analyses of the pathogenicity of
RT   phytopathogen Xanthomonas campestris pv. campestris.";
RL   Genome Res. 15:757-767(2005).
CC   -!- FUNCTION: Catalyzes the condensation of formaldehyde and
CC       glutathione to S-hydroxymethylglutathione (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-(hydroxymethyl)glutathione = glutathione +
CC       formaldehyde.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate
CC       from formaldehyde (glutathione route): step 1/3.
CC   -!- SIMILARITY: Belongs to the gfa family.
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DR   EMBL; CP000050; AAY47854.1; -; Genomic_DNA.
DR   RefSeq; YP_241874.1; -.
DR   GeneID; 3382417; -.
DR   GenomeReviews; CP000050_GR; XC_0776.
DR   KEGG; xcb:XC_0776; -.
DR   HOGENOM; Q4UYL9; -.
DR   OMA; Q4UYL9; HACGCTK.
DR   BioCyc; XCAM314565:XC_0776-MON; -.
DR   GO; GO:0051907; F:S-(hydroxymethyl)glutathione synthase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:HAMAP.
DR   HAMAP; MF_00723; -; 1.
DR   InterPro; IPR014185; Formald_GSH.
DR   InterPro; IPR006913; GFA.
DR   Pfam; PF04828; GFA; 1.
DR   PIRSF; PIRSF033318; Formald_GSH; 1.
DR   TIGRFAMs; TIGR02820; formald_GSH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Lyase; Metal-binding; Zinc.
FT   CHAIN         1    191       Glutathione-dependent formaldehyde-
FT                                activating enzyme.
FT                                /FTId=PRO_1000045842.
FT   METAL        29     29       Zinc 1; structural (By similarity).
FT   METAL        31     31       Zinc 1; structural (By similarity).
FT   METAL        50     50       Zinc 2; catalytic (By similarity).
FT   METAL        52     52       Zinc 2; catalytic (By similarity).
FT   METAL        55     55       Zinc 2; catalytic (By similarity).
FT   METAL        97     97       Zinc 1; structural (By similarity).
FT   METAL       100    100       Zinc 1; structural (By similarity).
SQ   SEQUENCE   191 AA;  20206 MW;  4FF349EF9358B949 CRC64;
     MANVSIHPAV DGGVVHGSTE GFAGGTLQCL CASNKVTVDV ASQSAHNHAC GCSKCWKPEG
     AKFSVVAVAP RDKVTVTAHP EKLKIVDESA TIQRHACTGC GVHLYGRIEN KDHAFYGLDF
     IHTELSQQSG WSPPGFAAFV SSIIETGTPP DQMDGVRARL TELGLTPYDC LSPALMDALS
     TNVARHKGLL H
//