Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q4UMJ0 (MFD_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription-repair-coupling factor

Short name=TRCF
EC=3.6.4.-
Gene names
Name:mfd
Ordered Locus Names:RF_0367
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length1142 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site By similarity. HAMAP-Rule MF_00969

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00969.

Sequence similarities

In the N-terminal section; belongs to the UvrB family.

In the C-terminal section; belongs to the helicase family. RecG subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11421142Transcription-repair-coupling factor HAMAP-Rule MF_00969
PRO_0000281073

Regions

Domain592 – 757166Helicase ATP-binding
Domain778 – 932155Helicase C-terminal
Nucleotide binding605 – 6128ATP Potential
Motif710 – 7134DEEQ box HAMAP-Rule MF_00969

Sequences

Sequence LengthMass (Da)Tools
Q4UMJ0 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: CC689B86E2253EC0

FASTA1,142129,663
        10         20         30         40         50         60 
MLQQKFPATA KSFFATHNFT KNLKQDFILS SSNEDEALQL YKQALFFSSN ENIYYFPSYD 

        70         80         90        100        110        120 
TIPYDHTSPN ANILSRRAEI LTKLTTNNSN GKLLITYAAN LLNKLPPKDF FSKYFLKLSP 

       130        140        150        160        170        180 
KMKFTTDELA MFLVENSFTR NASSIDVGEF AVRGEIIDII LPGPKAYRIN FSWDYIESIK 

       190        200        210        220        230        240 
EFDIDTQIST KSCSELVISP ANEIVLNSET NGNFKNNYLR NFGVNHTDNP LYEAVISGRK 

       250        260        270        280        290        300 
FAGYEQLLPL FYNSCSSLVD YLNDPIFIFD NLSKQAILEF EHSYNDFYSA RSEVNKLKFN 

       310        320        330        340        350        360 
SFYPTLSPTD LYFTASEITE LLEQKNNIFI SYENSEQATL IENISSASFI EKKTVFDKLF 

       370        380        390        400        410        420 
EIIKAHSSKK IVIGAASLSG LERIKSITQN YEYKYNEINK LDEAKASVIN VGIIPLNQSF 

       430        440        450        460        470        480 
YTKEYLFITA SELLEEKSSS TNTNKKLKNI LLELDNLAEG EFVVHKDHGI GQFLKLEALE 

       490        500        510        520        530        540 
IKGKLHDFLK ILYAGNDKLY IPVENIEVIK KYGNDNAELD KLGSSSWQRS KAKLKNRIKE 

       550        560        570        580        590        600 
IALHLIQIAA KRKLNSSASV EFDLEEYDKF CANFPFSETE DQLTAINDIK EDLRNGMLMD 

       610        620        630        640        650        660 
RLICGDVGFG KTEVAMRAVF MVAKSLNEHL PQVAVVVPTT ILCSQHFSRF IERFKGFGLN 

       670        680        690        700        710        720 
IKQLSSVISA KEAKIIRSEL ESGKINIIIG THSLLHKNTK FFNLKLLIID EEQHFGVGQK 

       730        740        750        760        770        780 
EFLKSLKSSS HVLAMSATPI PRTLQMSMTG LKELSIIATP PLNRLEVRTM VMPFDPVIIR 

       790        800        810        820        830        840 
DALLREHFRG GRSFYVVPRI KDIEDIEKQL KQIVPELSYK IAHGKMTPSK IDEVMSEFYA 

       850        860        870        880        890        900 
GKFDILVSTT IIESGIDIAE ANTMVIHKAD MLGLSQLYQL RGRIGRGKVR GYAYLTVASH 

       910        920        930        940        950        960 
KKMTSHSLRR LEIIQNSCAL GSGFTIASHD MDLRGFGNLI GEEQSGQIKE VGTELYQEML 

       970        980        990       1000       1010       1020 
EEQIAIFKDE PIVSEQLFIP TINLGLSVFI PDNYISDSAL KLGLYRRIGN LSNEIEVEKF 

      1030       1040       1050       1060       1070       1080 
KDEMIDRFGS LPIEFNNLLD IVKIKLLCFK LNIENLDSGD NGFVIKFYKN ADMTDKILKF 

      1090       1100       1110       1120       1130       1140 
VSTYSNQAKI KPDNKLVYIK KLVDKNIIVE VNQLLWKLGG CRLDVIPNTS LRGAKRRGNL 


IK 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61218.1.
RefSeqYP_246383.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UMJ0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0367.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61218; AAY61218; RF_0367.
GeneID3400360.
KEGGrfe:RF_0367.
PATRIC17891190. VBIRicFel64634_0429.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1197.
HOGENOMHOG000216592.
KOK03723.
OMANSCALGS.
OrthoDBEOG6FNHKW.
ProtClustDBCLSK871007.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-380-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_00969. TRCF.
InterProIPR003711. CarD-like/TRCF_domain.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR004576. Mfd.
IPR027417. P-loop_NTPase.
IPR005118. TRCF_C.
[Graphical view]
PfamPF02559. CarD_CdnL_TRCF. 1 hit.
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF03461. TRCF. 1 hit.
[Graphical view]
SMARTSM01058. CarD_TRCF. 1 hit.
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00982. TRCF. 1 hit.
[Graphical view]
SUPFAMSSF141259. SSF141259. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsTIGR00580. mfd. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMFD_RICFE
AccessionPrimary (citable) accession number: Q4UMJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2005
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names