Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q4UN15 (DNLJ_RICFE)

Last modified November 24, 2009. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    DNA ligase
    EC=6.5.1.2
Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name: ligA
Synonyms: lig
Ordered Locus Names: RF_0192
OrganismRickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier42862 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Hide | Top

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity.

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity.

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Hide | Top

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA ligase (NAD+) activity

Inferred from electronic annotation. Source: HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689DNA ligase HAMAP MF_01588
PRO_0000280947

Regions

Domain610 – 68980BRCT
Nucleotide binding40 – 445NAD By similarity
Nucleotide binding89 – 902NAD By similarity

Sites

Active site1231N6-AMP-lysine intermediate By similarity
Metal binding4131Zinc By similarity
Metal binding4161Zinc By similarity
Metal binding4311Zinc By similarity
Metal binding4371Zinc By similarity
Binding site1211NAD By similarity
Binding site1441NAD By similarity
Binding site1791NAD By similarity
Binding site2951NAD By similarity
Binding site3191NAD By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q4UN15-1 [UniParc].

Last modified March 20, 2007. Version 2.
Checksum: 13C18329860F833A

FASTA68977,634
        10         20         30         40         50         60 
MQNIDLISEE EAKKLLEELA DKIAAYNHAY YIEDNPLVSD SEYDQLFNTN LKLEQKFPHL 

        70         80         90        100        110        120 
VLENSPSKKI GAKITNKFAK ITHQIPMLSL SNAFDEQDVR DFVDRIKNFL RLDEFAPIFC 

       130        140        150        160        170        180 
EPKIDGLSFS AIYKNGLLTT GATRGDGYVG EDITANIKTI KNFPHKIDNA PEFLEVRGEI 

       190        200        210        220        230        240 
YIEKQDFLNL NKEQEEQGRD KFANPRNAAA GSLRQLDASI TAQRSLKYFV YSGGVTEQNL 

       250        260        270        280        290        300 
ASSQEQLLIK LKEFGFSVNE ISKLTNSEEE IFTFYEYLKT NRENLPYEID GVVYKLNDFA 

       310        320        330        340        350        360 
LQNRMGFIAR SPRFATAHKF PAIIGRTKLL SITVQVGRTG TLTPVAELEP IEIGGVTVSR 

       370        380        390        400        410        420 
ATLHNFQEIM RKDVRIDDYV FLQRAGDVIP KITGVDIGKR PNDTIAFDTP LFCPSCNSKL 

       430        440        450        460        470        480 
HYAPEDIIIR CDNGLNCPAQ NYERIRHFVS KNAMDIEGLG RKQVEFLIDK GLISNPLDIF 

       490        500        510        520        530        540 
FLKEKNDSSL TKLENMDGWG KKSVENLFKN IEQSKNVSLP RFIYALGIRH IGEQNAKLLA 

       550        560        570        580        590        600 
REFGSYANFI AQMELLSKND LDIYQKLNDL EGIGDKILVD IIDFFDVKEN TTLIKKLGEV 

       610        620        630        640        650        660 
LNIEDYKETR EQSSLTGKII VFTGSLPTIS RAEAKATAEK LGAKVAASVS SNTDLVVAGV 

       670        680 
DAGSKLKKAK ELNIKIIGEE EWLTLIKNV

« Hide

Hide | Top

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Hide | Top

Cross-references

Sequence databases

CP000053 Genomic DNA. Translation: AAY61043.1. Different initiation.
RefSeqYP_246208.1.

3D structure databases

HSSPHSSP built from PDB template 1B04 based on UniProtKB O87703.
ModBaseSearch...

Genome annotation databases

GeneID3400381.
GenomeReviewsGene locus RF_0192 in contig CP000053_GR.
KEGGrfe:RF_0192.
NMPDRfig|315456.3.peg.26.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ4UN15.
OMAIKHFASR

Enzyme and pathway databases

BioCycRFEL315456:RF_0192-MON.
BRENDA6.5.1.2. 297363.

Family and domain databases

HAMAPMF_01588.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00532. LIGANc. 1 hit.
[Graphical view]
TIGRFAMsTIGR00575. dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameDNLJ_RICFE
AccessionPrimary (citable) accession number: Q4UN15
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: November 24, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents