Reviewed, UniProtKB/Swiss-Prot Q4UYL9 (GFA_XANC8)

Names and origin

Protein names

Recommended name:
    Glutathione-dependent formaldehyde-activating enzyme
    EC=4.4.1.22
Alternative name(s):
    S-(hydroxymethyl)glutathione synthase

Gene names

Name:	gfa
Ordered Locus Names:	XC_0776

Organism

Xanthomonas campestris pv. campestris (strain 8004) [Complete proteome] [HAMAP]

Taxonomic identifier

314565 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione By similarity.
Catalytic activity	S-(hydroxymethyl)glutathione = glutathione + formaldehyde. HAMAP MF_00723
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	One-carbon metabolism; formaldehyde degradation; formate from formaldehyde (glutathione route): step 1/3. HAMAP MF_00723
Sequence similarities	Belongs to the gfa family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	formaldehyde catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	S-(hydroxymethyl)glutathione synthase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	Glutathione-dependent formaldehyde-activating enzyme HAMAP MF_00723		PRO_1000045842
Sites
	Metal binding	29	1	Zinc 1; structural By similarity
	Metal binding	31	1	Zinc 1; structural By similarity
	Metal binding	50	1	Zinc 2; catalytic By similarity
	Metal binding	52	1	Zinc 2; catalytic By similarity
	Metal binding	55	1	Zinc 2; catalytic By similarity
	Metal binding	97	1	Zinc 1; structural By similarity
	Metal binding	100	1	Zinc 1; structural By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UYL9-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 4FF349EF9358B949	FASTA	191	20,206
	10 20 30 40 50 60 MANVSIHPAV DGGVVHGSTE GFAGGTLQCL CASNKVTVDV ASQSAHNHAC GCSKCWKPEG 70 80 90 100 110 120 AKFSVVAVAP RDKVTVTAHP EKLKIVDESA TIQRHACTGC GVHLYGRIEN KDHAFYGLDF 130 140 150 160 170 180 IHTELSQQSG WSPPGFAAFV SSIIETGTPP DQMDGVRARL TELGLTPYDC LSPALMDALS 190 TNVARHKGLL H

References

[1]

"Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris." Qian W., Jia Y., Ren S.-X., He Y.-Q., Feng J.-X., Lu L.-F., Sun Q., Ying G., Tang D.-J., Tang H., Wu W., Hao P., Wang L., Jiang B.-L., Zeng S., Gu W.-Y., Lu G., Rong L. , Tian Y., Yao Z., Fu G., Chen B., Fang R., Qiang B., Chen Z., Zhao G.-P., Tang J.-L., He C. Genome Res. 15:757-767(2005) [PubMed: 15899963] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000050 Genomic DNA. Translation: AAY47854.1.
RefSeq	YP_241874.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3382417.
GenomeReviews	Gene locus XC_0776 in contig CP000050_GR.
KEGG	xcb:XC_0776.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UYL9.
OMA	Q4UYL9. HACGCTK.
Enzyme and pathway databases
BioCyc	XCAM314565:XC_0776-MON.
Family and domain databases
HAMAP	MF_00723. [Tree]
InterPro	IPR014185. Formald_GSH. IPR006913. GFA. [Graphical view]
Pfam	PF04828. GFA. 1 hit. [Graphical view]
PIRSF	PIRSF033318. Formald_GSH. 1 hit.
TIGRFAMs	TIGR02820. formald_GSH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GFA_XANC8

Accession

Primary (citable) accession number: Q4UYL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)