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Q4USS0

- GLND_XANC8

UniProt

Q4USS0 - GLND_XANC8

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Xanthomonas campestris pv. campestris (strain 8004)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciXCAM314565:GCQG-2869-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:XC_2855
    OrganismiXanthomonas campestris pv. campestris (strain 8004)
    Taxonomic identifieri314565 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
    ProteomesiUP000000420: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 869869Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192778Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi314565.XC_2855.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4USS0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini451 – 570120HDUniRule annotationAdd
    BLAST
    Domaini692 – 77180ACT 1UniRule annotationAdd
    BLAST
    Domaini798 – 86972ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 332332UridylyltransferaseAdd
    BLAST
    Regioni333 – 691359Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4USS0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTATPADRPD PGVAGDADWA AEARPLLVHA DMRLCKRFDQ GEPTERLLAL    50
    RARAVDQLMR NAWTRCIPAD AGLSLHAVGG YGRGELFPRS DVDLLVLGET 100
    AAQQRHEQAL ARLFALLWDV GLPISHAVRS PAQCTSAAAD QTVLTALIES 150
    RPLVADAQAR AALAAAIAPQ QVWPPRAFFQ AKREELHARH QRFGDTADNL 200
    EPDIKDGPGG LRDLQTLGWM ALRAFGVKDL EALVGLGHVG MDEAAALRRE 250
    REELARLRYG LHLVANRPEE RLRFDYQKTL AERLGFADDP ESLGVEKMMQ 300
    RFYRSAALIR RISDRLLQRF EEQFDGEAVP VQLDAGFSLR RGYLTADADT 350
    WPDGDVVQVF ALFAQWAAHR EVRGLHSLTA RALAEVLRDL PAYDVADAIA 400
    RDRFMALLRG PRAVETLNRM ARLGVLGQWI PAFASVSGRM QFDLFHVYTV 450
    DQHTLMVLRN IALFAAGRAD ERFSITHEVW PRLRKPELLL LAGLFHDIAK 500
    GRGGDHSELG AVDARAFCLA HRLSEGDTEL VTWLVEQHLR MSVTAQKQDI 550
    SDPEVIHRFA TLVGTRERLD YLYLLTCADI AGTSPKLWNA WKDRLLADLY 600
    FAARRALREG LEHPPPREER LREARESART LMQAQGHDDA TIDRQFAGMP 650
    DENFLRFRPE QLAWQAASLI EVQIGQTLVK ARRAVPDNDA LEVFVYSPDR 700
    DGLFSAIVAT LDRKGYGIHR ARVLDAPHDA IFDVFEVLPQ DSSADGDPQR 750
    LAAALRQVLA GDLLKVRPSR RAVPRQLRHF RFAPRVEFSE SAGGRRTRIS 800
    LVAPDRPGLL ADVAHVLRMQ HLRVHDARIA TFGERAEDQF QITDEHDRPL 850
    PDAARQALRD ALCACLDPT 869
    Length:869
    Mass (Da):97,246
    Last modified:July 5, 2005 - v1
    Checksum:iBC86E2D0BBB9159B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000050 Genomic DNA. Translation: AAY49903.1.
    RefSeqiYP_243923.1. NC_007086.1.

    Genome annotation databases

    EnsemblBacteriaiAAY49903; AAY49903; XC_2855.
    GeneIDi3381388.
    KEGGixcb:XC_2855.
    PATRICi24067289. VBIXanCam24967_3023.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000050 Genomic DNA. Translation: AAY49903.1 .
    RefSeqi YP_243923.1. NC_007086.1.

    3D structure databases

    ProteinModelPortali Q4USS0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 314565.XC_2855.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY49903 ; AAY49903 ; XC_2855 .
    GeneIDi 3381388.
    KEGGi xcb:XC_2855.
    PATRICi 24067289. VBIXanCam24967_3023.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci XCAM314565:GCQG-2869-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris."
      Qian W., Jia Y., Ren S.-X., He Y.-Q., Feng J.-X., Lu L.-F., Sun Q., Ying G., Tang D.-J., Tang H., Wu W., Hao P., Wang L., Jiang B.-L., Zeng S., Gu W.-Y., Lu G., Rong L.
      , Tian Y., Yao Z., Fu G., Chen B., Fang R., Qiang B., Chen Z., Zhao G.-P., Tang J.-L., He C.
      Genome Res. 15:757-767(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 8004.

    Entry informationi

    Entry nameiGLND_XANC8
    AccessioniPrimary (citable) accession number: Q4USS0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3