Reviewed, UniProtKB/Swiss-Prot Q4UWU6 (CHEB1_XANC8)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	XC_1409

Organism

Xanthomonas campestris pv. campestris (strain 8004) [Complete proteome] [HAMAP]

Taxonomic identifier

314565 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225490
Regions
	Domain	1 – 116	116	Response regulatory
	Domain	162 – 352	191	CheB-type methylesterase
Sites
	Active site	174	1	By similarity
	Active site	200	1	By similarity
	Active site	296	1	By similarity
Amino acid modifications
	Modified residue	50	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UWU6-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 5C2AE933B83FFE84	FASTA	352	37,298
	10 20 30 40 50 60 MVVDDSAVVR QVLVGVLNEA PGIEVIATAA DPLLAIEKMR QQWPDVIVLD VEMPRMDGIT 70 80 90 100 110 120 FLRKIMSERP TPVVICSTLT EKGARVTMDA LAAGAVAVVT KPRLGLKQFL TDSADELVAT 130 140 150 160 170 180 VRSAARANVK RLAARVTAAP LEAEVKHTAD VILPAQTGRA LAQTTERIVA IGTSTGGTQA 190 200 210 220 230 240 LEEVLTALPR VCPGIVIVQH MPEKFTAAFA ARLNGLCQIA VKEAANNDRV MPGRALIAPG 250 260 270 280 290 300 GKHLLLRRSG AQYFVEVLEG PPVNRHRPSV DVLFRSAARA AGSNALGIIM TGMGDDGAAG 310 320 330 340 350 LLEMRQAGAR TIAQDEHTSI VFGMPKEAIK RGGADRILPL GAMAREIVTQ LQ

References

[1]

"Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris." Qian W., Jia Y., Ren S.-X., He Y.-Q., Feng J.-X., Lu L.-F., Sun Q., Ying G., Tang D.-J., Tang H., Wu W., Hao P., Wang L., Jiang B.-L., Zeng S., Gu W.-Y., Lu G., Rong L. , Tian Y., Yao Z., Fu G., Chen B., Fang R., Qiang B., Chen Z., Zhao G.-P., Tang J.-L., He C. Genome Res. 15:757-767(2005) [PubMed: 15899963] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000050 Genomic DNA. Translation: AAY48477.1.
RefSeq	YP_242497.2.
3D structure databases
SMR	Q4UWU6. Positions 1-351.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4UWU6.
Genome annotation databases
GeneID	3382346.
GenomeReviews	Gene locus XC_1409 in contig CP000050_GR.
KEGG	xcb:XC_1409.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	PGGKHML.
PhylomeDB	Q4UWU6.
Enzyme and pathway databases
BioCyc	XCAM314565:XC_1409-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_XANC8

Accession

Primary (citable) accession number: Q4UWU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 5, 2005
Last modified:	January 19, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)