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Q4UMI9 (MURE_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
EC=6.3.2.13
Alternative name(s):
Meso-A2pm-adding enzyme
Meso-diaminopimelate-adding enzyme
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
UDP-MurNAc-tripeptide synthetase
UDP-N-acetylmuramyl-tripeptide synthetase
Gene names
Name:murE
Ordered Locus Names:RF_0368
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group ›

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP-Rule MF_00208

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP-Rule MF_00208

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00208

Subcellular location

Cytoplasm By similarity.

Post-translational modification

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP-Rule MF_00208

Sequence similarities

Belongs to the MurCDEF family. MurE subfamily.

Contains 1 RPE1 insert domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 530530UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP-Rule MF_00208
PRO_0000278039

Regions

Domain221 – 26949RPE1 insert
Nucleotide binding99 – 1057ATP Potential
Region145 – 1462UDP-MurNAc-L-Ala-D-Glu binding By similarity
Region446 – 4494Meso-diaminopimelate binding By similarity
Motif446 – 4494Meso-diaminopimelate recognition motif HAMAP-Rule MF_00208

Sites

Binding site211UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1721UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1781UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site1801UDP-MurNAc-L-Ala-D-Glu By similarity
Binding site4221Meso-diaminopimelate By similarity
Binding site4961Meso-diaminopimelate; via carbonyl oxygen By similarity
Binding site5001Meso-diaminopimelate By similarity

Amino acid modifications

Modified residue2121N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UMI9 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: DB0F36484D2E8508

FASTA53059,450
        10         20         30         40         50         60 
MPYNLKQLFQ KHNVKGLSIN SKTVKENDIF FAIKGQNVDG NDFINEVLNQ AVALVITDNK 

        70         80         90        100        110        120 
KNTIIDDKVI YVEDVQVALY EAIEIFYPKK PKNLIAVTGT NGKSSVVSYI AQTYSLLGKK 

       130        140        150        160        170        180 
AASIGTIGVE IFGCDNLIND VPELTTLDYL SFRKIAHNLA ENNIEYLAFE ASSHGLNQAR 

       190        200        210        220        230        240 
LGEIKVNTAC FTSFSQDHLD YHHTKENYLL AKLKLFTRHL FKPAYREEFK GDTEHSTTAY 

       250        260        270        280        290        300 
ILVREDASTG STSKLLLEAK FGKMSTEYLL QGGLAILNSD IAEIEFVKDY LRNHNIKFIT 

       310        320        330        340        350        360 
VGKKGDVQIT KINGSLKAQN INFIFNNREC SFNTSIIGSF QASNLLIAAL SIHYIGFDFN 

       370        380        390        400        410        420 
KIIEILTQVK PVKGRMERIG NTNIFVDYSH TPDALEKALT ELKNVKLRDS RLNVVFGCGG 

       430        440        450        460        470        480 
NRDKTKRSLM GQIAARLADN VIITDDNPRH EDPKLIRAEI ISGIEKADYT EIANREEAIK 

       490        500        510        520        530 
YGINNLKQDD ILLIAGKGHE NYQIIGDKKL PFDDAEIVRK LMSLRGKAKP

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000053 Genomic DNA. Translation: AAY61219.1.
RefSeqYP_246384.1. NC_007109.1.

3D structure databases

HSSPHSSP built from PDB template 1E8C based on UniProtKB P22188.
ProteinModelPortalQ4UMI9.
ModBaseSearch...

Protein-protein interaction databases

STRING315456.RF_0368.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61219; AAY61219; RF_0368.
GeneID3400361.
KEGGrfe:RF_0368.
PATRIC17891192. VBIRicFel64634_0430.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0769.
HOGENOMHOG000268118.
KOK01928.
OMAFEDYLAC.
ProtClustDBPRK00139.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.1190.10. 2 hits.
3.90.190.20. 1 hit.
HAMAPMF_00208. MurE. Divergent sequence.
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005728. Rickett_RPE.
IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase.
[Graphical view]
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 2 hits.
[Graphical view]
SUPFAMSSF53244. Mur_ligase_C. 1 hit.
SSF53623. Mur_ligase_cen. 2 hits.
TIGRFAMsTIGR01085. murE. 1 hit.
TIGR01045. RPE1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURE_RICFE
AccessionPrimary (citable) accession number: Q4UMI9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 5, 2005
Last modified: May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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