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Q4UM53 (HSLU_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent protease ATPase subunit HslU
Alternative name(s):
Unfoldase HslU
Gene names
Name:hslU
Ordered Locus Names:RF_0516
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis By similarity. HAMAP-Rule MF_00249

Subunit structure

A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00249.

Sequence similarities

Belongs to the ClpX chaperone family. HslU subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionChaperone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprotein unfolding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentHslUV protease complex

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

peptidase activity, acting on L-amino acid peptides

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450ATP-dependent protease ATPase subunit HslU HAMAP-Rule MF_00249
PRO_0000277981

Regions

Nucleotide binding71 – 766ATP By similarity

Sites

Binding site291ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site2611ATP By similarity
Binding site3281ATP By similarity
Binding site4001ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UM53 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: B9CC42EEA48D1D9D

FASTA45049,557
        10         20         30         40         50         60 
MKATKTTYKK DPMGLTPSQI VNELNRFIVG QEKAKKAVAI ALRNRCRRKR VEGNLRNEIV 

        70         80         90        100        110        120 
PKNILMIGST GVGKTEIARR LAKLTNSPFY KIEATKFTEV GYVGRDVESI IRDLVEIAVN 

       130        140        150        160        170        180 
TKKTLAKMEV DINAREKAIE RILDSLVGKT SSSETREKFK EKILNGKLDD TEIEISVADT 

       190        200        210        220        230        240 
TPVGGGSFEI PGMPGASMGV LNLGDMIGRA LGSSKTKTKK MLVKDAMAII IPEESEKLID 

       250        260        270        280        290        300 
QEKIIQQAIN LAENDGIVFI DEIDKIASTG SSGAKNAEIS REGVQRDLLP LIEGTTVNTK 

       310        320        330        340        350        360 
YGPVKTDHIL FIASGAFHIA KPSDLLPELQ GRLPIRVELN SLTKDDMIKI LLEPETSLVK 

       370        380        390        400        410        420 
QYSALIGTED VHLEFAASAI EKIADYAITV NLEVEDIGAR RLHTILENLL EDISFEASEM 

       430        440        450 
KGKKITIDDK FVENQLSKII TNLDLAKFVL 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61367.1.
RefSeqYP_246532.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UM53.
SMRQ4UM53. Positions 14-450.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0516.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61367; AAY61367; RF_0516.
GeneID3401178.
KEGGrfe:RF_0516.
PATRIC17891552. VBIRicFel64634_0609.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1220.
HOGENOMHOG000010036.
KOK03667.
OMAPVGVEIM.
OrthoDBEOG6NPM7G.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-530-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_00249. HslU.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR019489. Clp_ATPase_C.
IPR004491. HslU.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11262:SF3. PTHR11262:SF3. 1 hit.
PfamPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00390. hslU. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHSLU_RICFE
AccessionPrimary (citable) accession number: Q4UM53
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names