Q4ULX9 (PYRG_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CTP synthase
EC=6.3.4.2

Alternative name(s):
CTP synthetase
UTP--ammonia ligase

Gene names

Name:	pyrG
Ordered Locus Names:	RF_0593

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	537 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity. HAMAP-Rule MF_01227
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP-Rule MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity. HAMAP-Rule MF_01227
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP-Rule MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' CTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 537	537	CTP synthase HAMAP-Rule MF_01227		PRO_0000266204
Regions
	Domain	290 – 536	247	Glutamine amidotransferase type-1
	Region	1 – 252	252	Aminator domain HAMAP-Rule MF_01227
Sites
	Active site	379	1	Nucleophile By similarity
	Active site	509	1	By similarity
	Active site	511	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4ULX9 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 93E3E8C22478B211	FASTA	537	60,523
	10 20 30 40 50 60 MVRFIFVTGG VVSSLGKGLT AASLAMLLQA KGFKVCVRKL DPYLNVDPGT MNPHEHGEVY 70 80 90 100 110 120 VTDDGAETDL DLGHYERFTG VSSCKFDSIT TGAIYSKLLK DERLGNYTGV TVQIIPHVTN 130 140 150 160 170 180 IIKDFILSNT KGFDFIICEI GGTVGDIEGL PFFEAIRQIG NKLKNENCLF IHLTLLPYVK 190 200 210 220 230 240 TARELKTKPT QHSVKELRAI GITPNILVCR AERNISKSEI DKIALFCNIE SEYVIPAIDQ 250 260 270 280 290 300 KNIYLVPIAY HNSGLDNKVL KFFNINVVPS KLDKWYDIIG RLKDSSSKVR IAIIAKYHKL 310 320 330 340 350 360 KDAYKSVIEA LDHAGIYYKY KIDLVWINAE NLTEENINKK LLNIDGILVP GGFGERATKG 370 380 390 400 410 420 KITAITYART NNIPFFGICF GMQLATIEIA QNLIGIKDAV TEEFKVDGTK IIEKINKNCE 430 440 450 460 470 480 GSKITIENVK KTMRLGSYPC SLVANTIAAN AYKSLEINER HRHRYKFNNE FQNIFEKNGV 490 500 510 520 530 VFSGFSKDEE IVEIIELPEL RWFVGVQFHP EFKSKPFEAH PLFIQFIKAA IEYTLRK

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61444.1.
RefSeq	YP_246609.1. NC_007109.1.
3D structure databases
HSSP	HSSP built from PDB template 2C5M based on UniProtKB P17812.
ProteinModelPortal	Q4ULX9.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0593.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61444; AAY61444; RF_0593.
GeneID	3401542.
KEGG	rfe:RF_0593.
PATRIC	17891753. VBIRicFel64634_0708.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0504.
HOGENOM	HOG000077515.
KO	K01937.
OMA	FEVNPNL.
ProtClustDB	PRK05380.
Enzyme and pathway databases
UniPathway	UPA00159; UER00277.
Family and domain databases
HAMAP	MF_01227. PyrG.
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. [Graphical view]
PANTHER	PTHR11550. PTHR11550. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_RICFE

Accession

Primary (citable) accession number: Q4ULX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program