Reviewed, UniProtKB/Swiss-Prot Q4UN16 (TGT_RICFE)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	RF_0191

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_0000278058
Sites
	Active site	92	1	Nucleophile By similarity
	Metal binding	304	1	Zinc By similarity
	Metal binding	306	1	Zinc By similarity
	Metal binding	309	1	Zinc By similarity
	Metal binding	335	1	Zinc By similarity
	Binding site	93	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UN16-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 8FCE42EE5D3482CF	FASTA	361	40,592
	10 20 30 40 50 60 MSKFSFNIHH QHKKARNGVI TTAHGEIRTP AFMPVGTRGT VKAMLPESVA ETGADILLGN 70 80 90 100 110 120 TYHLMLQPTA ERIARLGGLH KFMNWDKPIL TDSGGFQVMS LSKLRKITEE GVSFSSHING 130 140 150 160 170 180 DKYMLTPERS TEIQHLLGST ITMAFDECTP YPATFEEAKT SMQLTTRWAN RSRNAFVKRD 190 200 210 220 230 240 GYAQFGIIQG SVYEELREQS ARDLVELDFE GYAIGGLAVG EGQELMFKVL DYAPDFLPQN 250 260 270 280 290 300 KPCYLMGVGK PADIIGAVRR GIDMFDCVIP TRSGRNGQAF TKYGTVNIRN SKYADDNEPL 310 320 330 340 350 360 EHDCLCPACK NYSKAYLHLL VRIGEILGAM LMTWHNLTYF QNLMSRIRKY IKLGKDFDFD S

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61042.1.
RefSeq	YP_246207.1.
3D structure databases
HSSP	HSSP built from PDB template 1Y5X based on UniProtKB P28720.
ModBase	Search...
Genome annotation databases
GeneID	3400380.
GenomeReviews	Gene locus RF_0191 in contig CP000053_GR.
KEGG	rfe:RF_0191.
NMPDR	fig|315456.3.peg.25.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG629929.
OMA	MSLRWAQ.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0191-MON.
BRENDA	2.4.2.29. 297363.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. Queuine_tRNA-ribosylTrfase. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TGT_RICFE

Accession

Primary (citable) accession number: Q4UN16

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 5, 2005
Last modified:	December 15, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)