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Q4UMD8

- SYI_RICFE

UniProt

Q4UMD8 - SYI_RICFE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-436-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RF_0423
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10941094Isoleucine--tRNA ligasePRO_0000098559Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0423.

Structurei

3D structure databases

ProteinModelPortaliQ4UMD8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi624 – 6285"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UMD8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNTKYYPEV SSNADFAAIE REILKFWQDN NIFQKSIDDR NGESEFIFYD
60 70 80 90 100
GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ
110 120 130 140 150
SEKELGISGR LAITNFGIEK FNSHCRASVM KYASDWEEYV TRQARWVDFK
160 170 180 190 200
NSYKTMDKNF MESVIWAFKE LYNKGLLYES MRVMPYSWAC ETPLSNFETR
210 220 230 240 250
LDNSYRERAD KAVTVSFVLN EIPAINGMTS RESGTTSSGD YKEYRILAWT
260 270 280 290 300
TTPWTLPSNL ALAVGSDIDY ASIPKEDICY IIAASSVSKY AKELGLSGEE
310 320 330 340 350
NFEIIKGSAL QGLSYKPLFD YFANHPNSFK IFAGDFVVEG DGTGIVHMAP
360 370 380 390 400
GFGEDDQILC ESKGISLVCP VDNSGKFTKE IPDLEGVQVF DANDKIIIKL
410 420 430 440 450
KEQGNWLKTE QYIHNYPHCW RTDTPLIYKA VPSWYVKVTQ FKDRMVELNQ
460 470 480 490 500
QINWIPFHVK DNLFGKWLEN ARDWSISRNR FWGTPLPVWK SDDPKYPRID
510 520 530 540 550
VYGSIEELEK DFGVKVTDLH RPFIDELARP NPNDPTGKST MRRIEDVFDC
560 570 580 590 600
WFESGSMPYG QAHYPFENKK WFEDHFPADF IVEYSAQTRG WFYTLMVLST
610 620 630 640 650
ALFDRPPFLN CICHGVILDA TGQKLSKRLN NYADPLELFD KYGSDALRVT
660 670 680 690 700
MLSSNVVKGQ ELLIDKDGKM VFDTLRLFIK PIWNAYHFFT MYANADSLKG
710 720 730 740 750
ELNFASQNVL DIYILSKLKI AVNKIEESLD NFDTQTAYHA VSEFFEVLNN
760 770 780 790 800
WYIRRSRARF WKSEKDADKQ NAYNTLYSCL ETMAIAMSAL VPMISEAIYL
810 820 830 840 850
GLCHKRPDVI ARKDLSLDEA ISGNSHEIAT ALSVPRNDGS ISVHLCNYPN
860 870 880 890 900
LSSFEINHEL VATMDNVLDI CSNSLFIRST ENIRVRQPLA TITIISKHND
910 920 930 940 950
KLKSFEDLIK DEINVKSVIY RDDLENYASK KLSINFPMLG KRLPAKMKEI
960 970 980 990 1000
IAASKKGEWE AIGGGLAICD ETLNSDEYKL VLEPHVHIKG AASFENNSSL
1010 1020 1030 1040 1050
LILDLELTAE LIEEGYARDI VRFIQQARKD ADFSITDRIL IEIISEFDLS
1060 1070 1080 1090
KIIDNYGDFI KEQTLGEFSK NFTPDYVSKV ELENHQIQLK VKRS
Length:1,094
Mass (Da):125,428
Last modified:July 5, 2005 - v1
Checksum:i8C4804C46D115EB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61274.1.
RefSeqiYP_246439.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61274; AAY61274; RF_0423.
GeneIDi3400708.
KEGGirfe:RF_0423.
PATRICi17891332. VBIRicFel64634_0500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61274.1 .
RefSeqi YP_246439.1. NC_007109.1.

3D structure databases

ProteinModelPortali Q4UMD8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 315456.RF_0423.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAY61274 ; AAY61274 ; RF_0423 .
GeneIDi 3400708.
KEGGi rfe:RF_0423.
PATRICi 17891332. VBIRicFel64634_0500.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RFEL315456:GKEK-436-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSYI_RICFE
AccessioniPrimary (citable) accession number: Q4UMD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2005
Last modified: November 26, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3