Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q4UNA8 (TRMD_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:RF_0099
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length234 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605

Subunit structure

Homodimer By similarity. HAMAP MF_00605

Subcellular location

Cytoplasm Potential HAMAP MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine-N1-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 234234tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
PRO_0000060444

Regions

Region135 – 1406S-adenosyl-L-methionine binding By similarity

Sites

Binding site1151S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UNA8 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 03BC9F2B3DD24773

FASTA23426,461
        10         20         30         40         50         60 
MFILHATILT VFPEMFPGTL GYSLAGQALH KNIWSYDVIN IRDFGLTKHK NVDDEAYGGG 

        70         80         90        100        110        120 
DGLIMRPDVL GSSIDHALSL NPNAEIYYPS PRGRVFTQSF AKEMLKNKNL IFLCGRYEGI 

       130        140        150        160        170        180 
DERVIEEYNV KEISVGDYIL SGGEIPTLTI LDCLIRLLPG VLMNQNTLSS ESFEEVGEFK 

       190        200        210        220        230 
GGLECGLYTR PEIWRDRAVP SVLLSGNHRL INEWKKEQSR IITKLRRPEL LKDL

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000053 Genomic DNA. Translation: AAY60950.1.
RefSeqYP_246115.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UNA8.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3401068.
GenomeReviewsGene locus RF_0099 in contig CP000053_GR.
KEGGrfe:RF_0099.
NMPDRfig|315456.3.peg.618.
PATRIC17890538. VBIRicFel64634_0112.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG285805.
OMAVCGRFEG.
ProtClustDBPRK00026.

Enzyme and pathway databases

BioCycRFEL315456:RF_0099-MONOMER.

Family and domain databases

HAMAPMF_00605. TrmD.
[Tree]
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
Gene3DG3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KOK00554.
PANTHERPTHR10056. PTHR10056. 1 hit.
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMsTIGR00088. TrmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_RICFE
AccessionPrimary (citable) accession number: Q4UNA8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: July 5, 2005
Last modified: January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents