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Q4UM78 (THYX_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase ThyX

Short name=TS
Short name=TSase
EC=2.1.1.148
Gene names
Name:thyX
Ordered Locus Names:RF_0491
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate By similarity. HAMAP-Rule MF_01408

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+. HAMAP-Rule MF_01408

Cofactor

Binds 1 FAD per subunit By similarity. HAMAP-Rule MF_01408

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01408

Sequence similarities

Belongs to the thymidylate synthase ThyX family.

Contains 1 thyX (flavin-dependent thymidylate synthase) domain.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   LigandFAD
Flavoprotein
NADP
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

thymidylate synthase (FAD) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294Thymidylate synthase ThyX HAMAP-Rule MF_01408
PRO_0000272379

Regions

Domain27 – 250224ThyX
Motif96 – 10611ThyX motif HAMAP-Rule MF_01408

Sequences

Sequence LengthMass (Da)Tools
Q4UM78 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: BFB8A315D6394E04

FASTA29434,308
        10         20         30         40         50         60 
MHNTTKRVTV PALEEILYEP IKVLDHGFIR VIDYMGDDSA IVQAARVSYG KGTKQLNQDK 

        70         80         90        100        110        120 
GLINYLLRHY HTTPFEMCDI KFHIKLPIFI ARQWIRHRTA SVNEYSARYS ILGNEFYLPE 

       130        140        150        160        170        180 
PANIASQSAV NKQCREGDSL PKEGAEKVLA ILEEDAKQCY GHYKELMNAD EDGNIIDENN 

       190        200        210        220        230        240 
TGIARELARM NLTLNYYTEW YWKINLHNLL HFLRLRADPH AQYEIRVYAE KMLEIVKAWV 

       250        260        270        280        290 
PFTYEAFEEY RLQGANISRK GLDVIKRMIK GEKVTHESSG MTKREWEELM KIFG 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61342.1.
RefSeqYP_246507.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UM78.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0491.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61342; AAY61342; RF_0491.
GeneID3401407.
KEGGrfe:RF_0491.
PATRIC17891484. VBIRicFel64634_0575.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1351.
HOGENOMHOG000021807.
KOK03465.
OMAEIKFHIK.
OrthoDBEOG661H77.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-505-MONOMER.

Family and domain databases

HAMAPMF_01408. ThyX.
InterProIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMSSF69796. SSF69796. 1 hit.
TIGRFAMsTIGR02170. thyX. 1 hit.
PROSITEPS51331. THYX. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTHYX_RICFE
AccessionPrimary (citable) accession number: Q4UM78
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names