Q4UN94 (KDSA_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	RF_0113

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000277912

Sequences

	Sequence		Length	Mass (Da)
	Q4UN94 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 103EA00679A60975	FASTA	274	29,971
	10 20 30 40 50 60 MQKVVKLNNI KIGNDLPFVL IAGPCQIEGK DHALFMAEKL VKLTSKLEIP FIYKSSFDKA 70 80 90 100 110 120 NRTSVNGIRG LGIEKGLEIL SKVKSEFDCP IVTDVHSESQ CTETAEIADI LQIPAFLCRQ 130 140 150 160 170 180 TDLLQAAAKT GKIVKVKKGQ FLAPWDMKNV QTKLEAFGVK DILFTERGAC FGYNNLVSDM 190 200 210 220 230 240 RSLAIIAELN VPVVFDATHS VQQPGGLGGS TGGERKYVEL LAKAATAVGI AGMYMEVHQD 250 260 270 PDNAPSDGPC MIKLDNLESI LIKLKKYDKI TKEK

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY60964.1.
RefSeq	YP_246129.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 1LRO based on UniProtKB O66496.
ProteinModelPortal	Q4UN94.
SMR	Q4UN94. Positions 17-271.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3401082.
GenomeReviews	Gene locus RF_0113 in contig CP000053_GR.
KEGG	rfe:RF_0113.
NMPDR	fig|315456.3.peg.632.
PATRIC	17890576. VBIRicFel64634_0131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG575990.
OMA	ITERGAM.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0113-MONOMER.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_RICFE

Accession

Primary (citable) accession number: Q4UN94

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program