Q4UMQ9 (SECY_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein translocase subunit SecY

Gene names

Name:	secY
Ordered Locus Names:	RF_0298

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently By similarity. HAMAP-Rule MF_01465
Subunit structure	Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_01465.
Sequence similarities	Belongs to the SecY/SEC61-alpha family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	intracellular protein transmembrane transport Inferred from electronic annotation. Source: HAMAP protein targeting Inferred from electronic annotation. Source: HAMAP protein transport by the Sec complex Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Protein translocase subunit SecY HAMAP-Rule MF_01465		PRO_0000277281
Regions
	Transmembrane	17 – 37	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	117 – 137	21	Helical; Potential
	Transmembrane	141 – 161	21	Helical; Potential
	Transmembrane	184 – 204	21	Helical; Potential
	Transmembrane	212 – 232	21	Helical; Potential
	Transmembrane	268 – 288	21	Helical; Potential
	Transmembrane	310 – 330	21	Helical; Potential
	Transmembrane	366 – 386	21	Helical; Potential
	Transmembrane	388 – 408	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4UMQ9 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 628CA6BD2C652452	FASTA	433	47,351
	10 20 30 40 50 60 MGQNFSKKSG NDLVSRIVFT ILILIVCRFG SFIPIPGIDS IALSSVAEKN QSGILGMFNM 70 80 90 100 110 120 LSGGSLGRMS IFALAIMPYI TASIIIQLMS VAYKPLENLK KEGEVGKRKV NQLSRYLTVL 130 140 150 160 170 180 LASFQAYGVA ISLESIVTNT GPVVILAGFF FRVTTVITLV VGTMLLMWLG EQITQRGIGN 190 200 210 220 230 240 GTSLIIFIGI ISGVPSAIIS MFELSRKGAL SPLIALAVCI GVVVLIAIII FFEKAQRKLL 250 260 270 280 290 300 VQYPKRQVGN KIYGGEATHM PLKLNTSGVI PPIFASSILL FPATLANFSN SNSETMGMLT 310 320 330 340 350 360 YYLGHGKPVY ILLYVALIMF FSFFYTAIVF NSEETANNLR KYGAYIPGKR PGKNTSDYFD 370 380 390 400 410 420 YILTRLTVIG GIYLSVICVI PELLMNKYVI SLSLGGTSFL IVVNVVLDTM TQIQTYLFSS 430 KYEGLMKKVK LKN

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61149.1.
RefSeq	YP_246314.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4UMQ9.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0298.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61149; AAY61149; RF_0298.
GeneID	3401631.
KEGG	rfe:RF_0298.
PATRIC	17891008. VBIRicFel64634_0341.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0201.
HOGENOM	HOG000080585.
KO	K03076.
OMA	FIMWLGE.
ProtClustDB	PRK09204.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-308-MONOMER.
Family and domain databases
Gene3D	1.10.3370.10. 1 hit.
HAMAP	MF_01465. SecY.
InterPro	IPR026593. SecY. IPR002208. SecY/SEC61-alpha. IPR023201. SecY_su_dom. [Graphical view]
PANTHER	PTHR10906. PTHR10906. 1 hit.
Pfam	PF00344. SecY. 1 hit. [Graphical view]
PIRSF	PIRSF004557. SecY. 1 hit.
SUPFAM	SSF103491. SecY. 1 hit.
TIGRFAMs	TIGR00967. 3a0501s007. 1 hit.
PROSITE	PS00755. SECY_1. 1 hit. PS00756. SECY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SECY_RICFE

Accession

Primary (citable) accession number: Q4UMQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program