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Q4UN15 (DNLJ_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA ligase
EC=6.5.1.2
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD+]
Gene names
Name:ligA
Synonyms:lig
Ordered Locus Names:RF_0192
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length689 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA By similarity. HAMAP MF_01588

Catalytic activity

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m). HAMAP MF_01588

Cofactor

Magnesium or manganese By similarity. HAMAP MF_01588

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Contains 1 BRCT domain.

Sequence caution

The sequence AAY61043.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
DNA replication
   LigandMagnesium
Manganese
Metal-binding
NAD
Zinc
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

DNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintracellular

Inferred from electronic annotation. Source: InterPro

   Molecular functionDNA ligase (NAD+) activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 689689DNA ligase HAMAP MF_01588
PRO_0000280947

Regions

Domain610 – 68980BRCT
Nucleotide binding40 – 445NAD By similarity
Nucleotide binding89 – 902NAD By similarity

Sites

Active site1231N6-AMP-lysine intermediate By similarity
Metal binding4131Zinc By similarity
Metal binding4161Zinc By similarity
Metal binding4311Zinc By similarity
Metal binding4371Zinc By similarity
Binding site1211NAD By similarity
Binding site1441NAD By similarity
Binding site1791NAD By similarity
Binding site2951NAD By similarity
Binding site3191NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UN15 [UniParc].

Last modified March 20, 2007. Version 2.
Checksum: 13C18329860F833A

FASTA68977,634
        10         20         30         40         50         60 
MQNIDLISEE EAKKLLEELA DKIAAYNHAY YIEDNPLVSD SEYDQLFNTN LKLEQKFPHL 

        70         80         90        100        110        120 
VLENSPSKKI GAKITNKFAK ITHQIPMLSL SNAFDEQDVR DFVDRIKNFL RLDEFAPIFC 

       130        140        150        160        170        180 
EPKIDGLSFS AIYKNGLLTT GATRGDGYVG EDITANIKTI KNFPHKIDNA PEFLEVRGEI 

       190        200        210        220        230        240 
YIEKQDFLNL NKEQEEQGRD KFANPRNAAA GSLRQLDASI TAQRSLKYFV YSGGVTEQNL 

       250        260        270        280        290        300 
ASSQEQLLIK LKEFGFSVNE ISKLTNSEEE IFTFYEYLKT NRENLPYEID GVVYKLNDFA 

       310        320        330        340        350        360 
LQNRMGFIAR SPRFATAHKF PAIIGRTKLL SITVQVGRTG TLTPVAELEP IEIGGVTVSR 

       370        380        390        400        410        420 
ATLHNFQEIM RKDVRIDDYV FLQRAGDVIP KITGVDIGKR PNDTIAFDTP LFCPSCNSKL 

       430        440        450        460        470        480 
HYAPEDIIIR CDNGLNCPAQ NYERIRHFVS KNAMDIEGLG RKQVEFLIDK GLISNPLDIF 

       490        500        510        520        530        540 
FLKEKNDSSL TKLENMDGWG KKSVENLFKN IEQSKNVSLP RFIYALGIRH IGEQNAKLLA 

       550        560        570        580        590        600 
REFGSYANFI AQMELLSKND LDIYQKLNDL EGIGDKILVD IIDFFDVKEN TTLIKKLGEV 

       610        620        630        640        650        660 
LNIEDYKETR EQSSLTGKII VFTGSLPTIS RAEAKATAEK LGAKVAASVS SNTDLVVAGV 

       670        680 
DAGSKLKKAK ELNIKIIGEE EWLTLIKNV

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References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000053 Genomic DNA. Translation: AAY61043.1. Different initiation.
RefSeqYP_246208.1. NC_007109.1.

3D structure databases

HSSPHSSP built from PDB template 1B04 based on UniProtKB O87703.
ProteinModelPortalQ4UN15.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3400381.
GenomeReviewsGene locus RF_0192 in contig CP000053_GR.
KEGGrfe:RF_0192.
NMPDRfig|315456.3.peg.26.
PATRIC17890754. VBIRicFel64634_0219.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG620317.
OMAENVRTIR.
ProtClustDBPRK07956.

Enzyme and pathway databases

BioCycRFEL315456:RF_0192-MONOMER.

Family and domain databases

HAMAPMF_01588. DNA_ligase_A.
[Tree]
InterProIPR001357. BRCT.
IPR018239. DNA_ligase_AS.
IPR004150. DNA_ligase_OB.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01972.
PfamPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFPIRSF001604. LigA. 1 hit.
SMARTSM00292. BRCT. 1 hit.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMSSF52113. BRCT. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF47781. RuvA_2_like. 1 hit.
TIGRFAMsTIGR00575. Dnlj. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS01055. DNA_LIGASE_N1. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLJ_RICFE
AccessionPrimary (citable) accession number: Q4UN15
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: January 25, 2012
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

SIMILARITY comments

Index of protein domains and families

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