Q4UNJ4 (DUS_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA-dihydrouridine synthase
EC=1.-.-.-

Gene names

Name:	dus
Ordered Locus Names:	RF_0012

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs By similarity.
Cofactor	FAD By similarity.
Sequence similarities	Belongs to the dus family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro tRNA dihydrouridine synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Probable tRNA-dihydrouridine synthase		PRO_0000280960

Sequences

	Sequence		Length	Mass (Da)
	Q4UNJ4 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 9D6D3BB5056E3A23	FASTA	325	35,294
	10 20 30 40 50 60 MIKIGDIELS SNVILAPMSD ITDLEFRKLV KRFGAGLVVS EMIASRAMVV KSRQSMQKCA 70 80 90 100 110 120 IVHDDPTSAC VQLAGCEPNV IAEAAKMNED MGAKIIDLNF GCPAKKVVGG YAGSALMRDE 130 140 150 160 170 180 QLAAKIFEAT VKAVKIPITV KMRMGWDDNT KNAPDLAKIA ESSGVQMVTV HGRTRCQFYS 190 200 210 220 230 240 GNADWDFIRS VKEAVKIPVI ANGDITNFAK AKEALQKSGA DGIMVGRGAY GKPWLVSQIA 250 260 270 280 290 300 HYLKTGEEKP APSIAEQLDI VTEHYNAILD YYGESVGVPI ARKHIGWYSS GLPSSAEFRG 310 320 AVNLMNDPAE VKEKIAEFYT SVMKE

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY60863.1.
RefSeq	YP_246028.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UNJ4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3400283.
GenomeReviews	Gene locus RF_0012 in contig CP000053_GR.
KEGG	rfe:RF_0012.
NMPDR	fig|315456.3.peg.494.
PATRIC	17890344. VBIRicFel64634_0018.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG557545.
OMA	VTDQPFR.
ProtClustDB	CLSK870702.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0012-MONOMER.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR024036. tRNA-dHydroUridine_Synthase_C. IPR004652. tRNA_dU_NifR3. IPR001269. tRNA_hU_synthase. IPR018517. tRNA_hU_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:1.10.1200.80. G3DSA:1.10.1200.80. 1 hit.
KO	K05540.
PANTHER	PTHR11082. Du_synth. 1 hit.
Pfam	PF01207. Dus. 1 hit. [Graphical view]
PIRSF	PIRSF006621. Dus. 1 hit.
TIGRFAMs	TIGR00737. NifR3_yhdG. 1 hit.
PROSITE	PS01136. UPF0034. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUS_RICFE

Accession

Primary (citable) accession number: Q4UNJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program