Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 1

Gene

topA

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Magnesium 1; catalyticUniRule annotation1
Metal bindingi80Magnesium 1; catalyticUniRule annotation1
Metal bindingi80Magnesium 2UniRule annotation1
Metal bindingi82Magnesium 2UniRule annotation1
Active sitei304O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri600 – 627C4-typeAdd BLAST28

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:RF_0530
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000008548 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002731051 – 776DNA topoisomerase 1Add BLAST776

Proteomic databases

PRIDEiQ4UM42

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei31Interaction with DNAUniRule annotation1
Sitei142Interaction with DNAUniRule annotation1
Sitei143Interaction with DNAUniRule annotation1
Sitei146Interaction with DNAUniRule annotation1
Sitei158Interaction with DNAUniRule annotation1
Sitei306Interaction with DNAUniRule annotation1
Sitei499Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi315456.RF_0530

Structurei

3D structure databases

ProteinModelPortaliQ4UM42
SMRiQ4UM42
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 111ToprimUniRule annotationAdd BLAST111

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 171Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri600 – 627C4-typeAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C73 Bacteria
COG0550 LUCA
COG1754 LUCA
HOGENOMiHOG000004020
KOiK03168
OMAiPECKYTR
OrthoDBiPOG091H02BN

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03363 TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00952 Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR013498 Topo_IA_Znf
IPR005733 TopoI_bac-type
IPR028612 Topoisom_1_IA
IPR025589 Toprim_C_rpt
IPR006171 TOPRIM_domain
IPR034149 TOPRIM_TopoI
PANTHERiPTHR42785 PTHR42785, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF13368 Toprim_C_rpt, 2 hits
PF01396 zf-C4_Topoisom, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 2 hits
TIGRFAMsiTIGR01051 topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q4UM42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLVIVESPA KAKTINKYLG DEFKVIASFG HIRDLPSKKG SVLPDENFAM
60 70 80 90 100
KYDISDKAGK YVDAIVKDAK KADAVYLATD PDREGEAISW HVAEVIKEKN
110 120 130 140 150
KVKSDDFFKR VAFNEITKKA IIHAVENPRK LDANLVNAQQ ARRALDYLVG
160 170 180 190 200
FTLSPLLWRK LPGCKSAGRV QSVALRLICE REDEIERFKS EEYWDISLKM
210 220 230 240 250
QNSNNELFTA KLTHVNDQKL EKFSIINEKD AKDLTEKLKS QNFHVDKIEK
260 270 280 290 300
KQQKRQPQPP FITSSLQQEA ARKLGFSAKK TMQIAQKLYE GVDIGKETIG
310 320 330 340 350
LITYMRTDGV TLSNDAIADI RKLIDKNYGD KYLPNSPRIY KSKVKNAQEA
360 370 380 390 400
HEAIRPTNIT YTPDSLKEKL EKDYYKLYEL IWKRTIACQM ENVIMDLVVA
410 420 430 440 450
SLASENKEYL AKANGSTIAF DGFYKVYRES VDDEAEEENK MLPPLKEQEP
460 470 480 490 500
LKTKEIIPNQ HFTEPPPRYS EASLVKKLEE LGIGRPSTYA SILSVLQDRK
510 520 530 540 550
YVSLEKKRFM PEELGRLVTV FLVGFFKKYV EYDFTAGLEN ELDEIAAGKL
560 570 580 590 600
EWKAALNNFW SGFNHNIESV NEQKITEIIS YVQKALDYHL FSENKESKAC
610 620 630 640 650
PSCKTGELSL KLGKFGAFLA CSNYPECTFR KSIVSGNDNN ENDGDLAATP
660 670 680 690 700
NENKVLGTDK DGIEIYLKKG PYGPYVQLGE QEGKVKPKRS PVPASLNQND
710 720 730 740 750
ITLEMALKLL SLPLKIGIHK DSGEEIMIGY GKFGPYIKYM GKFISIPKKY
760 770
DFLNLSLDDA MKLIEDNKAK LEKKQA
Length:776
Mass (Da):88,141
Last modified:July 5, 2005 - v1
Checksum:i7833BDDB544310E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA Translation: AAY61381.1
RefSeqiWP_011270862.1, NC_007109.1

Genome annotation databases

EnsemblBacteriaiAAY61381; AAY61381; RF_0530
KEGGirfe:RF_0530

Similar proteinsi

Entry informationi

Entry nameiTOP1_RICFE
AccessioniPrimary (citable) accession number: Q4UM42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2005
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health