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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-436-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RF_0423
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10941094Isoleucine--tRNA ligasePRO_0000098559Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0423.

Structurei

3D structure databases

ProteinModelPortaliQ4UMD8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi624 – 6285"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UMD8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNTKYYPEV SSNADFAAIE REILKFWQDN NIFQKSIDDR NGESEFIFYD
60 70 80 90 100
GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ
110 120 130 140 150
SEKELGISGR LAITNFGIEK FNSHCRASVM KYASDWEEYV TRQARWVDFK
160 170 180 190 200
NSYKTMDKNF MESVIWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR
210 220 230 240 250
LDNSYRERAD KAVTVSFVLN EIPAINGMTS RESGTTSSGD YKEYRILAWT
260 270 280 290 300
TTPWTLPSNL ALAVGSDIDY ASIPKEDICY IIAASSVSKY AKELGLSGEE
310 320 330 340 350
NFEIIKGSAL QGLSYKPLFD YFANHPNSFK IFAGDFVVEG DGTGIVHMAP
360 370 380 390 400
GFGEDDQILC ESKGISLVCP VDNSGKFTKE IPDLEGVQVF DANDKIIIKL
410 420 430 440 450
KEQGNWLKTE QYIHNYPHCW RTDTPLIYKA VPSWYVKVTQ FKDRMVELNQ
460 470 480 490 500
QINWIPFHVK DNLFGKWLEN ARDWSISRNR FWGTPLPVWK SDDPKYPRID
510 520 530 540 550
VYGSIEELEK DFGVKVTDLH RPFIDELARP NPNDPTGKST MRRIEDVFDC
560 570 580 590 600
WFESGSMPYG QAHYPFENKK WFEDHFPADF IVEYSAQTRG WFYTLMVLST
610 620 630 640 650
ALFDRPPFLN CICHGVILDA TGQKLSKRLN NYADPLELFD KYGSDALRVT
660 670 680 690 700
MLSSNVVKGQ ELLIDKDGKM VFDTLRLFIK PIWNAYHFFT MYANADSLKG
710 720 730 740 750
ELNFASQNVL DIYILSKLKI AVNKIEESLD NFDTQTAYHA VSEFFEVLNN
760 770 780 790 800
WYIRRSRARF WKSEKDADKQ NAYNTLYSCL ETMAIAMSAL VPMISEAIYL
810 820 830 840 850
GLCHKRPDVI ARKDLSLDEA ISGNSHEIAT ALSVPRNDGS ISVHLCNYPN
860 870 880 890 900
LSSFEINHEL VATMDNVLDI CSNSLFIRST ENIRVRQPLA TITIISKHND
910 920 930 940 950
KLKSFEDLIK DEINVKSVIY RDDLENYASK KLSINFPMLG KRLPAKMKEI
960 970 980 990 1000
IAASKKGEWE AIGGGLAICD ETLNSDEYKL VLEPHVHIKG AASFENNSSL
1010 1020 1030 1040 1050
LILDLELTAE LIEEGYARDI VRFIQQARKD ADFSITDRIL IEIISEFDLS
1060 1070 1080 1090
KIIDNYGDFI KEQTLGEFSK NFTPDYVSKV ELENHQIQLK VKRS
Length:1,094
Mass (Da):125,428
Last modified:July 5, 2005 - v1
Checksum:i8C4804C46D115EB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61274.1.
RefSeqiWP_011270758.1. NC_007109.1.
YP_246439.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61274; AAY61274; RF_0423.
KEGGirfe:RF_0423.
PATRICi17891332. VBIRicFel64634_0500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61274.1.
RefSeqiWP_011270758.1. NC_007109.1.
YP_246439.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4UMD8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_0423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY61274; AAY61274; RF_0423.
KEGGirfe:RF_0423.
PATRICi17891332. VBIRicFel64634_0500.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-436-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSYI_RICFE
AccessioniPrimary (citable) accession number: Q4UMD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2005
Last modified: May 27, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.