Q4ULW8 (RUVA_RICFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvA
EC=3.6.4.12

Gene names

Name:	ruvA
Ordered Locus Names:	RF_0604

Organism

Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

315456 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia felis

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB By similarity. HAMAP-Rule MF_00031
Catalytic activity	ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00031
Subunit structure	Forms a complex with RuvB By similarity.
Sequence similarities	Belongs to the RuvA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP
Cellular_component	Holliday junction helicase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW four-way junction helicase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Holliday junction ATP-dependent DNA helicase RuvA HAMAP-Rule MF_00031		PRO_0000224902

Sequences

	Sequence		Length	Mass (Da)
	Q4ULW8 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: F124EEA3E52113DC	FASTA	203	22,102
	10 20 30 40 50 60 MIGKLSGKID SQGDDYIIID VNGVGYLVYA SGKTLGKLAE GEFYKLFIET HVREEHIHLY 70 80 90 100 110 120 GFLTLEEKNF FNLLQSVNGI GTRMALSILS SLTPSDIQIA INNEDKNIFK AISGVGAKLA 130 140 150 160 170 180 ERIVLELKGK VAKISSGSVI IKDSLNIKNI TPVASNEVIK ALVNLGFSRF EAQNAVQGII 190 200 IQNPEISIDE LIKTALKNRN AGL

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000053 Genomic DNA. Translation: AAY61455.1.
RefSeq	YP_246620.1. NC_007109.1.
3D structure databases
ProteinModelPortal	Q4ULW8.
ModBase	Search...
Protein-protein interaction databases
STRING	315456.RF_0604.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAY61455; AAY61455; RF_0604.
GeneID	3401121.
KEGG	rfe:RF_0604.
PATRIC	17891779. VBIRicFel64634_0719.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0632.
HOGENOM	HOG000057115.
KO	K03550.
OMA	EATIFTH.
ProtClustDB	PRK00116.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00031. DNA_helic_RuvA.
InterPro	IPR011114. DNA_helicas_Holl-junc_RuvA_C. IPR013849. DNA_helicase_Holl-junc_RuvA_I. IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif. IPR012340. NA-bd_OB-fold. IPR000085. RuvA. IPR010994. RuvA_2-like. [Graphical view]
Pfam	PF07499. RuvA_C. 1 hit. PF01330. RuvA_N. 1 hit. [Graphical view]
SMART	SM00278. HhH1. 2 hits. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47781. RuvA_2_like. 1 hit. SSF46929. RuvA_C-like. 1 hit.
TIGRFAMs	TIGR00084. ruvA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUVA_RICFE

Accession

Primary (citable) accession number: Q4ULW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 5, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program