Reviewed, UniProtKB/Swiss-Prot Q4ULZ7 (FABI_RICFE)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH]
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Ordered Locus Names:	RF_0575

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Enoyl-[acyl-carrier-protein] reductase [NADH]		PRO_0000286636
Regions
	Nucleotide binding	12 – 38	27	NAD By similarity
Sites
	Active site	157	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ULZ7-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 14E5338B63ABDD2C	FASTA	260	28,254
	10 20 30 40 50 60 MTTGLLQGKK GLITGIANNM SISWAIAQLA KKHGAELWFT YQSEVLEKRV KPLAEEIGCN 70 80 90 100 110 120 FVSELDVTNP KSISNLFDDI KEKWGSFDFL LHGMAFADKN ELKGRYVDTS LENFHNSLHI 130 140 150 160 170 180 SCYSLLELSR SAEALMHDGG SIVTLTYYGA EKVIPNYNVM GISKAALEAS VKYLATDLGE 190 200 210 220 230 240 NNIRVNAISA GPIKTLASSA IGDFSTMLKS HAATAPLKRN TTQEDVGGAA VYLFSNLSKG 250 260 VTGEIHYVDC GYNIMGSNKL

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY61426.1.
RefSeq	YP_246591.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401524.
GenomeReviews	Gene locus RF_0575 in contig CP000053_GR.
KEGG	rfe:RF_0575.
NMPDR	fig|315456.3.peg.409.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4ULZ7.
OMA	Q4ULZ7. LVHCLAF.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0575-MON.
BRENDA	1.3.1.9. 297363.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

FABI_RICFE

Accession

Primary (citable) accession number: Q4ULZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)