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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Xanthomonas campestris pv. campestris (strain 8004)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75S-adenosyl-L-methionineUniRule annotation1
Binding sitei100S-adenosyl-L-methionineUniRule annotation1
Binding sitei127S-adenosyl-L-methionineUniRule annotation1
Active sitei150By similarity1
Binding sitei150S-adenosyl-L-methionineUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Binding sitei186SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:XC_1014
OrganismiXanthomonas campestris pv. campestris (strain 8004)
Taxonomic identifieri314565 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000000420 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002292091 – 261tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST261

Interactioni

Protein-protein interaction databases

STRINGi314565.XC_1014.

Structurei

3D structure databases

ProteinModelPortaliQ4UXY5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 161Interaction with RNAUniRule annotation6
Regioni223 – 226Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiIKLGHGV.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UXY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDPFTSDGA KMPPKPFTIE EGRRQVRSFV LRQGRFTPAQ QRAFDELWPR
60 70 80 90 100
FGLDYTGAPR DLDAVFGRPA PKVLEIGFGN GAALRFAAQH DPARDYIGIE
110 120 130 140 150
VHAPGVGRLL NALDDDGATH VRLYHHDAVE VLEREIADGA LDEVRIYFPD
160 170 180 190 200
PWHKKRHNKR RLVQPAFAQL LVRKLRDGGR LHAATDWADY AEQMWDVLDA
210 220 230 240 250
TEGLVNRAGP RGHVARPAWR PQTHFETRGQ KLGHGVWDLL YDRESGMENR
260
ESPGQAPAAP G
Length:261
Mass (Da):29,376
Last modified:July 5, 2005 - v1
Checksum:i6917C437FCFFDFA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000050 Genomic DNA. Translation: AAY48088.1.
RefSeqiWP_011038258.1. NC_007086.1.

Genome annotation databases

EnsemblBacteriaiAAY48088; AAY48088; XC_1014.
KEGGixcb:XC_1014.
PATRICi24063373. VBIXanCam24967_1096.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000050 Genomic DNA. Translation: AAY48088.1.
RefSeqiWP_011038258.1. NC_007086.1.

3D structure databases

ProteinModelPortaliQ4UXY5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi314565.XC_1014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY48088; AAY48088; XC_1014.
KEGGixcb:XC_1014.
PATRICi24063373. VBIXanCam24967_1096.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000073968.
KOiK03439.
OMAiIKLGHGV.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_XANC8
AccessioniPrimary (citable) accession number: Q4UXY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.