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Protein

Proline--tRNA ligase

Gene

proS

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-617-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:RF_0600
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Proline--tRNA ligasePRO_0000248916Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0600.

Structurei

3D structure databases

ProteinModelPortaliQ4ULX2.
SMRiQ4ULX2. Positions 1-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000076894.
KOiK01881.
OMAiIQPAELW.
OrthoDBiEOG6TTVMR.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_01570. Pro_tRNA_synth_type2.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023716. Prolyl-tRNA_ligase_IIa_type2.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4ULX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSKYFLPV LKEEPSEAQV TSHKLMLRSG MIRQQAAGIY TWLPLGLKVL
60 70 80 90 100
KNIENIVRSN MNKAGALEVL MPCIQPAHLW MESGRFDNYG KEMLKFQDRH
110 120 130 140 150
DNTLLFGPTN EDMITDIFRH NIKSYKDLPK NLYHIQWKFR DEIRPRFGVM
160 170 180 190 200
RGREFLMKDA YSFDINEENA VKTYNQMYQA YINTFRDLGV FAIPVIADNG
210 220 230 240 250
PIGGNLSHEF HIIAETGEST IYYNKRFKTL KDNPNIDVEE IKSWYAAAEE
260 270 280 290 300
KHDVNKLPIS EQEITSSKGI EVGHIFYIGS KYSVNMNALI NDEHGKLTPV
310 320 330 340 350
EMSSYGIGIS RLVAAIIEAN CNEKGIIWPS NVAPFKVSLI NLNIHDSKCV
360 370 380 390 400
ELAAKVYKEL SAQNIEVLYD DTEARAGSKF ATHDLIGSPY QIIIGPKKAA
410 420
DDIVELKNRK IGVIEDIEVE NLIHYIK
Length:427
Mass (Da):48,693
Last modified:July 5, 2005 - v1
Checksum:i042291D2466AB820
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61451.1.
RefSeqiWP_011270930.1. NC_007109.1.
YP_246616.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61451; AAY61451; RF_0600.
KEGGirfe:RF_0600.
PATRICi17891771. VBIRicFel64634_0715.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61451.1.
RefSeqiWP_011270930.1. NC_007109.1.
YP_246616.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4ULX2.
SMRiQ4ULX2. Positions 1-424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_0600.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY61451; AAY61451; RF_0600.
KEGGirfe:RF_0600.
PATRICi17891771. VBIRicFel64634_0715.

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000076894.
KOiK01881.
OMAiIQPAELW.
OrthoDBiEOG6TTVMR.

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-617-MONOMER.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
HAMAPiMF_01570. Pro_tRNA_synth_type2.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023716. Prolyl-tRNA_ligase_IIa_type2.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiSYP_RICFE
AccessioniPrimary (citable) accession number: Q4ULX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 5, 2005
Last modified: May 27, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.