Q4UND5 (KDTA_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-D-manno-octulosonic-acid transferase
EC=2.4.-.-

Alternative name(s):
KDO transferase

Gene names

Name:	waaA
Synonyms:	kdtA
Ordered Locus Names:	RF_0072

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA) By similarity.
Pathway	Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 1/4. Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 2/4. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subcellular location	Cell inner membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sugar binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	3-deoxy-D-manno-octulosonic-acid transferase		PRO_0000286456
Regions
	Transmembrane	2 – 22	21	Helical; Signal-anchor; Potential
	Domain	47 – 93	47	RPE1 insert

Sequences

	Sequence		Length	Mass (Da)
	Q4UND5 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: A8737E3670F53F3E	FASTA	464	52,959
	10 20 30 40 50 60 MMLLYYALSF ILLPIYFIII LIRLLIGKED IRRIQERFAI GKHRQVYSLD FLHNEANKER 70 80 90 100 110 120 FKGDTERRTA AYTSVREDSS TGSTSKLPLE ASYARSLIWI HAASVGESMA ALTLISNISK 130 140 150 160 170 180 RYPDIRFLVT SWTNSSAKIL TAKLPKIAVH QFLPIDNIIF TRKFLKNWQP NLGIFIESEL 190 200 210 220 230 240 WPCTINEGAR QCKLLLVNAR ISDKSFKAWL KRKSFFQLIL KNFSKIIVQS ERDLQKFNEL 250 260 270 280 290 300 GISDAINLGN IKFANEKLPV NQEELSKLSS HLDNRQVVVF ASTHPEDEEV ILPIIKNLKE 310 320 330 340 350 360 QFLDCYIILI PRHPERVKSI IDNCKSHNLS ATAKSQNDLP VLSDDIYIVD RFGEMGLFFS 370 380 390 400 410 420 VATISFIGGS FKQGGHNILE AAYFSNCIIF GPDMSKNTDI AKGVLQNEAA IQIKNGEDLL 430 440 450 460 TKLTYLLSPN NSLELKAYRE NALKFVENNQ KVLDEYLQVI TKFL

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY60923.1.
RefSeq	YP_246088.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UND5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3400302.
GenomeReviews	Gene locus RF_0072 in contig CP000053_GR.
KEGG	rfe:RF_0072.
NMPDR	fig|315456.3.peg.554.
PATRIC	17890478. VBIRicFel64634_0083.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG664037.
OMA	HPQRFDE.
ProtClustDB	PRK05749.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0072-MONOMER.
Family and domain databases
InterPro	IPR007507. Glycos_transf_N. IPR005728. Rickett_RPE. [Graphical view]
KO	K02527.
Pfam	PF04413. Glycos_transf_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01045. RPE1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDTA_RICFE

Accession

Primary (citable) accession number: Q4UND5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program