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Protein

Phosphomethylpyrimidine synthase

Gene

thiC

Organism
Xanthomonas campestris pv. campestris (strain 8004)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.UniRule annotation

Catalytic activityi

5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphomethyl)pyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei230SubstrateUniRule annotation1
Binding sitei259SubstrateUniRule annotation1
Binding sitei288SubstrateUniRule annotation1
Binding sitei324SubstrateUniRule annotation1
Binding sitei424SubstrateUniRule annotation1
Metal bindingi428ZincUniRule annotation1
Binding sitei451SubstrateUniRule annotation1
Metal bindingi492ZincUniRule annotation1
Metal bindingi572Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi575Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi580Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphomethylpyrimidine synthaseUniRule annotation (EC:4.1.99.17UniRule annotation)
Alternative name(s):
Hydroxymethylpyrimidine phosphate synthaseUniRule annotation
Short name:
HMP-P synthaseUniRule annotation
Short name:
HMP-phosphate synthaseUniRule annotation
Short name:
HMPP synthaseUniRule annotation
Thiamine biosynthesis protein ThiCUniRule annotation
Gene namesi
Name:thiCUniRule annotation
Ordered Locus Names:XC_0846
OrganismiXanthomonas campestris pv. campestris (strain 8004)
Taxonomic identifieri314565 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000000420 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002423181 – 625Phosphomethylpyrimidine synthaseAdd BLAST625

Proteomic databases

PRIDEiQ4UYF2

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi314565.XC_0846

Structurei

3D structure databases

ProteinModelPortaliQ4UYF2
SMRiQ4UYF2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni344 – 346Substrate bindingUniRule annotation3
Regioni385 – 388Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ThiC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CBF Bacteria
COG0422 LUCA
HOGENOMiHOG000224484
KOiK03147
OMAiTWELFRD
OrthoDBiPOG091H02FB

Family and domain databases

Gene3Di3.20.20.540, 1 hit
HAMAPiMF_00089 ThiC, 1 hit
InterProiView protein in InterPro
IPR037509 ThiC
IPR025747 ThiC-associated_dom
IPR038521 ThiC/Bza_sf
IPR002817 ThiC/BzaA/B
PANTHERiPTHR30557 PTHR30557, 1 hit
PfamiView protein in Pfam
PF13667 ThiC-associated, 1 hit
PF01964 ThiC_Rad_SAM, 1 hit
SFLDiSFLDF00407 phosphomethylpyrimidine_syntha, 1 hit
TIGRFAMsiTIGR00190 thiC, 1 hit

Sequencei

Sequence statusi: Complete.

Q4UYF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAAPTVLLQ QTQTLSAAVT QPIPGSRKIF VQGSRADLQV PMREIVLTRT
60 70 80 90 100
PTLFGGQDNP PLSVYDTSGP YTDPQAAIDL AAGLAPLRAD WIAERGDTLP
110 120 130 140 150
LEALSSSFGR GREHDARLDA VRFPSRRLPR VARSGANVTQ MHYARRGIIT
160 170 180 190 200
PEMEFVAIRE NQRLEAVTDA VLRKQHPGEA FGAAIQQRIT PEFVREEIAR
210 220 230 240 250
GRAILPNNIN HPESEPMIIG RNFLTKINAN IGNSAVSSGI AEEVEKLVWS
260 270 280 290 300
IRWGGDTVMD LSTGKHIHET RDWIIRNSPV PIGTVPIYQA LEKVDGRAEE
310 320 330 340 350
LTWEIFRDTL IEQAEQGVDY FTIHAGVLLR YVPLTARRVT GIVSRGGSIL
360 370 380 390 400
AKWCLAHHKE NFLYTHFEDI CEIMKAYDVA FSLGDGLRPG CIADANDAAQ
410 420 430 440 450
FGELETLGEL TKLAWKHDVQ TMIEGPGHVP MQLIKENMDK QLRECGEAPF
460 470 480 490 500
YTLGPLTTDI APGYDHITSA IGAAMIGWFG TAMLCYVTPK EHLGLPNRQD
510 520 530 540 550
VRDGIMAYKI AAHAADLAKG HPGVQVRDNA LSKARFEFRW DDQFHLGLDP
560 570 580 590 600
EKAKEFHDET LPKDAHKLAH FCSMCGPHFC SMKITQDVRD YAAEHGISED
610 620
HALEAGMQEK SGEFVAQGAQ VYRAS
Length:625
Mass (Da):69,174
Last modified:July 5, 2005 - v1
Checksum:i43EA4014CA3404E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000050 Genomic DNA Translation: AAY47921.1
RefSeqiWP_011038418.1, NC_007086.1

Genome annotation databases

EnsemblBacteriaiAAY47921; AAY47921; XC_0846
GeneIDi35546183
KEGGixcb:XC_0846

Similar proteinsi

Entry informationi

Entry nameiTHIC_XANC8
AccessioniPrimary (citable) accession number: Q4UYF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: July 5, 2005
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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