Reviewed, UniProtKB/Swiss-Prot Q4UM42 (TOP1_RICFE)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	RF_0530

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 776	776	DNA topoisomerase 1		PRO_0000273105
Regions
	Zinc finger	600 – 627	28	C4-type
Sites
	Active site	304	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UM42-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 7833BDDB544310E6	FASTA	776	88,141
	10 20 30 40 50 60 MKLVIVESPA KAKTINKYLG DEFKVIASFG HIRDLPSKKG SVLPDENFAM KYDISDKAGK 70 80 90 100 110 120 YVDAIVKDAK KADAVYLATD PDREGEAISW HVAEVIKEKN KVKSDDFFKR VAFNEITKKA 130 140 150 160 170 180 IIHAVENPRK LDANLVNAQQ ARRALDYLVG FTLSPLLWRK LPGCKSAGRV QSVALRLICE 190 200 210 220 230 240 REDEIERFKS EEYWDISLKM QNSNNELFTA KLTHVNDQKL EKFSIINEKD AKDLTEKLKS 250 260 270 280 290 300 QNFHVDKIEK KQQKRQPQPP FITSSLQQEA ARKLGFSAKK TMQIAQKLYE GVDIGKETIG 310 320 330 340 350 360 LITYMRTDGV TLSNDAIADI RKLIDKNYGD KYLPNSPRIY KSKVKNAQEA HEAIRPTNIT 370 380 390 400 410 420 YTPDSLKEKL EKDYYKLYEL IWKRTIACQM ENVIMDLVVA SLASENKEYL AKANGSTIAF 430 440 450 460 470 480 DGFYKVYRES VDDEAEEENK MLPPLKEQEP LKTKEIIPNQ HFTEPPPRYS EASLVKKLEE 490 500 510 520 530 540 LGIGRPSTYA SILSVLQDRK YVSLEKKRFM PEELGRLVTV FLVGFFKKYV EYDFTAGLEN 550 560 570 580 590 600 ELDEIAAGKL EWKAALNNFW SGFNHNIESV NEQKITEIIS YVQKALDYHL FSENKESKAC 610 620 630 640 650 660 PSCKTGELSL KLGKFGAFLA CSNYPECTFR KSIVSGNDNN ENDGDLAATP NENKVLGTDK 670 680 690 700 710 720 DGIEIYLKKG PYGPYVQLGE QEGKVKPKRS PVPASLNQND ITLEMALKLL SLPLKIGIHK 730 740 750 760 770 DSGEEIMIGY GKFGPYIKYM GKFISIPKKY DFLNLSLDDA MKLIEDNKAK LEKKQA

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61381.1.
RefSeq	YP_246546.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401192.
GenomeReviews	Gene locus RF_0530 in contig CP000053_GR.
KEGG	rfe:RF_0530.
NMPDR	fig|315456.3.peg.364.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UM42.
OMA	RKFYGCS.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0530-MON.
BRENDA	5.99.1.2. 297363.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_RICFE

Accession

Primary (citable) accession number: Q4UM42

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)