Q4UND3 (AAT_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate aminotransferase
Short name=AspAT
EC=2.6.1.1

Alternative name(s):
Transaminase A

Gene names

Name:	aatA
Ordered Locus Names:	RF_0074

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC L-phenylalanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Aspartate aminotransferase		PRO_0000273125
Sites
	Binding site	39	1	Aspartate; via amide nitrogen By similarity
	Binding site	125	1	Aspartate By similarity
	Binding site	175	1	Aspartate By similarity
	Binding site	375	1	Aspartate By similarity
Amino acid modifications
	Modified residue	239	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UND3 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 2A7CD6AC91F68343	FASTA	409	45,448
	10 20 30 40 50 60 MSIISTRLNS IKPSPTLAVV KKTLELKKAG VDIIALGAGE PDFDTPDNIK EAAIKAIKDG 70 80 90 100 110 120 FTKYTNVEGM PLLKQAIKDK FKRENNIDYE LDEIIVSTGG KQVIYNLFMA SLDKGDKVII 130 140 150 160 170 180 PAPYWVSYPD MVALSTGTPV FVNCGIENNF KLSAEALERS ITDKTKWLII NSPSNPTGAS 190 200 210 220 230 240 YNFEELENIA KVLRKYPHVN VMSDDIYEHI TFDDFKFYTL AQIAPDLKER IFTVNGVSKA 250 260 270 280 290 300 YSMTGWRIGY GVGSKALIKA MTIIQSQSTS NPCSISQMAA IESLNGPQDY IKPNALNFQK 310 320 330 340 350 360 KRDLALSILK RVKYFECYKP EGAFYLFVKC DKIFGHKTKS GKIIANSNDF AEYLLEEAKV 370 380 390 400 AVVPGIAFGL EGYFRISYAT SMEELEEACI RIERACGSIS IKSSLRGDA

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY60925.1.
RefSeq	YP_246090.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UND3.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0074.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY60925; AAY60925; RF_0074.
GeneID	3400304.
KEGG	rfe:RF_0074.
PATRIC	17890482. VBIRicFel64634_0085.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0436.
HOGENOM	HOG000223062.
KO	K00812.
OMA	EGWTHYT.
ProtClustDB	PRK05764.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-77-MONOMER.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAT_RICFE

Accession

Primary (citable) accession number: Q4UND3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program