Reviewed, UniProtKB/Swiss-Prot Q4UNF0 (QUEF_RICFE)

Names and origin

Protein names

Recommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase

Gene names

Name:	queF
Ordered Locus Names:	RF_0057

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity.
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP queuine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_0000163053

Sequences

	Sequence		Length	Mass (Da)
	Q4UNF0-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 766DFC40FDED3981	FASTA	273	31,353
	10 20 30 40 50 60 MPLSTSLLGK KSTYKDSYDA TLLFKIPRIN NRNELGINSN NLPFYGVDIW NTYELSCLNK 70 80 90 100 110 120 NGKPWVGVGT FYIPTDSENI VESKSFKLYL NSFNNFVVES VEELERIILQ DLSNVTHAKV 130 140 150 160 170 180 TGRIFPINTK IEFSIPSGKN IDDLDIVCNN YGPPDNSLIE YEDVLVEEEI NSNLLKSNCL 190 200 210 220 230 240 VTGQPDWGTI VIKYKGKKLK HDSFLKYLIS FRNCNEFAEQ CAERIFTDIK NAINPDFLSI 250 260 270 YIVYTRRGGI DICPYRFTNK SYTLPSDKRF IRQ

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY60908.1.
RefSeq	YP_246073.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401240.
GenomeReviews	Gene locus RF_0057 in contig CP000053_GR.
KEGG	rfe:RF_0057.
NMPDR	fig|315456.3.peg.539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289139.
OMA	INPFRSN.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0057-MON.
BRENDA	1.7.1.13. 297363.
Family and domain databases
HAMAP	MF_00817. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_RICFE

Accession

Primary (citable) accession number: Q4UNF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	July 5, 2005
Last modified:	December 15, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)