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Q4UM45 (BIOB_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:RF_0527
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Biotin synthase HAMAP-Rule MF_01694
PRO_0000381590

Sites

Metal binding501Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding541Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding941Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1251Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1851Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2571Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4UM45 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 5E7BCC700C4B475B

FASTA30934,987
        10         20         30         40         50         60 
MKKWTFDEAK EIFSFSFMEL VYQAQTIHRA NFDPNKIQIS SLLSIKTGSC PENCKFCPQS 

        70         80         90        100        110        120 
SHYKTYVKKE PLMQIEDVIT AAKRAKAAGS TRFCMGAAWR GPRDEDLKLV CEMIKEVKKL 

       130        140        150        160        170        180 
GLETCVTLGL LKEHQAVTLK EAGLDFYNHN IDTSEEFYNK IITTRTFQDR LDTLRYVRAS 

       190        200        210        220        230        240 
GMKVCCGGIL GMGETNDDRI NMILTLANLE EPAESVTINK LIKIPGTPLE NVQDIDPFDF 

       250        260        270        280        290        300 
VRVIALARIM IPKSYIRLSA GREQMSDELQ ALCIMAGVYS IFYGEKILTS ANPMPERDND 


LFQKLGIIH 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61378.1.
RefSeqYP_246543.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4UM45.
SMRQ4UM45. Positions 2-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0527.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61378; AAY61378; RF_0527.
GeneID3401189.
KEGGrfe:RF_0527.
PATRIC17891578. VBIRicFel64634_0622.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2309044.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-541-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_RICFE
AccessionPrimary (citable) accession number: Q4UM45
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 5, 2005
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways