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Protein

Transcription-repair-coupling factor

Gene

mfd

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi605 – 612ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription-repair-coupling factorUniRule annotation (EC:3.6.4.-UniRule annotation)
Short name:
TRCFUniRule annotation
Gene namesi
Name:mfdUniRule annotation
Ordered Locus Names:RF_0367
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000008548 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002810731 – 1142Transcription-repair-coupling factorAdd BLAST1142

Proteomic databases

PRIDEiQ4UMJ0

Interactioni

Protein-protein interaction databases

STRINGi315456.RF_0367

Structurei

3D structure databases

ProteinModelPortaliQ4UMJ0
SMRiQ4UMJ0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini592 – 757Helicase ATP-bindingUniRule annotationAdd BLAST166
Domaini778 – 932Helicase C-terminalUniRule annotationAdd BLAST155

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi710 – 713DEEQ box4

Sequence similaritiesi

In the N-terminal section; belongs to the UvrB family.UniRule annotation
In the C-terminal section; belongs to the helicase family. RecG subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JA2 Bacteria
COG1197 LUCA
HOGENOMiHOG000216592
KOiK03723
OMAiLDIPRVN
OrthoDBiPOG091H025E

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di3.90.1150.50, 1 hit
HAMAPiMF_00969 TRCF, 1 hit
InterProiView protein in InterPro
IPR036101 CarD-like/TRCF_dom_sf
IPR003711 CarD-like/TRCF_domain
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR004576 Mfd
IPR027417 P-loop_NTPase
IPR037235 TRCF-like_C
IPR005118 TRCF_C
PfamiView protein in Pfam
PF02559 CarD_CdnL_TRCF, 1 hit
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF03461 TRCF, 1 hit
SMARTiView protein in SMART
SM01058 CarD_TRCF, 1 hit
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00982 TRCF, 1 hit
SUPFAMiSSF141259 SSF141259, 1 hit
SSF143517 SSF143517, 1 hit
SSF52540 SSF52540, 4 hits
TIGRFAMsiTIGR00580 mfd, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q4UMJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQQKFPATA KSFFATHNFT KNLKQDFILS SSNEDEALQL YKQALFFSSN
60 70 80 90 100
ENIYYFPSYD TIPYDHTSPN ANILSRRAEI LTKLTTNNSN GKLLITYAAN
110 120 130 140 150
LLNKLPPKDF FSKYFLKLSP KMKFTTDELA MFLVENSFTR NASSIDVGEF
160 170 180 190 200
AVRGEIIDII LPGPKAYRIN FSWDYIESIK EFDIDTQIST KSCSELVISP
210 220 230 240 250
ANEIVLNSET NGNFKNNYLR NFGVNHTDNP LYEAVISGRK FAGYEQLLPL
260 270 280 290 300
FYNSCSSLVD YLNDPIFIFD NLSKQAILEF EHSYNDFYSA RSEVNKLKFN
310 320 330 340 350
SFYPTLSPTD LYFTASEITE LLEQKNNIFI SYENSEQATL IENISSASFI
360 370 380 390 400
EKKTVFDKLF EIIKAHSSKK IVIGAASLSG LERIKSITQN YEYKYNEINK
410 420 430 440 450
LDEAKASVIN VGIIPLNQSF YTKEYLFITA SELLEEKSSS TNTNKKLKNI
460 470 480 490 500
LLELDNLAEG EFVVHKDHGI GQFLKLEALE IKGKLHDFLK ILYAGNDKLY
510 520 530 540 550
IPVENIEVIK KYGNDNAELD KLGSSSWQRS KAKLKNRIKE IALHLIQIAA
560 570 580 590 600
KRKLNSSASV EFDLEEYDKF CANFPFSETE DQLTAINDIK EDLRNGMLMD
610 620 630 640 650
RLICGDVGFG KTEVAMRAVF MVAKSLNEHL PQVAVVVPTT ILCSQHFSRF
660 670 680 690 700
IERFKGFGLN IKQLSSVISA KEAKIIRSEL ESGKINIIIG THSLLHKNTK
710 720 730 740 750
FFNLKLLIID EEQHFGVGQK EFLKSLKSSS HVLAMSATPI PRTLQMSMTG
760 770 780 790 800
LKELSIIATP PLNRLEVRTM VMPFDPVIIR DALLREHFRG GRSFYVVPRI
810 820 830 840 850
KDIEDIEKQL KQIVPELSYK IAHGKMTPSK IDEVMSEFYA GKFDILVSTT
860 870 880 890 900
IIESGIDIAE ANTMVIHKAD MLGLSQLYQL RGRIGRGKVR GYAYLTVASH
910 920 930 940 950
KKMTSHSLRR LEIIQNSCAL GSGFTIASHD MDLRGFGNLI GEEQSGQIKE
960 970 980 990 1000
VGTELYQEML EEQIAIFKDE PIVSEQLFIP TINLGLSVFI PDNYISDSAL
1010 1020 1030 1040 1050
KLGLYRRIGN LSNEIEVEKF KDEMIDRFGS LPIEFNNLLD IVKIKLLCFK
1060 1070 1080 1090 1100
LNIENLDSGD NGFVIKFYKN ADMTDKILKF VSTYSNQAKI KPDNKLVYIK
1110 1120 1130 1140
KLVDKNIIVE VNQLLWKLGG CRLDVIPNTS LRGAKRRGNL IK
Length:1,142
Mass (Da):129,663
Last modified:July 5, 2005 - v1
Checksum:iCC689B86E2253EC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA Translation: AAY61218.1
RefSeqiWP_011270706.1, NC_007109.1

Genome annotation databases

EnsemblBacteriaiAAY61218; AAY61218; RF_0367
KEGGirfe:RF_0367

Similar proteinsi

Entry informationi

Entry nameiMFD_RICFE
AccessioniPrimary (citable) accession number: Q4UMJ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2005
Last modified: March 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health