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Q4UMD8

- SYI_RICFE

UniProt

Q4UMD8 - SYI_RICFE

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Protein
Isoleucine--tRNA ligase
Gene
ileS, RF_0423
Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-436-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:RF_0423
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10941094Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098559Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0423.

Structurei

3D structure databases

ProteinModelPortaliQ4UMD8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionUniRule annotation
Add
BLAST
Motifi624 – 6285"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UMD8-1 [UniParc]FASTAAdd to Basket

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MTNTKYYPEV SSNADFAAIE REILKFWQDN NIFQKSIDDR NGESEFIFYD     50
GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ 100
SEKELGISGR LAITNFGIEK FNSHCRASVM KYASDWEEYV TRQARWVDFK 150
NSYKTMDKNF MESVIWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR 200
LDNSYRERAD KAVTVSFVLN EIPAINGMTS RESGTTSSGD YKEYRILAWT 250
TTPWTLPSNL ALAVGSDIDY ASIPKEDICY IIAASSVSKY AKELGLSGEE 300
NFEIIKGSAL QGLSYKPLFD YFANHPNSFK IFAGDFVVEG DGTGIVHMAP 350
GFGEDDQILC ESKGISLVCP VDNSGKFTKE IPDLEGVQVF DANDKIIIKL 400
KEQGNWLKTE QYIHNYPHCW RTDTPLIYKA VPSWYVKVTQ FKDRMVELNQ 450
QINWIPFHVK DNLFGKWLEN ARDWSISRNR FWGTPLPVWK SDDPKYPRID 500
VYGSIEELEK DFGVKVTDLH RPFIDELARP NPNDPTGKST MRRIEDVFDC 550
WFESGSMPYG QAHYPFENKK WFEDHFPADF IVEYSAQTRG WFYTLMVLST 600
ALFDRPPFLN CICHGVILDA TGQKLSKRLN NYADPLELFD KYGSDALRVT 650
MLSSNVVKGQ ELLIDKDGKM VFDTLRLFIK PIWNAYHFFT MYANADSLKG 700
ELNFASQNVL DIYILSKLKI AVNKIEESLD NFDTQTAYHA VSEFFEVLNN 750
WYIRRSRARF WKSEKDADKQ NAYNTLYSCL ETMAIAMSAL VPMISEAIYL 800
GLCHKRPDVI ARKDLSLDEA ISGNSHEIAT ALSVPRNDGS ISVHLCNYPN 850
LSSFEINHEL VATMDNVLDI CSNSLFIRST ENIRVRQPLA TITIISKHND 900
KLKSFEDLIK DEINVKSVIY RDDLENYASK KLSINFPMLG KRLPAKMKEI 950
IAASKKGEWE AIGGGLAICD ETLNSDEYKL VLEPHVHIKG AASFENNSSL 1000
LILDLELTAE LIEEGYARDI VRFIQQARKD ADFSITDRIL IEIISEFDLS 1050
KIIDNYGDFI KEQTLGEFSK NFTPDYVSKV ELENHQIQLK VKRS 1094
Length:1,094
Mass (Da):125,428
Last modified:July 5, 2005 - v1
Checksum:i8C4804C46D115EB6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61274.1.
RefSeqiYP_246439.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61274; AAY61274; RF_0423.
GeneIDi3400708.
KEGGirfe:RF_0423.
PATRICi17891332. VBIRicFel64634_0500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000053 Genomic DNA. Translation: AAY61274.1 .
RefSeqi YP_246439.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UMD8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_0423.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY61274 ; AAY61274 ; RF_0423 .
GeneIDi 3400708.
KEGGi rfe:RF_0423.
PATRICi 17891332. VBIRicFel64634_0500.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-436-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSYI_RICFE
AccessioniPrimary (citable) accession number: Q4UMD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi