Q4UM09 (NUOE_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit E
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit E
NDH-1 subunit E

Gene names

Name:	nuoE
Ordered Locus Names:	RF_0563

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 2Fe-2S cluster Potential.
Sequence similarities	Belongs to the complex I 24 kDa subunit family.

Ontologies

Keywords
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	NADH-quinone oxidoreductase subunit E		PRO_0000287855
Sites
	Metal binding	88	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	93	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	129	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	133	1	Iron-sulfur (2Fe-2S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4UM09 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 212E48C1B1945973	FASTA	167	19,316
	10 20 30 40 50 60 MSTNFTFDKK NLNLAEDIIK KYPPHGKRSA ILPLLDLAQR QNGGWLPVPA IEYVANMLEM 70 80 90 100 110 120 PYMRAYEVAT FYSMFNLKRV GKYHIQVCTT TPCWLHGSDD IMKICEKKLG IKLKETTEDQ 130 140 150 160 KFTLSEIECL GACVNAPVVQ INDDYYEDLT EEKMEKLIDE YSNDFKN

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61414.1.
RefSeq	YP_246579.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 2AUV based on UniProtKB Q46505.
ProteinModelPortal	Q4UM09.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0563.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61414; AAY61414; RF_0563.
GeneID	3400342.
KEGG	rfe:RF_0563.
PATRIC	17891675. VBIRicFel64634_0670.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1905.
HOGENOM	HOG000257748.
KO	K00334.
OMA	AMNYIAD.
ProtClustDB	PRK07539.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR002023. 2Fe-2S_Ferredox. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR10371. PTHR10371. 1 hit.
Pfam	PF01257. Complex1_24kDa. 1 hit. [Graphical view]
PIRSF	PIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01958. nuoE_fam. 1 hit.
PROSITE	PS01099. COMPLEX1_24K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOE_RICFE

Accession

Primary (citable) accession number: Q4UM09

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program