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Protein

CTP synthase

Gene

pyrG

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.UniRule annotation

Catalytic activityi

ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.UniRule annotation

Enzyme regulationi

Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.UniRule annotation

Pathwayi: CTP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes CTP from UDP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. CTP synthase (pyrG)
This subpathway is part of the pathway CTP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from UDP, the pathway CTP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131Allosteric inhibitor CTP; alternateUniRule annotation
Binding sitei13 – 131UTP; alternateUniRule annotation
Metal bindingi71 – 711MagnesiumUniRule annotation
Binding sitei71 – 711ATPUniRule annotation
Metal bindingi139 – 1391MagnesiumUniRule annotation
Binding sitei222 – 2221Allosteric inhibitor CTP; alternateUniRule annotation
Binding sitei222 – 2221UTP; alternateUniRule annotation
Binding sitei352 – 3521L-glutamine; via carbonyl oxygenUniRule annotation
Active sitei379 – 3791Nucleophile; for glutamine hydrolysisUniRule annotation
Binding sitei403 – 4031L-glutamineUniRule annotation
Binding sitei464 – 4641L-glutamine; via amide nitrogenUniRule annotation
Active sitei509 – 5091UniRule annotation
Active sitei511 – 5111UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 196ATPUniRule annotation
Nucleotide bindingi146 – 1483Allosteric inhibitor CTPUniRule annotation
Nucleotide bindingi186 – 1916Allosteric inhibitor CTP; alternateUniRule annotation
Nucleotide bindingi186 – 1916UTP; alternateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-608-MONOMER.
UniPathwayiUPA00159; UER00277.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP synthaseUniRule annotation (EC:6.3.4.2UniRule annotation)
Alternative name(s):
Cytidine 5'-triphosphate synthaseUniRule annotation
Cytidine triphosphate synthetaseUniRule annotation
Short name:
CTP synthetaseUniRule annotation
Short name:
CTPSUniRule annotation
UTP--ammonia ligaseUniRule annotation
Gene namesi
Name:pyrGUniRule annotation
Ordered Locus Names:RF_0593
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000008548 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537CTP synthasePRO_0000266204Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0593.

Structurei

3D structure databases

ProteinModelPortaliQ4ULX9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini290 – 536247Glutamine amidotransferase type-1UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 265265Amidoligase domainUniRule annotationAdd
BLAST
Regioni380 – 3834L-glutamine bindingUniRule annotation

Sequence similaritiesi

Belongs to the CTP synthase family.UniRule annotation
Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C8D. Bacteria.
COG0504. LUCA.
HOGENOMiHOG000077515.
KOiK01937.
OMAiMRLGEYE.
OrthoDBiEOG6RC3NR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4ULX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRFIFVTGG VVSSLGKGLT AASLAMLLQA KGFKVCVRKL DPYLNVDPGT
60 70 80 90 100
MNPHEHGEVY VTDDGAETDL DLGHYERFTG VSSCKFDSIT TGAIYSKLLK
110 120 130 140 150
DERLGNYTGV TVQIIPHVTN IIKDFILSNT KGFDFIICEI GGTVGDIEGL
160 170 180 190 200
PFFEAIRQIG NKLKNENCLF IHLTLLPYVK TARELKTKPT QHSVKELRAI
210 220 230 240 250
GITPNILVCR AERNISKSEI DKIALFCNIE SEYVIPAIDQ KNIYLVPIAY
260 270 280 290 300
HNSGLDNKVL KFFNINVVPS KLDKWYDIIG RLKDSSSKVR IAIIAKYHKL
310 320 330 340 350
KDAYKSVIEA LDHAGIYYKY KIDLVWINAE NLTEENINKK LLNIDGILVP
360 370 380 390 400
GGFGERATKG KITAITYART NNIPFFGICF GMQLATIEIA QNLIGIKDAV
410 420 430 440 450
TEEFKVDGTK IIEKINKNCE GSKITIENVK KTMRLGSYPC SLVANTIAAN
460 470 480 490 500
AYKSLEINER HRHRYKFNNE FQNIFEKNGV VFSGFSKDEE IVEIIELPEL
510 520 530
RWFVGVQFHP EFKSKPFEAH PLFIQFIKAA IEYTLRK
Length:537
Mass (Da):60,523
Last modified:July 5, 2005 - v1
Checksum:i93E3E8C22478B211
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61444.1.
RefSeqiWP_011270923.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61444; AAY61444; RF_0593.
KEGGirfe:RF_0593.
PATRICi17891753. VBIRicFel64634_0708.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61444.1.
RefSeqiWP_011270923.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4ULX9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_0593.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY61444; AAY61444; RF_0593.
KEGGirfe:RF_0593.
PATRICi17891753. VBIRicFel64634_0708.

Phylogenomic databases

eggNOGiENOG4105C8D. Bacteria.
COG0504. LUCA.
HOGENOMiHOG000077515.
KOiK01937.
OMAiMRLGEYE.
OrthoDBiEOG6RC3NR.

Enzyme and pathway databases

UniPathwayiUPA00159; UER00277.
BioCyciRFEL315456:GKEK-608-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_01227. PyrG.
InterProiIPR029062. Class_I_gatase-like.
IPR004468. CTP_synthase.
IPR017456. CTP_synthase_N.
IPR017926. GATASE.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11550. PTHR11550. 1 hit.
PfamiPF06418. CTP_synth_N. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00337. PyrG. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiPYRG_RICFE
AccessioniPrimary (citable) accession number: Q4ULX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: July 5, 2005
Last modified: July 6, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.UniRule annotation

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.