Q4UMS6 (RL2_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L2

Gene names

Name:	rplB
Ordered Locus Names:	RF_0281

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome By similarity. HAMAP-Rule MF_01320
Subunit structure	Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L2P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	large ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	50S ribosomal protein L2 HAMAP-Rule MF_01320		PRO_0000237235

Sequences

	Sequence		Length	Mass (Da)
	Q4UMS6 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: FDF13E778648B777	FASTA	273	30,242
	10 20 30 40 50 60 MALKNFNPIT PSLRELVQVD KTSLWKGRPL KSLTKGISKT GGRNNQGRIT SWHRGGGHKK 70 80 90 100 110 120 LYRIIDFKRN KIDISAIVER IEYDPNRTAF IALIKYEDGE YSYILAPQKL SVGDRVISSQ 130 140 150 160 170 180 DADIKIGNCL PLKFIPTGTT LHNVEMKVGK GGQIARSAGT SVDLVGKDSG YAQIKLRSGE 190 200 210 220 230 240 FRLVPLDCKA TIGSISNPDQ KNINLGKAGR NRWLGWRPHV RGVAMNPVDH PHGGGEGKTS 250 260 270 GGRHPVTPWG FPTKGKKTRK NKRTSKFIVK KRK

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61132.1.
RefSeq	YP_246297.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UMS6.
SMR	Q4UMS6. Positions 8-272.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0281.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61132; AAY61132; RF_0281.
GeneID	3400244.
KEGG	rfe:RF_0281.
PATRIC	17890974. VBIRicFel64634_0324.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0090.
HOGENOM	HOG000232982.
KO	K02886.
OMA	LVRQECM.
ProtClustDB	PRK09374.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-291-MONOMER.
Family and domain databases
Gene3D	2.30.30.30. 1 hit. 2.40.50.140. 1 hit. 4.10.950.10. 1 hit.
HAMAP	MF_01320_B. Ribosomal_L2_B.
InterPro	IPR012340. NA-bd_OB-fold. IPR022666. Rbsml_prot_L2_RNA-bd_dom. IPR014722. Rib_L2_dom2. IPR002171. Ribosomal_L2. IPR005880. Ribosomal_L2_bac/org-type. IPR022669. Ribosomal_L2_C. IPR022671. Ribosomal_L2_CS. IPR014726. Ribosomal_L2_dom3. IPR008991. Translation_prot_SH3-like. [Graphical view]
PANTHER	PTHR13691. PTHR13691. 1 hit. PTHR13691:SF5. PTHR13691:SF5. 1 hit.
Pfam	PF00181. Ribosomal_L2. 1 hit. PF03947. Ribosomal_L2_C. 1 hit. [Graphical view]
PIRSF	PIRSF002158. Ribosomal_L2. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR01171. rplB_bact. 1 hit.
PROSITE	PS00467. RIBOSOMAL_L2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL2_RICFE

Accession

Primary (citable) accession number: Q4UMS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program