Reviewed, UniProtKB/Swiss-Prot Q4UM57 (DHAS_RICFE)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:	asd
Ordered Locus Names:	RF_0512

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Aspartate-semialdehyde dehydrogenase		PRO_0000280939
Sites
	Active site	132	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UM57-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 2E74BE813861FBD5	FASTA	338	37,174
	10 20 30 40 50 60 MTKKYNIAVI GATGNVGRET LNILAERNFP VNKIYAIASD NSIGREISYG EKILKISSLT 70 80 90 100 110 120 NLNFDDIDIA FFCAGSEVSK EFVPKATASS CVVIDKSSLF RVDNQVPLIV PEVNLSTLTE 130 140 150 160 170 180 FNTKNIIANP NCIVIPLAVA LKPLDNEIKI KRVVISTYQS VSGAGKAGMD ELYDQTKSKY 190 200 210 220 230 240 VFGENDPKKF PKQITFNLFP HIGDLNKDGY TSEEAKIAFE LNKIMGNHFK ASVTSVRVPV 250 260 270 280 290 300 FIGHSISVNI EFNDKMDANI AEEILQDADG IVTISNNNDL AYISPVEVVG EDAVYVSRIR 310 320 330 NDVSKPNAIN LWITCDNLRK GAALNSVQIA EELINNYL

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61363.1.
RefSeq	YP_246528.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401174.
GenomeReviews	Gene locus RF_0512 in contig CP000053_GR.
KEGG	rfe:RF_0512.
NMPDR	fig|315456.3.peg.346.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UM57.
OMA	Q4UM57. KPLDNEI.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0512-MON.
BRENDA	1.2.1.11. 297363.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR012080. Asp_semialdehyde_DH. IPR005986. Asp_semialdehyde_DH_bac. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR01296. asd_B. 1 hit.
PROSITE	PS01103. ASD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_RICFE

Accession

Primary (citable) accession number: Q4UM57

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)