Reviewed, UniProtKB/Swiss-Prot Q4UM43 (SYE1_RICFE)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	RF_0529

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00022
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000237396
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UM43-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 55F40BD252E83667	FASTA	447	51,807
	10 20 30 40 50 60 MTKIITRFAP SPTGMLHVGN IRAALLNWLY AKKHNGQFIL RFDDTDLERS KQEYKDAIEE 70 80 90 100 110 120 DLKFLNLNWD QTFNQLSRLS RYDEIKNLLL DKKRLYACYE TPEELELKRK FQLSKGLPPI 130 140 150 160 170 180 YDRASLNLTE EQTQKYIEQG RKPHYRFLVN HEPISWHDMI KGEVKYDGKA LSEPIVIRAD 190 200 210 220 230 240 GSMTYMLCSV IDDIDYDITH IIRGEDHVSN TAIQIQMFEA LNTTPPTFGH LSLIINKDEK 250 260 270 280 290 300 ISKRVGGFEI TTLRKEIGLE AMAIASFFSL LGSSAQILPY KSMEKLANQF EISSFSKSPT 310 320 330 340 350 360 IYQPEDLERL NHKLLISLDF DEVKNHLKEI DAEYIDENFW LSVRPNLQKL RDVKDWWEIC 370 380 390 400 410 420 HKTPNVKSLN LDKEYLKQAA EVLPQGKITK DSWSIWTKEI TNITGKKGKE LFLPLRLALT 430 440 GRESGPEIAS VLPLIDREEI IKRLTSA

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61380.1.
RefSeq	YP_246545.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401191.
GenomeReviews	Gene locus RF_0529 in contig CP000053_GR.
KEGG	rfe:RF_0529.
NMPDR	fig|315456.3.peg.363.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UM43.
OMA	LRLDDTD.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0529-MON.
BRENDA	6.1.1.17. 297363.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_RICFE

Accession

Primary (citable) accession number: Q4UM43

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 5, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)