Q4UNC8 (ALR_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	RF_0079

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity. HAMAP-Rule MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family. Contains 1 RPE1 insert domain.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Alanine racemase HAMAP-Rule MF_01201		PRO_0000272405
Regions
	Domain	286 – 332	47	RPE1 insert
	Region	73 – 132	60	Unknown insert HAMAP-Rule MF_01201
Sites
	Active site	34	1	Proton acceptor; specific for D-alanine By similarity
	Active site	357	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	193	1	Substrate By similarity
	Binding site	405	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	34	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UNC8 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: B1739EA66F516316	FASTA	462	51,764
	10 20 30 40 50 60 MSLCTLEINL SAIKNNYLLL QDICKTSLVG AAVKANGYGL GAVQISKALI EENCRHFFVA 70 80 90 100 110 120 SSEEGVNLRK ALASWHESVF RHCEKNYTVI RRSNPVKNSV SQNFFNYFSG LQQCFAPRND 130 140 150 160 170 180 GSSIHATTPK ALDNDVNILV LNGVFEHDAL ELIEYNLTPV LNNLKQIEIW QKFSNLKNRL 190 200 210 220 230 240 LPCYLHFNTG INRLGLTHNE IEQLINNRDL LKGLDLQYII SHLAVSEEID NPYNLEQLNR 250 260 270 280 290 300 FKTYLQYFPN VKASLANSGG IFLGQDYHFD LARPGAALYG LNPVIDLSNN LSYKEEFEGD 310 320 330 340 350 360 TERRTAAYIN VREDSSTGST YKLPLEGGYS RGLQNPVTLK APIIHLQNLT LDSHIGYNMT 370 380 390 400 410 420 FTTERDSVIA TLPLGYADGF SRNFSNQGEV FINGRSVPIV GRISMDLINI DVTDLPPLDI 430 440 450 460 FLGQEAEIIG NYCTPDKIAS IIGTIGYEVL TSLGSRYKRI YK

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY60930.1.
RefSeq	YP_246095.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UNC8.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0079.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY60930; AAY60930; RF_0079.
GeneID	3400309.
KEGG	rfe:RF_0079.
PATRIC	17890494. VBIRicFel64634_0091.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HOG000031445.
KO	K01775.
OMA	YCTPDKI.
ProtClustDB	PRK00053.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-82-MONOMER.
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 2 hits.
HAMAP	MF_01201. Ala_racemase. Divergent sequence.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. IPR005728. Rickett_RPE. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR01045. RPE1. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_RICFE

Accession

Primary (citable) accession number: Q4UNC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program