Q4UMW4 (UBIE_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquinone/menaquinone biosynthesis methyltransferase ubiE
EC=2.1.1.163
EC=2.1.1.201

Alternative name(s):
2-methoxy-6-polyprenyl-1,4-benzoquinol methylase
Demethylmenaquinone methyltransferase

Gene names

Name:	ubiE
Ordered Locus Names:	RF_0243

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methyltransferase required for the conversion of demethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) By similarity. HAMAP-Rule MF_01813
Catalytic activity	A demethylmenaquinone + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine. HAMAP-Rule MF_01813 S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol. HAMAP-Rule MF_01813
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 8/8. HAMAP-Rule MF_01813 Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP-Rule MF_01813
Sequence similarities	Belongs to the methyltransferase superfamily. UbiE family.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis Ubiquinone biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	menaquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway ubiquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Ubiquinone/menaquinone biosynthesis methyltransferase ubiE HAMAP-Rule MF_01813		PRO_0000272374

Sequences

	Sequence		Length	Mass (Da)
	Q4UMW4 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: FAE7B7FEC863471F	FASTA	248	28,296
	10 20 30 40 50 60 MNQTNFGFKK VDYTKKQGLV NSVFSNVADK YDLMNDLMSF GLHRLWKDEF SRQIPNLNSH 70 80 90 100 110 120 ILDVASGSGD IALKLAKKAR DRGNNIALTL SDINEEMLKN AKKKAIDLNL FQNLKFTVAS 130 140 150 160 170 180 AEELPFSDNS FDYYTIAFGI RNVPDINKAL KEAYRVLKPM GKFICLEFSK VKESYFKDFY 190 200 210 220 230 240 KFYSFSIIPT IGQAITGNKE AYEYLVESIE LFPSQDEFRI MLKEAGFEEV SYKNLSGGIV AIHSAYKI

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61094.1.
RefSeq	YP_246259.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UMW4.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0243.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61094; AAY61094; RF_0243.
GeneID	3400692.
KEGG	rfe:RF_0243.
PATRIC	17890874. VBIRicFel64634_0278.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2226.
HOGENOM	HOG000249463.
KO	K03183.
OMA	PYQYLVE.
ProtClustDB	PRK00216.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-250-MONOMER.
UniPathway	UPA00079; UER00169. UPA00232.
Family and domain databases
HAMAP	MF_01813. UbiE_methyltran.
InterPro	IPR004033. UbiE/COQ5_MeTrFase. IPR023576. UbiE/COQ5_MeTrFase_CS. IPR025765. UbiE/COQ5_MeTrFase_dom. [Graphical view]
PANTHER	PTHR10108:SF24. PTHR10108:SF24. 1 hit.
Pfam	PF01209. Ubie_methyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITE	PS01183. UBIE_1. 1 hit. PS01184. UBIE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBIE_RICFE

Accession

Primary (citable) accession number: Q4UMW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program