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Q4ULX2 (SYP_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Proline--tRNA ligase
EC=6.1.1.15
Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS
Gene names
Name:proS
Ordered Locus Names:RF_0600
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group ›

Protein attributes

Sequence length427 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP-Rule MF_01570

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01570

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: HAMAP

proline-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 427427Proline--tRNA ligase HAMAP-Rule MF_01570
PRO_0000248916

Sequences

Sequence LengthMass (Da)Tools
Q4ULX2 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 042291D2466AB820

FASTA42748,693
        10         20         30         40         50         60 
MLLSKYFLPV LKEEPSEAQV TSHKLMLRSG MIRQQAAGIY TWLPLGLKVL KNIENIVRSN 

        70         80         90        100        110        120 
MNKAGALEVL MPCIQPAHLW MESGRFDNYG KEMLKFQDRH DNTLLFGPTN EDMITDIFRH 

       130        140        150        160        170        180 
NIKSYKDLPK NLYHIQWKFR DEIRPRFGVM RGREFLMKDA YSFDINEENA VKTYNQMYQA 

       190        200        210        220        230        240 
YINTFRDLGV FAIPVIADNG PIGGNLSHEF HIIAETGEST IYYNKRFKTL KDNPNIDVEE 

       250        260        270        280        290        300 
IKSWYAAAEE KHDVNKLPIS EQEITSSKGI EVGHIFYIGS KYSVNMNALI NDEHGKLTPV 

       310        320        330        340        350        360 
EMSSYGIGIS RLVAAIIEAN CNEKGIIWPS NVAPFKVSLI NLNIHDSKCV ELAAKVYKEL 

       370        380        390        400        410        420 
SAQNIEVLYD DTEARAGSKF ATHDLIGSPY QIIIGPKKAA DDIVELKNRK IGVIEDIEVE 


NLIHYIK

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References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000053 Genomic DNA. Translation: AAY61451.1.
RefSeqYP_246616.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4ULX2.
SMRQ4ULX2. Positions 1-424.
ModBaseSearch...

Protein-protein interaction databases

STRING315456.RF_0600.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61451; AAY61451; RF_0600.
GeneID3401117.
KEGGrfe:RF_0600.
PATRIC17891771. VBIRicFel64634_0715.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0442.
HOGENOMHOG000076894.
KOK01881.
OMAIQPAELW.
ProtClustDBPRK12325.

Family and domain databases

Gene3D3.40.50.800. 1 hit.
HAMAPMF_01570. Pro_tRNA_synth_type2.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023716. Prolyl-tRNA_ligase_IIa_type2.
[Graphical view]
PANTHERPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. Anticodon_bd. 1 hit.
TIGRFAMsTIGR00409. proS_fam_II. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYP_RICFE
AccessionPrimary (citable) accession number: Q4ULX2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 5, 2005
Last modified: May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

SIMILARITY comments

Index of protein domains and families

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