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Protein

Flavin-dependent thymidylate synthase

Gene

thyX

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP+.UniRule annotation

Cofactori

FADUniRule annotationNote: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73FAD; shared with neighboring subunitsUniRule annotation1
Binding sitei104FAD; shared with neighboring subunitsUniRule annotation1
Binding sitei189dUMPUniRule annotation1
Binding sitei211FADUniRule annotation1
Active sitei216Involved in ionization of N3 of dUMP, leading to its activationUniRule annotation1
Binding sitei216dUMP; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi93 – 96dUMP; shared with dimeric partnerUniRule annotation4
Nucleotide bindingi96 – 98FADUniRule annotation3
Nucleotide bindingi104 – 108dUMPUniRule annotation5
Nucleotide bindingi205 – 207FAD; shared with neighboring subunitsUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Flavin-dependent thymidylate synthaseUniRule annotation (EC:2.1.1.148UniRule annotation)
Short name:
FDTSUniRule annotation
Alternative name(s):
FAD-dependent thymidylate synthaseUniRule annotation
Thymidylate synthase ThyXUniRule annotation
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyXUniRule annotation
Ordered Locus Names:RF_0491
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000008548 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002723791 – 294Flavin-dependent thymidylate synthaseAdd BLAST294

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0491.

Structurei

3D structure databases

ProteinModelPortaliQ4UM78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 250ThyXUniRule annotationAdd BLAST224

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 106ThyX motifUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the thymidylate synthase ThyX family.UniRule annotation
Contains 1 thyX (flavin-dependent thymidylate synthase) domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R2U. Bacteria.
COG1351. LUCA.
HOGENOMiHOG000021807.
KOiK03465.
OMAiGMTKREW.
OrthoDBiPOG091H0FIT.

Family and domain databases

HAMAPiMF_01408. ThyX. 1 hit.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4UM78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNTTKRVTV PALEEILYEP IKVLDHGFIR VIDYMGDDSA IVQAARVSYG
60 70 80 90 100
KGTKQLNQDK GLINYLLRHY HTTPFEMCDI KFHIKLPIFI ARQWIRHRTA
110 120 130 140 150
SVNEYSARYS ILGNEFYLPE PANIASQSAV NKQCREGDSL PKEGAEKVLA
160 170 180 190 200
ILEEDAKQCY GHYKELMNAD EDGNIIDENN TGIARELARM NLTLNYYTEW
210 220 230 240 250
YWKINLHNLL HFLRLRADPH AQYEIRVYAE KMLEIVKAWV PFTYEAFEEY
260 270 280 290
RLQGANISRK GLDVIKRMIK GEKVTHESSG MTKREWEELM KIFG
Length:294
Mass (Da):34,308
Last modified:July 5, 2005 - v1
Checksum:iBFB8A315D6394E04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61342.1.
RefSeqiWP_011270823.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61342; AAY61342; RF_0491.
KEGGirfe:RF_0491.
PATRICi17891484. VBIRicFel64634_0575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61342.1.
RefSeqiWP_011270823.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4UM78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_0491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY61342; AAY61342; RF_0491.
KEGGirfe:RF_0491.
PATRICi17891484. VBIRicFel64634_0575.

Phylogenomic databases

eggNOGiENOG4107R2U. Bacteria.
COG1351. LUCA.
HOGENOMiHOG000021807.
KOiK03465.
OMAiGMTKREW.
OrthoDBiPOG091H0FIT.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

HAMAPiMF_01408. ThyX. 1 hit.
InterProiIPR003669. Thymidylate_synthase_ThyX.
[Graphical view]
PfamiPF02511. Thy1. 1 hit.
[Graphical view]
SUPFAMiSSF69796. SSF69796. 1 hit.
TIGRFAMsiTIGR02170. thyX. 1 hit.
PROSITEiPS51331. THYX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHYX_RICFE
AccessioniPrimary (citable) accession number: Q4UM78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.