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Q4ULS1 (SYL_RICFE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:RF_0651
OrganismRickettsia felis (strain ATCC VR-1525 / URRWXCal2) (Rickettsia azadi) [Complete proteome] [HAMAP]
Taxonomic identifier315456 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000277916

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif602 – 6065"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6051ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4ULS1 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 3231450DC4964218

FASTA83596,428
        10         20         30         40         50         60 
MNQIEQKWQQ IWYDEKAFEV SNESSKPKYY VLEMLPYPSG KIHVGHVRNY SIGDVIARFM 

        70         80         90        100        110        120 
TMQGFNVLHP MGWDAFGLPA ENAAIKNNSH PKDWTYSNIE NMKKQLKSMG FSYDWSREIN 

       130        140        150        160        170        180 
SCDPQYYKHE QKFFLELYER NLAYQKESLV NWDPVDNTVL ANEQVVDGRG WRSGAIVEKR 

       190        200        210        220        230        240 
YLKQWFLKIT DYAEELLNEI QNLKEWPEAV RSMQEKWIGK SIGANFHFKI KDNEDNTIEV 

       250        260        270        280        290        300 
FSTKPETIFG AGFIGIAFNH PIIEKLISKT PEILSFITKC SHITGSSELE KTEKEGVFTG 

       310        320        330        340        350        360 
LYVIHPFDSN IVLPVIITNF VLMDYGTGAV FGCPAHDERD HELAVKMNLP IKQVIETDID 

       370        380        390        400        410        420 
VQKTAYTEDG ILVNSDFLNG LTSNEAKQKV IDEFEKLGIG KRSVYYRLKD WGISRQRFWG 

       430        440        450        460        470        480 
CPIPMIHCEA CGIVPVPCSD LPVTLPDDVS FDGHGNPLDH HPSWKHVNCP KCDKSAIRET 

       490        500        510        520        530        540 
DTFDTFFESS WYFTRYCNSN ATEMTDKKAC DYWLPVDKYI GGIEHAVMHL LYARFFTKVM 

       550        560        570        580        590        600 
NEQNYVSVRE PFKGLFTQGM VLHATYKDEH NNWLYPEEVV KKGNEFFHKE SNNRVVQGRI 

       610        620        630        640        650        660 
EKMSKSKKNL IDLVTMQEQY GADAIRLFVL SDSPPEKDLE WSASGIEGCS RFINKLEHMF 

       670        680        690        700        710        720 
EAIASLIDDV NSEINKELNR LVHFTIKHVA DDIKHFVLNR AIARMRELSN AISAELNKEV 

       730        740        750        760        770        780 
VDVKTVRYGF NVIVQLLNPF IPHITEEIWQ KLGNKERLYN SAFPAFDESM LELDTYVMAV 

       790        800        810        820        830 
QVNGKLRDTY EFKTSASEDE IKQITVNLPK VQKFLEGKEP KKIILVPRKI VNIIV 

« Hide

References

[1]"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite."
Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D.
PLoS Biol. 3:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000053 Genomic DNA. Translation: AAY61502.1.
RefSeqYP_246667.1. NC_007109.1.

3D structure databases

ProteinModelPortalQ4ULS1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING315456.RF_0651.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY61502; AAY61502; RF_0651.
GeneID3400659.
KEGGrfe:RF_0651.
PATRIC17891902. VBIRicFel64634_0780.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycRFEL315456:GKEK-668-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RICFE
AccessionPrimary (citable) accession number: Q4ULS1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 5, 2005
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Rickettsia felis (Rickettsia azadi)

Rickettsia felis (strain URRWXCal2): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries