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Protein

Holliday junction ATP-dependent DNA helicase RuvA

Gene

ruvA

Organism
Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-621-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvAUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvAUniRule annotation
Ordered Locus Names:RF_0604
OrganismiRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi)
Taxonomic identifieri315456 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000008548 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 203203Holliday junction ATP-dependent DNA helicase RuvAPRO_0000224902Add
BLAST

Interactioni

Subunit structurei

Forms a complex with RuvB.UniRule annotation

Protein-protein interaction databases

STRINGi315456.RF_0604.

Structurei

3D structure databases

ProteinModelPortaliQ4ULW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0632.
HOGENOMiHOG000057115.
KOiK03550.
OMAiYTYQYVR.
OrthoDBiEOG679THG.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00031. DNA_helic_RuvA.
InterProiIPR011114. DNA_helicas_Holl-junc_RuvA_C.
IPR013849. DNA_helicase_Holl-junc_RuvA_I.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR000085. RuvA.
IPR010994. RuvA_2-like.
[Graphical view]
PfamiPF07499. RuvA_C. 1 hit.
PF01330. RuvA_N. 1 hit.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46929. SSF46929. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00084. ruvA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4ULW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIGKLSGKID SQGDDYIIID VNGVGYLVYA SGKTLGKLAE GEFYKLFIET
60 70 80 90 100
HVREEHIHLY GFLTLEEKNF FNLLQSVNGI GTRMALSILS SLTPSDIQIA
110 120 130 140 150
INNEDKNIFK AISGVGAKLA ERIVLELKGK VAKISSGSVI IKDSLNIKNI
160 170 180 190 200
TPVASNEVIK ALVNLGFSRF EAQNAVQGII IQNPEISIDE LIKTALKNRN

AGL
Length:203
Mass (Da):22,102
Last modified:July 5, 2005 - v1
Checksum:iF124EEA3E52113DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61455.1.
RefSeqiWP_011270934.1. NC_007109.1.
YP_246620.1. NC_007109.1.

Genome annotation databases

EnsemblBacteriaiAAY61455; AAY61455; RF_0604.
KEGGirfe:RF_0604.
PATRICi17891779. VBIRicFel64634_0719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000053 Genomic DNA. Translation: AAY61455.1.
RefSeqiWP_011270934.1. NC_007109.1.
YP_246620.1. NC_007109.1.

3D structure databases

ProteinModelPortaliQ4ULW8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi315456.RF_0604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY61455; AAY61455; RF_0604.
KEGGirfe:RF_0604.
PATRICi17891779. VBIRicFel64634_0719.

Phylogenomic databases

eggNOGiCOG0632.
HOGENOMiHOG000057115.
KOiK03550.
OMAiYTYQYVR.
OrthoDBiEOG679THG.

Enzyme and pathway databases

BioCyciRFEL315456:GKEK-621-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00031. DNA_helic_RuvA.
InterProiIPR011114. DNA_helicas_Holl-junc_RuvA_C.
IPR013849. DNA_helicase_Holl-junc_RuvA_I.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR000085. RuvA.
IPR010994. RuvA_2-like.
[Graphical view]
PfamiPF07499. RuvA_C. 1 hit.
PF01330. RuvA_N. 1 hit.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46929. SSF46929. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00084. ruvA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
    Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
    PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC VR-1525 / URRWXCal2.

Entry informationi

Entry nameiRUVA_RICFE
AccessioniPrimary (citable) accession number: Q4ULW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 5, 2005
Last modified: May 27, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Rickettsia felis (Rickettsia azadi)
    Rickettsia felis (strain URRWXCal2): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.