Q4UM53 (HSLU_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent protease ATPase subunit HslU

Alternative name(s):
Unfoldase HslU

Gene names

Name:	hslU
Ordered Locus Names:	RF_0516

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis By similarity. HAMAP-Rule MF_00249
Subunit structure	A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ClpX chaperone family. HslU subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC protein unfolding Inferred from electronic annotation. Source: HAMAP
Cellular_component	HslUV protease complex Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP ATPase activity Inferred from electronic annotation. Source: HAMAP peptidase activity, acting on L-amino acid peptides Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	ATP-dependent protease ATPase subunit HslU HAMAP-Rule MF_00249		PRO_0000277981
Regions
	Nucleotide binding	71 – 76	6	ATP By similarity
Sites
	Binding site	29	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	261	1	ATP By similarity
	Binding site	328	1	ATP By similarity
	Binding site	400	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UM53 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: B9CC42EEA48D1D9D	FASTA	450	49,557
	10 20 30 40 50 60 MKATKTTYKK DPMGLTPSQI VNELNRFIVG QEKAKKAVAI ALRNRCRRKR VEGNLRNEIV 70 80 90 100 110 120 PKNILMIGST GVGKTEIARR LAKLTNSPFY KIEATKFTEV GYVGRDVESI IRDLVEIAVN 130 140 150 160 170 180 TKKTLAKMEV DINAREKAIE RILDSLVGKT SSSETREKFK EKILNGKLDD TEIEISVADT 190 200 210 220 230 240 TPVGGGSFEI PGMPGASMGV LNLGDMIGRA LGSSKTKTKK MLVKDAMAII IPEESEKLID 250 260 270 280 290 300 QEKIIQQAIN LAENDGIVFI DEIDKIASTG SSGAKNAEIS REGVQRDLLP LIEGTTVNTK 310 320 330 340 350 360 YGPVKTDHIL FIASGAFHIA KPSDLLPELQ GRLPIRVELN SLTKDDMIKI LLEPETSLVK 370 380 390 400 410 420 QYSALIGTED VHLEFAASAI EKIADYAITV NLEVEDIGAR RLHTILENLL EDISFEASEM 430 440 450 KGKKITIDDK FVENQLSKII TNLDLAKFVL

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61367.1.
RefSeq	YP_246532.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 1OFI based on UniProtKB P43773.
ProteinModelPortal	Q4UM53.
SMR	Q4UM53. Positions 14-450.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0516.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61367; AAY61367; RF_0516.
GeneID	3401178.
KEGG	rfe:RF_0516.
PATRIC	17891552. VBIRicFel64634_0609.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1220.
HOGENOM	HOG000010036.
KO	K03667.
OMA	KYGMIKT.
ProtClustDB	PRK05201.
Family and domain databases
HAMAP	MF_00249. HslU.
InterPro	IPR003593. AAA+_ATPase. IPR013093. ATPase_AAA-2. IPR003959. ATPase_AAA_core. IPR019489. Clp_ATPase_C. IPR004491. HslU. [Graphical view]
PANTHER	PTHR11262:SF3. PTHR11262:SF3. 1 hit.
Pfam	PF00004. AAA. 1 hit. PF07724. AAA_2. 1 hit. PF10431. ClpB_D2-small. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM01086. ClpB_D2-small. 1 hit. [Graphical view]
TIGRFAMs	TIGR00390. hslU. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HSLU_RICFE

Accession

Primary (citable) accession number: Q4UM53

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program