Reviewed, UniProtKB/Swiss-Prot Q4UME8 (SYE2_RICFE)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 2
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 2
      Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	RF_0413

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00022
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Glutamyl-tRNA synthetase 2 HAMAP MF_00022		PRO_0000237397
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	239 – 243	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UME8-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 869DFB94C9DC3201	FASTA	463	52,726
	10 20 30 40 50 60 MTNVITRFAP SPTGFLHIGS ARTALFNYLF ARHHNGKFLL RIEDTDKERS TKEAVEAIFS 70 80 90 100 110 120 GLKWLGLDWD GEVIFQSKRN NLYKEAALRL LQEGKAYYCF TSQEEIERQR QKALENKQHF 130 140 150 160 170 180 IFNSEWRDKD SSTYPTDIKP VIRLKVPREG SITIHDTLQG DVVIENSHID DMVLLRADGT 190 200 210 220 230 240 ATYMLAVVVD DHDMGITHII RGDDHLTNAA RQIAIYHAFG YEVPSMTHIP LIHGADGAKL 250 260 270 280 290 300 SKRHGALGVE AYKDMGYLPE SLCNYLLRLG WSHGDDEIIS MTQAIEWFNL DSLGKSPSKL 310 320 330 340 350 360 DFAKMNSLNA HYLRMLDNDS LTSKTVEILK QNYKISEKEV SYIKQAMPSL LVRSETLLDL 370 380 390 400 410 420 AKLARIYLIN SPIIYEQEAK EIIENCDKDL IKQVIEGLNK IEQFDKESVQ NKFKEIATHN 430 440 450 460 GLKLNDIMKP VRALITGMTA SPSVFEIAEI LGKENILKRL KII

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61264.1.
RefSeq	YP_246429.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3400710.
GenomeReviews	Gene locus RF_0413 in contig CP000053_GR.
KEGG	rfe:RF_0413.
NMPDR	fig|315456.3.peg.247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UME8.
OMA	Q4UME8. IEWFNLD.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0413-MON.
BRENDA	6.1.1.17. 297363.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_RICFE

Accession

Primary (citable) accession number: Q4UME8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)