Reviewed, UniProtKB/Swiss-Prot Q4UN31 (DPO3E_RICFE)

Names and origin

Protein names

Recommended name:
    DNA polymerase III subunit epsilon
    EC=2.7.7.7

Gene names

Name:	dnaQ
Ordered Locus Names:	RF_0176

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Cofactor	Binds 2 divalent metal cations. Magnesium or manganese By similarity.
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Magnesium Manganese Metal-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	DNA polymerase III subunit epsilon		PRO_0000280953
Sites
	Active site	156	1	Proton acceptor By similarity
	Metal binding	10	1	Divalent metal cation 1; catalytic By similarity
	Metal binding	10	1	Divalent metal cation 2; catalytic By similarity
	Metal binding	12	1	Divalent metal cation 1; catalytic By similarity
	Metal binding	161	1	Divalent metal cation 1; catalytic By similarity
	Binding site	10	1	Substrate By similarity
	Binding site	12	1	Substrate By similarity
	Binding site	55	1	Substrate By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	161	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UN31-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 6E9A94748CE2C0AE	FASTA	229	25,944
	10 20 30 40 50 60 MSSLREIILD TETTGLDPRQ GHRIVEIGAI EMVNKVLTGR NFHFYINPER DMPFDAYRIH 70 80 90 100 110 120 GISGEFLKDK PLFHTIADDF LEFISDSKLI IHNAPFDIKF LNHELSLLKR AEIKLLELSN 130 140 150 160 170 180 AIDTLVMARS MFPGSKYNLD ALCKRFKVDN SGRQLHGALK DAALLAEVYV ELTGGRQSAF 190 200 210 220 KMVDKSVETN NLATNQVNNK TEQATIVIKP TKEELQKHKE FLSSILKTA

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61027.1.
RefSeq	YP_246192.1.
3D structure databases
HSSP	HSSP built from PDB template 1J54 based on UniProtKB P03007.
ModBase	Search...
Genome annotation databases
GeneID	3400593.
GenomeReviews	Gene locus RF_0176 in contig CP000053_GR.
KEGG	rfe:RF_0176.
NMPDR	fig|315456.3.peg.10.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UN31.
OMA	HGITNEF
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0176-MON.
BRENDA	2.7.7.7. 297363.
Family and domain databases
InterPro	IPR006054. DnaQ. IPR006309. DnaQ_proteo. IPR006055. Exonuclease. IPR013520. Exonuclease_RNaseT/DNA_pol3. IPR012337. PolynucTfrase_RNaseH_fold. [Graphical view]
Pfam	PF00929. Exonuc_X-T. 1 hit. [Graphical view]
SMART	SM00479. EXOIII. 1 hit. [Graphical view]
TIGRFAMs	TIGR00573. dnaq. 1 hit. TIGR01406. dnaQ_proteo. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3E_RICFE

Accession

Primary (citable) accession number: Q4UN31

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	July 5, 2005
Last modified:	November 24, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)