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Q4UNF0

- QUEF_RICFE

UniProt

Q4UNF0 - QUEF_RICFE

Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

    Catalytic activityi

    7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei179 – 1791Thioimide intermediateUniRule annotation
    Active sitei186 – 1861Proton donorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi83 – 842NADPHUniRule annotation
    Nucleotide bindingi247 – 2482NADPHUniRule annotation

    GO - Molecular functioni

    1. oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Source: InterPro
    2. preQ1 synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. queuosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Queuosine biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciRFEL315456:GKEK-59-MONOMER.
    UniPathwayiUPA00392.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
    Alternative name(s):
    7-cyano-7-carbaguanine reductaseUniRule annotation
    NADPH-dependent nitrile oxidoreductaseUniRule annotation
    PreQ(0) reductaseUniRule annotation
    Gene namesi
    Name:queFUniRule annotation
    Ordered Locus Names:RF_0057
    OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
    Taxonomic identifieri315456 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000008548: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 273273NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_0000163053Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi315456.RF_0057.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni81 – 833Substrate bindingUniRule annotation
    Regioni218 – 2192Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0780.
    HOGENOMiHOG000273755.
    KOiK06879.
    OMAiIFCDLMH.
    OrthoDBiEOG6D5G0M.

    Family and domain databases

    HAMAPiMF_00817. QueF_type2.
    InterProiIPR029500. QueF.
    IPR029139. QueF_N.
    IPR016428. QueF_type2.
    [Graphical view]
    PfamiPF14489. QueF. 1 hit.
    PF14819. QueF_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
    TIGRFAMsiTIGR03138. QueF. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4UNF0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLSTSLLGK KSTYKDSYDA TLLFKIPRIN NRNELGINSN NLPFYGVDIW    50
    NTYELSCLNK NGKPWVGVGT FYIPTDSENI VESKSFKLYL NSFNNFVVES 100
    VEELERIILQ DLSNVTHAKV TGRIFPINTK IEFSIPSGKN IDDLDIVCNN 150
    YGPPDNSLIE YEDVLVEEEI NSNLLKSNCL VTGQPDWGTI VIKYKGKKLK 200
    HDSFLKYLIS FRNCNEFAEQ CAERIFTDIK NAINPDFLSI YIVYTRRGGI 250
    DICPYRFTNK SYTLPSDKRF IRQ 273
    Length:273
    Mass (Da):31,353
    Last modified:July 5, 2005 - v1
    Checksum:i766DFC40FDED3981
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY60908.1.
    RefSeqiYP_246073.1. NC_007109.1.

    Genome annotation databases

    EnsemblBacteriaiAAY60908; AAY60908; RF_0057.
    GeneIDi3401240.
    KEGGirfe:RF_0057.
    PATRICi17890444. VBIRicFel64634_0067.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY60908.1 .
    RefSeqi YP_246073.1. NC_007109.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 315456.RF_0057.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY60908 ; AAY60908 ; RF_0057 .
    GeneIDi 3401240.
    KEGGi rfe:RF_0057.
    PATRICi 17890444. VBIRicFel64634_0067.

    Phylogenomic databases

    eggNOGi COG0780.
    HOGENOMi HOG000273755.
    KOi K06879.
    OMAi IFCDLMH.
    OrthoDBi EOG6D5G0M.

    Enzyme and pathway databases

    UniPathwayi UPA00392 .
    BioCyci RFEL315456:GKEK-59-MONOMER.

    Family and domain databases

    HAMAPi MF_00817. QueF_type2.
    InterProi IPR029500. QueF.
    IPR029139. QueF_N.
    IPR016428. QueF_type2.
    [Graphical view ]
    Pfami PF14489. QueF. 1 hit.
    PF14819. QueF_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
    TIGRFAMsi TIGR03138. QueF. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
      Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
      PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-1525 / URRWXCal2.

    Entry informationi

    Entry nameiQUEF_RICFE
    AccessioniPrimary (citable) accession number: Q4UNF0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Rickettsia felis (Rickettsia azadi)
      Rickettsia felis (strain URRWXCal2): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3