Reviewed, UniProtKB/Swiss-Prot Q4UN27 (RNH_RICFE)

Names and origin

Protein names

Recommended name:
    Ribonuclease H
      Short name=RNase H
    EC=3.1.26.4

Gene names

Name:	rnhA
Ordered Locus Names:	RF_0180

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00042
Cofactor	Binds 1 magnesium ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNase H family. Contains 1 RNase H domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 152	152	Ribonuclease H HAMAP MF_00042		PRO_0000195397
Regions
	Domain	1 – 142	142	RNase H
Sites
	Metal binding	10	1	Magnesium 1 By similarity
	Metal binding	10	1	Magnesium 2 By similarity
	Metal binding	48	1	Magnesium 1 By similarity
	Metal binding	70	1	Magnesium 1 By similarity
	Metal binding	134	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UN27-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 96A94F38866FC1F6	FASTA	152	17,057
	10 20 30 40 50 60 MDSKVVIYTD GACAGNPGPG GWGALLQFND TSKEIFGYEL DTTNNRMEIT AALEALRILK 70 80 90 100 110 120 KSCNVEIYTD SKYLQQGITA WIHNWIKNNW CKSNNEPVKN ADLWQKLYAE LSKHTIIWKW 130 140 150 VKGHANNSGN IAADKLAVQG RETAIEILKC RG

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61031.1.
RefSeq	YP_246196.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401672.
GenomeReviews	Gene locus RF_0180 in contig CP000053_GR.
KEGG	rfe:RF_0180.
NMPDR	fig|315456.3.peg.14.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UN27.
OMA	SKVVIYT
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0180-MON.
BRENDA	3.1.26.4. 297363.
Family and domain databases
HAMAP	MF_00042. [Tree]
InterPro	IPR012337. PolynucTfrase_RNaseH_fold. IPR002156. RNase_H. [Graphical view]
Pfam	PF00075. RnaseH. 1 hit. [Graphical view]
PROSITE	PS50879. RNASE_H. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNH_RICFE

Accession

Primary (citable) accession number: Q4UN27

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	July 5, 2005
Last modified:	November 24, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)