Q4UM22 (COXX_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoheme IX farnesyltransferase
EC=2.5.1.-

Alternative name(s):
Heme B farnesyltransferase
Heme O synthase

Gene names

Name:	ctaB
Ordered Locus Names:	RF_0550

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group By similarity. HAMAP-Rule MF_00154
Pathway	Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP-Rule MF_00154.
Miscellaneous	Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature By similarity. HAMAP-Rule MF_00154
Sequence similarities	Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.

Ontologies

Keywords
Biological process	Heme biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	heme O biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protoheme IX farnesyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Protoheme IX farnesyltransferase HAMAP-Rule MF_00154		PRO_0000274893
Regions
	Transmembrane	31 – 51	21	Helical; Potential
	Transmembrane	52 – 72	21	Helical; Potential
	Transmembrane	102 – 119	18	Helical; Potential
	Transmembrane	123 – 145	23	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	179 – 199	21	Helical; Potential
	Transmembrane	225 – 245	21	Helical; Potential
	Transmembrane	247 – 267	21	Helical; Potential
	Transmembrane	284 – 304	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4UM22 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: E51B64737D984DB1	FASTA	305	34,261
	10 20 30 40 50 60 MSSLVTPINL DKINHSQSTV KDYILLMKPR VMSLVIFTGF VGIWLAPYSV HPFIAGIAVV 70 80 90 100 110 120 CIALGAGSAG AINMWYDRDI DSLMKRTQKR PIVRGAIEPD EALSFGLITG FFAVFFMALC 130 140 150 160 170 180 VNLLASFLLL FTIFYYICIY TIWLKRRSIQ NIVIGGVSGA LPPVIGYAAV SNTISLESII 190 200 210 220 230 240 LFLIIFIWTP PHSWALALFC NDDYKNCKVP MMPAVKGNLY TKKQILIYSI LLFIVSLMPF 250 260 270 280 290 300 FIGMNNFIYL ITSGILGLVF LYYSGSLFYD TPDNKQAKRF FAYSIFYLFF IFLLLSSTST ISLIS

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61401.1.
RefSeq	YP_246566.1. NC_007109.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0550.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61401; AAY61401; RF_0550.
GeneID	3400329.
KEGG	rfe:RF_0550.
PATRIC	17891640. VBIRicFel64634_0653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0109.
HOGENOM	HOG000237291.
KO	K02301.
OMA	FCAIIGM.
ProtClustDB	PRK04375.
Enzyme and pathway databases
BioCyc	RFEL315456:GKEK-564-MONOMER.
UniPathway	UPA00834; UER00712.
Family and domain databases
HAMAP	MF_00154. CyoE_CtaB.
InterPro	IPR006369. Protohaem_IX_farnesylTrfase. IPR000537. UbiA_prenyltransferase. [Graphical view]
Pfam	PF01040. UbiA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01473. cyoE_ctaB. 1 hit.
PROSITE	PS00943. UBIA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COXX_RICFE

Accession

Primary (citable) accession number: Q4UM22

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	July 5, 2005
Last modified:	May 29, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program