Q4UN68 (FTSH_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent zinc metalloprotease FtsH
EC=3.4.24.-

Gene names

Name:	ftsH
Ordered Locus Names:	RF_0139

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins By similarity. HAMAP MF_01458
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01458
Subunit structure	Homohexamer Potential.
Subcellular location	Cell inner membrane; Multi-pass membrane protein; Cytoplasmic side By similarity HAMAP MF_01458.
Sequence similarities	In the central section; belongs to the AAA ATPase family. In the C-terminal section; belongs to the peptidase M41 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein catabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 635	635	ATP-dependent zinc metalloprotease FtsH HAMAP MF_01458		PRO_0000272336
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Potential
	Topological domain	28 – 103	76	Periplasmic Potential
	Transmembrane	104 – 124	21	Helical; Potential
	Topological domain	125 – 635	511	Cytoplasmic Potential
	Nucleotide binding	195 – 202	8	ATP Potential
Sites
	Active site	418	1	By similarity
	Metal binding	417	1	Zinc; catalytic By similarity
	Metal binding	421	1	Zinc; catalytic By similarity
	Metal binding	495	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UN68 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 9F9E1BFC19AF9514	FASTA	635	69,604
	10 20 30 40 50 60 MNNQGRSILT WAALFVFVIL LFNVFQSDGL LGGRNNITFS DFLTRVDEKT VNSVKIQGRV 70 80 90 100 110 120 IEGTSNDGST FNTYAPDYPD LVNRLTSNDV NIEVVPLETR MNTFLGFLIS WFPMLLLIGV 130 140 150 160 170 180 WVFFMRQMHG GGKAMGFGKS KARLLSDKGP KITFKDVAGI DEAKEELTEI VDFLRDPSKF 190 200 210 220 230 240 QKLGGKIPKG CLLIGPPGTG KTLLAKAIAG EANVPFFSIS GSDFVEMFVG VGASRVRDMF 250 260 270 280 290 300 EQGKRNAPCI IFIDEIDAVG RHRGIGMGGG NDEREQTLNQ MLVEMDGFEA NEGVVIIAAT 310 320 330 340 350 360 NRPDVLDRAL LRPGRFDRQI AVANPDINGR EQILKVHLKK IKYNSTVLAR IIARGTPGFS 370 380 390 400 410 420 GAELANLVNE AALIAARLGK KEVDMHDMEE AKDKVLMGVA RRSIAMSEKE KRLTAYHEGG 430 440 450 460 470 480 HALVGLYCPA ASPLHKATII PRGNALGMVQ RLPETDEYSQ NREQMESSIA VYMAGRVAEE 490 500 510 520 530 540 IIFGRNKVTS GASSDIKGAT NIARAMVTKA GLSDLIGPIF HGSSSDDMYG RQPSNETSEA 550 560 570 580 590 600 TAELIDAEVK KIITQGYEFA KDILTKHIDQ LHTLANALIE YETLSGQQIK NLLSGRALDS 610 620 630 EEENKFPFND SPTIKIDKEK SPEKAKKAKK ESTNI

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY60990.1.
RefSeq	YP_246155.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 2CE7 based on UniProtKB Q9WZ49.
ProteinModelPortal	Q4UN68.
SMR	Q4UN68. Positions 144-399.
ModBase	Search...
Protein family/group databases
MEROPS	M41.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3400640.
GenomeReviews	Gene locus RF_0139 in contig CP000053_GR.
KEGG	rfe:RF_0139.
NMPDR	fig|315456.3.peg.658.
PATRIC	17890632. VBIRicFel64634_0159.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724153.
OMA	KKESTNI.
ProtClustDB	CLSK870717.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0139-MONOMER.
Family and domain databases
HAMAP	MF_01458. FtsH. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR005936. Pept_M41_FtsH. IPR011546. Pept_M41_FtsH_extracell. IPR000642. Peptidase_M41. [Graphical view]
KO	K03798.
Pfam	PF00004. AAA. 1 hit. PF06480. FtsH_ext. 1 hit. PF01434. Peptidase_M41. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01241. FtsH_fam. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTSH_RICFE

Accession

Primary (citable) accession number: Q4UN68

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program