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Protein

Thymidylate synthase ThyX

Gene

thyX

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+.UniRule annotation

Cofactori

FADUniRule annotationNote: Binds 1 FAD per subunit.UniRule annotation

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
  2. thymidylate synthase (FAD) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. dTMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-505-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthase ThyXUniRule annotation (EC:2.1.1.148UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyXUniRule annotation
Ordered Locus Names:RF_0491
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294Thymidylate synthase ThyXPRO_0000272379Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0491.

Structurei

3D structure databases

ProteinModelPortaliQ4UM78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 250224ThyXUniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi96 – 10611ThyX motifAdd
BLAST

Sequence similaritiesi

Belongs to the thymidylate synthase ThyX family.UniRule annotation
Contains 1 thyX (flavin-dependent thymidylate synthase) domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1351.
HOGENOMiHOG000021807.
KOiK03465.
OMAiEMCEIKF.
OrthoDBiEOG661H77.

Family and domain databases

HAMAPiMF_01408. ThyX.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UM78-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHNTTKRVTV PALEEILYEP IKVLDHGFIR VIDYMGDDSA IVQAARVSYG
60 70 80 90 100
KGTKQLNQDK GLINYLLRHY HTTPFEMCDI KFHIKLPIFI ARQWIRHRTA
110 120 130 140 150
SVNEYSARYS ILGNEFYLPE PANIASQSAV NKQCREGDSL PKEGAEKVLA
160 170 180 190 200
ILEEDAKQCY GHYKELMNAD EDGNIIDENN TGIARELARM NLTLNYYTEW
210 220 230 240 250
YWKINLHNLL HFLRLRADPH AQYEIRVYAE KMLEIVKAWV PFTYEAFEEY
260 270 280 290
RLQGANISRK GLDVIKRMIK GEKVTHESSG MTKREWEELM KIFG
Length:294
Mass (Da):34,308
Last modified:July 5, 2005 - v1
Checksum:iBFB8A315D6394E04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61342.1.
RefSeqiWP_011270823.1. NC_007109.1.
YP_246507.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61342; AAY61342; RF_0491.
GeneIDi3401407.
KEGGirfe:RF_0491.
PATRICi17891484. VBIRicFel64634_0575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61342.1.
RefSeqiWP_011270823.1. NC_007109.1.
YP_246507.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4UM78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_0491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY61342; AAY61342; RF_0491.
GeneIDi3401407.
KEGGirfe:RF_0491.
PATRICi17891484. VBIRicFel64634_0575.

Phylogenomic databases

eggNOGiCOG1351.
HOGENOMiHOG000021807.
KOiK03465.
OMAiEMCEIKF.
OrthoDBiEOG661H77.

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-505-MONOMER.

Family and domain databases

HAMAPiMF_01408. ThyX.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiTHYX_RICFE
AccessioniPrimary (citable) accession number: Q4UM78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2005
Last modified: February 4, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.