Q4UN88 (MNMG_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Alternative name(s):
Glucose-inhibited division protein A

Gene names

Name:	mnmG
Synonyms:	gidA
Ordered Locus Names:	RF_0119

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP MF_00129
Cofactor	FAD By similarity. HAMAP MF_00129
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP MF_00129
Subcellular location	Cytoplasm By similarity HAMAP MF_00129.
Sequence similarities	Belongs to the MnmG family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG HAMAP MF_00129		PRO_0000117165
Regions
	Nucleotide binding	10 – 15	6	FAD By similarity
	Nucleotide binding	269 – 283	15	NAD Potential
Sites
	Binding site	122	1	FAD; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	177	1	FAD By similarity
	Binding site	366	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UN88 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 81BB60FC6B96EB13	FASTA	622	68,619
	10 20 30 40 50 60 MLKYDVIVIG GGHAGVEAAA ASARLGASTL LITLKPENLG EMSCNPAIGG IAKGTLVKEI 70 80 90 100 110 120 DALDGLMGYV IDQGGIHYKM LNETRGPAVW GPRAQADRKL YKKAMCQILT NYPNLDILYG 130 140 150 160 170 180 KVEDIEIKSS KVEAVILNND SKILCQKIIL TTGTFLSGLI HIGQKKIPAG RVDEEPSYGL 190 200 210 220 230 240 SNTLKKVGFK IARLKTGTPP RIDGRTIDYS KTALQPGDKT PRPFSELTSV VNVPQINCFI 250 260 270 280 290 300 TKTTSETHDI IRENLNKSAM YSGQIEGIGP RYCPSIEDKI VRFSTKSEHR IFLEPEGLDD 310 320 330 340 350 360 YTIYPNGIST SLPEDVQHKL IKTIPGLENV KVLRPGYAIE YDYVDPREIS VTLETKKIAG 370 380 390 400 410 420 LYFAGQINGT TGYEEAAGQG IIAGINAALA VKDQAPFMLT RANSYIGVMI DDLTTFGTIE 430 440 450 460 470 480 PYRMFTSRSE YRLSLRADNA DLRLTELGIN IGVVSEKRKK IFTKKCEDIE KTKLLLNTLS 490 500 510 520 530 540 LTTSKLAKMG IQVAQDGTYK TVLDLFKIPS FNVEQAIKIF PMLKEMQNNN ILQLLYIEAK 550 560 570 580 590 600 YASYLTRQHA DINLFQSEEA QLIPKNIDYF KIPSISLEIQ EKLSSHKPTT IGVARRISGI 610 620 TPAAITAIII YLKTKYSDGS ST

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY60970.1.
RefSeq	YP_246135.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UN88.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3401088.
GenomeReviews	Gene locus RF_0119 in contig CP000053_GR.
KEGG	rfe:RF_0119.
NMPDR	fig|315456.3.peg.638.
PATRIC	17890588. VBIRicFel64634_0137.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284774.
OMA	GFDRSPM.
ProtClustDB	PRK05192.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0119-MONOMER.
Family and domain databases
HAMAP	MF_00129. MnmG_GidA. [Tree]
InterPro	IPR004416. GidA. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
KO	K03495.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00136. GidA. 1 hit.
PROSITE	PS01280. GIDA_1. 1 hit. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MNMG_RICFE

Accession

Primary (citable) accession number: Q4UN88

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program