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Protein

ATP-dependent protease ATPase subunit HslU

Gene

hslU

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei29 – 291ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei261 – 2611ATPUniRule annotation
Binding sitei328 – 3281ATPUniRule annotation
Binding sitei400 – 4001ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 766ATPUniRule annotation

GO - Molecular functioni

  1. ATPase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. peptidase activity, acting on L-amino acid peptides Source: InterPro

GO - Biological processi

  1. protein unfolding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-530-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent protease ATPase subunit HslUUniRule annotation
Alternative name(s):
Unfoldase HslUUniRule annotation
Gene namesi
Name:hslUUniRule annotation
Ordered Locus Names:RF_0516
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. HslUV protease complex Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450ATP-dependent protease ATPase subunit HslUPRO_0000277981Add
BLAST

Interactioni

Subunit structurei

A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0516.

Structurei

3D structure databases

ProteinModelPortaliQ4UM53.
SMRiQ4UM53. Positions 14-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ClpX chaperone family. HslU subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1220.
HOGENOMiHOG000010036.
KOiK03667.
OMAiRLHTTIE.
OrthoDBiEOG6NPM7G.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00249. HslU.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004491. HslU.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11262:SF3. PTHR11262:SF3. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00390. hslU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4UM53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKATKTTYKK DPMGLTPSQI VNELNRFIVG QEKAKKAVAI ALRNRCRRKR
60 70 80 90 100
VEGNLRNEIV PKNILMIGST GVGKTEIARR LAKLTNSPFY KIEATKFTEV
110 120 130 140 150
GYVGRDVESI IRDLVEIAVN TKKTLAKMEV DINAREKAIE RILDSLVGKT
160 170 180 190 200
SSSETREKFK EKILNGKLDD TEIEISVADT TPVGGGSFEI PGMPGASMGV
210 220 230 240 250
LNLGDMIGRA LGSSKTKTKK MLVKDAMAII IPEESEKLID QEKIIQQAIN
260 270 280 290 300
LAENDGIVFI DEIDKIASTG SSGAKNAEIS REGVQRDLLP LIEGTTVNTK
310 320 330 340 350
YGPVKTDHIL FIASGAFHIA KPSDLLPELQ GRLPIRVELN SLTKDDMIKI
360 370 380 390 400
LLEPETSLVK QYSALIGTED VHLEFAASAI EKIADYAITV NLEVEDIGAR
410 420 430 440 450
RLHTILENLL EDISFEASEM KGKKITIDDK FVENQLSKII TNLDLAKFVL
Length:450
Mass (Da):49,557
Last modified:July 4, 2005 - v1
Checksum:iB9CC42EEA48D1D9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61367.1.
RefSeqiYP_246532.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61367; AAY61367; RF_0516.
KEGGirfe:RF_0516.
PATRICi17891552. VBIRicFel64634_0609.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61367.1.
RefSeqiYP_246532.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4UM53.
SMRiQ4UM53. Positions 14-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_0516.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY61367; AAY61367; RF_0516.
KEGGirfe:RF_0516.
PATRICi17891552. VBIRicFel64634_0609.

Phylogenomic databases

eggNOGiCOG1220.
HOGENOMiHOG000010036.
KOiK03667.
OMAiRLHTTIE.
OrthoDBiEOG6NPM7G.

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-530-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_00249. HslU.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004491. HslU.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11262:SF3. PTHR11262:SF3. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00390. hslU. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiHSLU_RICFE
AccessioniPrimary (citable) accession number: Q4UM53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2007
Last sequence update: July 4, 2005
Last modified: March 31, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.