Q4UN67 (TILS_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA(Ile)-lysidine synthase
EC=6.3.4.19

Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase

Gene names

Name:	tilS
Ordered Locus Names:	RF_0140

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity. HAMAP MF_01161
Catalytic activity	(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O. HAMAP MF_01161
Subcellular location	Cytoplasm Probable HAMAP MF_01161.
Domain	The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding. HAMAP MF_01161
Sequence similarities	Belongs to the tRNA(Ile)-lysidine synthase family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity, forming carbon-nitrogen bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	tRNA(Ile)-lysidine synthase HAMAP MF_01161		PRO_0000273103
Regions
	Nucleotide binding	27 – 32	6	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4UN67 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 6FF78670B36EDD70	FASTA	430	50,326
	10 20 30 40 50 60 MLYEKFEYNI NNLIGNFGLS KIAAAVSGGS DSVALLYLAN IWAEKNNIEL SVISVDHNLR 70 80 90 100 110 120 EQSKQETHYI QNISNSLNRK HYSLSFDHQN NFSNLQERAR EGRYDLMTNL CLELDILVLL 130 140 150 160 170 180 TAHHEDDYVE NFCLRLERNS GIFGLSSSNI NWYNNIQIIR PLYNIPKSEL VEYLVSHNIK 190 200 210 220 230 240 WFEDESNSSD KYRRNIIRQK LAKGEDYIKA DIILQQLKIN DLLDNKFKPE LISAIAEAVK 250 260 270 280 290 300 IFEYGFAFLD LVKFDKFSNE VKVQIINFLL IIISGQFRAA RFYSVEPILK LITQDVNFKN 310 320 330 340 350 360 TLHGCVINRI QNELLIYREF GKKLPESKIL LDKSVIWDNR FRITRNQETP NCFVTHLSLE 370 380 390 400 410 420 DYKMIKKQLD LEPLKNLSCK NHNAIIFTLP IIKILEKVIA IPHISYYDND MWNFEVSFAP 430 NFVSRFTHFC

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY60991.1.
RefSeq	YP_246156.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UN67.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3400641.
GenomeReviews	Gene locus RF_0140 in contig CP000053_GR.
KEGG	rfe:RF_0140.
NMPDR	fig|315456.3.peg.659.
PATRIC	17890634. VBIRicFel64634_0160.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG586536.
OMA	NIQIIRP.
ProtClustDB	CLSK2503565.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0140-MONOMER.
Family and domain databases
HAMAP	MF_01161. tRNA(Ile)-lys_synt. [Tree]
InterPro	IPR012094. Lysidine-tRNA-synth. IPR012795. Lysidine-tRNA-synth_N. IPR014729. Rossmann-like_a/b/a_fold. IPR011063. tRNA-lysidine/thiocyt_synth. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K04075.
PANTHER	PTHR11807:SF2. ATPase_MesJ_YaeO. 1 hit.
Pfam	PF01171. ATP_bind_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR02432. Lysidine_TilS_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TILS_RICFE

Accession

Primary (citable) accession number: Q4UN67

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program