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Q4UM45

- BIOB_RICFE

UniProt

Q4UM45 - BIOB_RICFE

Protein

Biotin synthase

Gene

bioB

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi50 – 501Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi54 – 541Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi94 – 941Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi125 – 1251Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi185 – 1851Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi257 – 2571Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRFEL315456:GKEK-541-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:RF_0527
    OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
    Taxonomic identifieri315456 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000008548: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Biotin synthasePRO_0000381590Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi315456.RF_0527.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4UM45.
    SMRiQ4UM45. Positions 2-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4UM45-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKWTFDEAK EIFSFSFMEL VYQAQTIHRA NFDPNKIQIS SLLSIKTGSC    50
    PENCKFCPQS SHYKTYVKKE PLMQIEDVIT AAKRAKAAGS TRFCMGAAWR 100
    GPRDEDLKLV CEMIKEVKKL GLETCVTLGL LKEHQAVTLK EAGLDFYNHN 150
    IDTSEEFYNK IITTRTFQDR LDTLRYVRAS GMKVCCGGIL GMGETNDDRI 200
    NMILTLANLE EPAESVTINK LIKIPGTPLE NVQDIDPFDF VRVIALARIM 250
    IPKSYIRLSA GREQMSDELQ ALCIMAGVYS IFYGEKILTS ANPMPERDND 300
    LFQKLGIIH 309
    Length:309
    Mass (Da):34,987
    Last modified:July 5, 2005 - v1
    Checksum:i5E7BCC700C4B475B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY61378.1.
    RefSeqiYP_246543.1. NC_007109.1.

    Genome annotation databases

    EnsemblBacteriaiAAY61378; AAY61378; RF_0527.
    GeneIDi3401189.
    KEGGirfe:RF_0527.
    PATRICi17891578. VBIRicFel64634_0622.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000053 Genomic DNA. Translation: AAY61378.1 .
    RefSeqi YP_246543.1. NC_007109.1.

    3D structure databases

    ProteinModelPortali Q4UM45.
    SMRi Q4UM45. Positions 2-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 315456.RF_0527.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAY61378 ; AAY61378 ; RF_0527 .
    GeneIDi 3401189.
    KEGGi rfe:RF_0527.
    PATRICi 17891578. VBIRicFel64634_0622.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci RFEL315456:GKEK-541-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
      Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
      PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC VR-1525 / URRWXCal2.

    Entry informationi

    Entry nameiBIOB_RICFE
    AccessioniPrimary (citable) accession number: Q4UM45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Rickettsia felis (Rickettsia azadi)
      Rickettsia felis (strain URRWXCal2): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3