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Q4UMX2 (SYM_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase

EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:RF_0235
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Methionine--tRNA ligase HAMAP-Rule MF_01228
PRO_0000272402

Regions

Motif12 – 2211"HIGH" region HAMAP-Rule MF_01228
Motif295 – 2995"KMSKS" region HAMAP-Rule MF_01228

Sites

Binding site2981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UMX2 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 1F892EDF5ACAA1D8

FASTA51258,800
        10         20         30         40         50         60 
MKNTYYITTP IYYVNDVPHI GHAYTSVASD VIARFIRLCG FDVMFLTGTD EHGQKVEKAA 

        70         80         90        100        110        120 
INKNIDPQKF TDHTSESFRH LMNVMNISND DFIRTTESRH KEAVAIFWQK LLDNGTIYEG 

       130        140        150        160        170        180 
FYEGWYAVRD EAFFDESELT SDGLAPTGAP VEWVKEPSYF FNLSKWQDKL LEFYEANPDF 

       190        200        210        220        230        240 
IRPISRRNEV ISFVKSGLKD LSVSRTTFNW GIKVPNNEKH VIYVWLDALA SYISALGYPD 

       250        260        270        280        290        300 
KQSNYGKFWP ADLHVVGKDI LRFHAVYWPA FLMAAEIPLP KTIMAHGWWT NEGQKISKSL 

       310        320        330        340        350        360 
GNTIDPIKLI DEFGVDQVRY FLMREVTFGA DGNFARSNLV TRINSELSNK IGNLLQRTTA 

       370        380        390        400        410        420 
FVYKNNDGKV PLLMQGIIDK IYELPILKTA SKFAEQNILL MEKTEINKVL ENIINLSEEA 

       430        440        450        460        470        480 
NIYIDSEAPW NLKKTDPEKM SEVLYTLLEV LRYIAIMLQP FIPSSANKML DQLGVNKEER 

       490        500        510 
LFKHLIRDYA LRAGSSILEP AIIFPRFEGD VV 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61086.1.
RefSeqYP_246251.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UMX2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0235.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61086; AAY61086; RF_0235.
GeneID3401276.
KEGGrfe:RF_0235.
PATRIC17890858. VBIRicFel64634_0270.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHOG000200401.
KOK01874.
OMAEHGQKMQ.
OrthoDBEOG6CVV9B.
ProtClustDBPRK11893.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-242-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. SSF47323. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYM_RICFE
AccessionPrimary (citable) accession number: Q4UMX2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries